Effect of D-004, a Lipid Extract from the Cuban Royal Palm Fruit, on Atypical Prostate Hyperplasia Induced by Phenylephrine in Rats

Arruzazabala, M.L.; Más, Rosa; Molina, Vivián; Noa, Miríam; Carbajal, Daisy; Mendoza, Nilda

doi:10.2165/00126839-200607040-00003

Cited by 44 publications

(43 citation statements)

References 34 publications

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“…Overall enlargement of the prostate is a sign of BPH, 30,31) which is characterized by increased proliferation of epithelial and stromal cells in the prostate. Hyperplasia of these cells leads to increased prostate weight and compression of the urethral canal, thereby blocking urine flow.…”

Section: Discussionmentioning

confidence: 99%

<i>Quisqualis indica</i> Improves Benign Prostatic Hyperplasia by Regulating Prostate Cell Proliferation and Apoptosis

Wijerathne

Park

Jeong

et al. 2017

Biological & Pharmaceutical Bulletin

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Quisqualis indica (QI) has been used for treating disorders such as stomach pain, constipation, and digestion problem. This study was aimed to evaluate the therapeutic efficacy of QI extract on treating benign prostatic hyperplasia (BPH) in LNCaP human prostate cancer cell line and a testosterone-induced BPH rat model. LNCaP cells were treated with QI plus testosterone propionate (TP), and androgen receptor (AR) and prostate specific antigen (PSA) expression levels were assessed by Western blotting. To induce BPH, the rats were subjected to a daily subcutaneous injection of TP (3 mg/kg) for 4 weeks. The rats in treatment group were orally gavaged with QI (150 mg/kg) together with the TP injection. In-vitro studies showed that TPinduced increases in AR and PSA expression in LNCaP cells were reduced by QI treatment. In BPH-model rats, the prostate weight, testosterone in serum, dihydrotestosterone (DHT) concentration and 5α-reductase type 2 mRNA expression in prostate tissue were significantly reduced following the treatment with QI. TPinduced prostatic hyperplasia and the expression of proliferating cell nuclear antigen (PCNA) and cyclin D1 were significantly attenuated in QI-treated rats. In addition, QI induced apoptosis by up-regulating caspase-3 and -9 activity and decreasing the B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein (Bax) ratio in prostate tissues of BPH rats. Further investigation showed that TP-induced activation of AKT and glycogen synthase kinase 3β (GSK3β) was reduced by QI administration. Therefore, our findings suggest that QI attenuates the BPH state in rats through anti-proliferative and pro-apoptotic activities and might be useful in the clinical treatment of BPH.Key words benign prostatic hyperplasia; dihydrotestosterone; Quisqualis indica; testosterone Benign prostatic hyperplasia (BPH) is a common urogenital disorder that affects up to 85% of males who are over 50 years-old. 1) BPH is characterized by the increased proliferation of epithelial and stromal cells of the prostate.2) BPH generally causes lower urinary tract symptoms (LUTS), such as incomplete bladder emptying, bladder obstruction, bloody urination, and frequent urination.3)The etiology of BPH is not entirely clear. However, the development and incidence of BPH are associated with dysregulation of androgens, and with increased proliferation and decreased apoptosis of cells in the prostate gland.4-9) Testosterone and dihydrotestosterone (DHT) have key roles in the development and growth of the entire male genital tract, and they stimulate differentiation of the prostate gland. 10,11) The adrenal glands and testes synthesize testosterone, and prostatic 5α-reductase type 2 converts it to DHT. 12) DHT then binds to the androgen receptor (AR), which is transported to the nucleus, where it regulates genes important for prostate growth and differentiation. 4) BPH development and progression are associated with activation of the AKT pathway, a major growth factor-mediated signal transduction pathways. 6)Over-stimulation of the ...

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Section: Discussionmentioning

confidence: 99%

<i>Quisqualis indica</i> Improves Benign Prostatic Hyperplasia by Regulating Prostate Cell Proliferation and Apoptosis

Wijerathne

Park

Jeong

et al. 2017

Biological & Pharmaceutical Bulletin

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“…15,16 In previous studies, animals with BPH have shown an increased relative prostate weight. Finasteride or other agents used to treat BPH decrease the relative prostate weight.…”

Section: Hplc Analysismentioning

confidence: 96%

Effects of Melandrium firmum methanolic extract on testosterone-induced benign prostatic hyperplasia in Wistar rats

Lee¹,

Shin²,

Seo³

et al. 2012

Asian J Androl

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Benign prostatic hyperplasia (BPH) is an age-related disease of unknown aetiology characterized by prostatic enlargement coincident with distinct alterations in tissue histomorphology. Instead of therapeutic agents that can cause severe side effects, plant extracts are frequently used to treat BPH. In this study, we investigated whether the Melandrium firmum methanolic extract (MFME) improves BPH, using the testosterone propionate (TP)-induced BPH rat model. Castration was performed via the scrotal route under sodium pentobarbital anaesthesia. BPH in castrated rats was generated via daily subcutaneous injections of TP (3 mg kg 21 ) dissolved in corn oil, for 4 weeks. MFME was administered daily by oral gavage at a dose of 200 mg kg 21 for 4 weeks, along with the TP injections. The control group received injections of corn oil subcutaneously. At the scheduled termination of the experiment, all rats were killed and their prostates weighed; the relative prostate weight (prostate/body weight ratio) was calculated, and histomorphological changes in the prostate were examined. Additionally, we measured the levels of testosterone and dihydrotestosterone (DHT) in the serum and the prostate. Experimentally induced BPH led to marked decreases in the relative prostate weight and the DHT levels in the serum and the prostate. Histologically, BPH was evident in the ventral lobe of the prostate, and MFME treatment suppressed the severity of the lesions. These results indicate that MFME effectively inhibits the development of BPH induced by testosterone in a rat model. Further studies will be needed to identify the compound(s) responsibility for inducing the protective effect against BPH and determine its mechanism of action.

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“…D-004, a lipid extract of the Roystonea regia (Arecaceae fam) fruits also contains a mixture of fatty acids, oleic, lauric, palmitic and myristic being the most abundant. Experimental studies demonstrated that D-004 decreased testosterone (T)-induced prostate hyperplasia in rodents [17][18][19][20], inhibited rat prostate 5-reductase in vitro [21], antagonized 1-adrenoreceptors-mediated responses in vitro and in vivo [22][23][24] and produced antioxidant effects in the prostate tissue of normal and T-treated rats [25,26].…”

Section: Introductionmentioning

confidence: 99%

Comparative Effects of Roystonea Regia (D-004) and Saw Palmetto Lipid Extracts On Blood Oxidative Variables in Men with Benign Prostate Hyperplasia (BPH)

Guzman¹

2013

iosrphr

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Effect of D-004, a Lipid Extract from the Cuban Royal Palm Fruit, on Atypical Prostate Hyperplasia Induced by Phenylephrine in Rats

Cited by 44 publications

References 34 publications

<i>Quisqualis indica</i> Improves Benign Prostatic Hyperplasia by Regulating Prostate Cell Proliferation and Apoptosis

<i>Quisqualis indica</i> Improves Benign Prostatic Hyperplasia by Regulating Prostate Cell Proliferation and Apoptosis

Effects of Melandrium firmum methanolic extract on testosterone-induced benign prostatic hyperplasia in Wistar rats

Comparative Effects of Roystonea Regia (D-004) and Saw Palmetto Lipid Extracts On Blood Oxidative Variables in Men with Benign Prostate Hyperplasia (BPH)

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