Effect of cytoplasmic lipid content on in vitro developmental efficiency of bovine IVP embryos

Jeong, Woohyun; Cho, S.J.; Lee, H.S.; Deb, Giovanni; Lee, Y.S.; Kwon, Tae-Soon; Kong, Il-Keun

doi:10.1016/j.theriogenology.2009.04.015

Cited by 75 publications

(62 citation statements)

References 24 publications

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“…Good quality bovine oocytes have high levels of intracellular lipids which appears optimal for this species, as oocytes with low lipid content have reduced developmental potential (Jeong et al 2009). Bovine oocytes that were denuded before maturation had decreased developmental competence and had significantly less lipid content than cumulus enclosed oocytes (Auclair et al 2013), suggesting that cumulus cells help to regulate the accumulation and/or metabolism of intracellular lipids during oocyte maturation.…”

Section: Discussionmentioning

confidence: 99%

Fatty acid metabolism during maturation affects glucose uptake and is essential to oocyte competence

Paczkowski¹,

Schoolcraft²,

Krisher³

2014

Reproduction

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Fatty acid b-oxidation (FAO) is essential for oocyte maturation in mice. The objective of this study was to determine the effect of etomoxir (a FAO inhibitor; 100 mM), carnitine (1 mM), and palmitic acid (1 or 100 mM) during maturation on metabolism and gene expression of the oocyte and cumulus cells, and subsequent embryo development in the mouse. Carnitine significantly increased embryo development, while there was a decrease in development following maturation with 100 mM palmitic acid or etomoxir (P!0.05) treatment. Glucose consumption per cumulus-oocyte complex (COC) was decreased after treatment with carnitine and increased following etomoxir treatment (P!0.05). Intracellular oocyte lipid content was decreased after carnitine or etomoxir exposure (P!0.05). Abundance of Slc2a1 (Glut1) was increased after etomoxir treatment in the oocyte and cumulus cells (P!0.05), suggesting stimulation of glucose transport and potentially the glycolytic pathway for energy production when FAO is inhibited. Abundance of carnitine palmitoyltransferase 2 (Cpt2) tended to increase in oocytes (PZ0.1) after treatment with 100 mM palmitic acid and in cumulus cells after exposure to 1 mM palmitic acid (PZ0.07). Combined with carnitine, 1 mM palmitic acid increased the abundance of Acsl3 (P!0.05) and Cpt2 tended to increase (PZ0.07) in cumulus cells, suggesting FAO was increased during maturation in response to stimulators and fatty acids. In conclusion, fatty acid and glucose metabolism are related to the mouse COC, as inhibition of FAO increases glucose consumption. Stimulation of FAO decreases glucose consumption and lipid stores, positively affecting subsequent embryo development, while an overabundance of fatty acid or reduced FAO negatively affects oocyte quality.

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Section: Discussionmentioning

confidence: 99%

Fatty acid metabolism during maturation affects glucose uptake and is essential to oocyte competence

Paczkowski¹,

Schoolcraft²,

Krisher³

2014

Reproduction

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“…The esterification of fatty acids and storage into lipid droplets may also protect the oocyte against fatty acid-induced lipotoxicity [17]. In line with this, accumulation of neutral storage lipids in oocytes has been related to improved developmental competence [18,19]. The fatty acid composition of esterified lipids in porcine, cow, and sheep oocytes is dominated by palmitic, oleic, and stearic acid and mirrors the composition of free fatty acids present in blood and follicular fluid [8,14,20].…”

Section: Introductionmentioning

confidence: 91%

Oleic Acid Prevents Detrimental Effects of Saturated Fatty Acids on Bovine Oocyte Developmental Competence1

Aardema

Vos

Lolicato

et al. 2011

Biology of Reproduction

248

221

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Mobilization of fatty acids from adipose tissue during metabolic stress will increase the amount of free fatty acids in blood and follicular fluid and, thus, may affect oocyte quality. In this in vitro study, the three predominant fatty acids in follicular fluid (saturated palmitic and stearic acid and unsaturated oleic acid) were presented to maturing oocytes to test whether fatty acids can affect lipid storage of the oocyte and developmental competence postfertilization. Palmitic and stearic acid had a dose-dependent inhibitory effect on the amount of fat stored in lipid droplets and a concomitant detrimental effect on oocyte developmental competence. Oleic acid, in contrast, had the opposite effect, causing an increase of lipid storage in lipid droplets and an improvement of oocyte developmental competence. Remarkably, the adverse effects of palmitic and stearic acid could be counteracted by oleic acid. These results suggest that the ratio and amount of saturated and unsaturated fatty acid is relevant for lipid storage in the maturing oocyte and that this relates to the developmental competence of maturing oocytes.

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“…Bovine oocytes classified into different 'darkness categories' were found to have differences in cleavage and blastocyst development rates in vitro (Jeong et al 2009). Comparison of bovine oocytes with light homogeneous cytoplasm and those with course dark cytoplasm found that palmitic acid was equally prevalent in both types, but that light oocytes contained a higher percentage of oleic and linoleic (18:2) fatty acids, while the darker oocytes had more saturated stearic acid (Kim et al 2001).…”

Section: Lipid Droplets In Oocytesmentioning

confidence: 99%

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

2014

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Metabolism and ATP levels within the oocyte and adjacent cumulus cells are associated with quality of oocyte and optimal development of a healthy embryo. Lipid metabolism provides a potent source of energy and its importance during oocyte maturation is being increasingly recognised. The triglyceride and fatty acid composition of ovarian follicular fluid has been characterised for many species and is influenced by nutritional status (i.e. dietary fat, fasting, obesity and season) as well as lactation in cows. Lipid in oocytes is a primarily triglyceride of specific fatty acids which differ by species, stored in distinct droplet organelles that re-localise during oocyte maturation. The presence of lipids, particularly saturated vs unsaturated fatty acids, in in vitro maturation systems affects oocyte lipid content as well as developmental competence. Triglycerides are metabolised by lipases that have been localised to cumulus cells as well as oocytes. Fatty acids generated by lipolysis are further metabolised by b-oxidation in mitochondria for the production of ATP. b-oxidation is induced in cumulus-oocyte complexes (COCs) by the LH surge, and pharmacological inhibition of b-oxidation impairs oocyte maturation and embryo development. Promoting b-oxidation with L-carnitine improves embryo development in many species. Thus, fatty acid metabolism in the mammalian COC is regulated by maternal physiological and in vitro environmental conditions; and is important for oocyte developmental competence.

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Effect of cytoplasmic lipid content on in vitro developmental efficiency of bovine IVP embryos

Cited by 75 publications

References 24 publications

Fatty acid metabolism during maturation affects glucose uptake and is essential to oocyte competence

Fatty acid metabolism during maturation affects glucose uptake and is essential to oocyte competence

Oleic Acid Prevents Detrimental Effects of Saturated Fatty Acids on Bovine Oocyte Developmental Competence1

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

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