Effect of Daily Voluntary Running on<i>In Vivo</i>Insulin Action in Rat Skeletal Muscle and Adipose Tissue as Determined by the Microdialysis Technique

Wp, Yang; Oshida, Yoshiharu; Wu, Wei; Sato, Juichi; Ohsawa, Isao; Sato, Yuya

doi:10.1055/s-2007-972973

Cited by 13 publications

(8 citation statements)

References 11 publications

(24 reference statements)

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“…Metabolic clearance rate for glucose (MCR, ml´kg ±1´m in ±1 ) was then obtained from GDR divided by the corresponding blood glucose concentration. The GDR and the MCR from 60 to 90 min for the euglycaemic clamp procedure were regarded as indices of insulin action in peripheral tissues, since a plateau glucose infusion rate was achieved during this time, as reported previously [14,15]. In a final group of rats N-monomethyl-l-arginine (l-NMMA, Calbiochem-Novabiochem, Nottingham, England) was infused (1 mg × kg ±1 × min ±1 ) in diabetic rats together with C peptide I (0.05 nmol × kg ±1 × min ±1 ) during low-dose insulin infusion according to the same protocol as described above.…”

Section: Animals and Methodsmentioning

confidence: 99%

Rat C peptide I and II stimulate glucose utilization in STZ-induced diabetic rats

Oshida

Kusunoki

et al. 1999

Diabetologia

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The C peptide of proinsulin has since its discovery been considered to be without biological effect of its own [1]. In recent years it has been reported, however, that C peptide increases muscle blood flow, glucose and oxygen uptake of the exercising forearm, decreases urinary albumin excretion and improves autonomic nerve function in patients with Type I (insulin-dependent) diabetes mellitus [2±6]. Moreover, C peptide has been shown to stimulate Na + ,K + -ATPase activity in renal tubular cells [7] and to activate the nitric oxide (NO) synthase of endothelial cells [8]. The rat has two proinsulins and thus two C peptides that differ with regard to two amino acids in the middle segment of the molecule [9]. Rat C peptide I and II are equipotent in their ability to stimulate Na + ,K + -ATPase [7]. Studies on isolated muscle strips from Type I diabetic patients and healthy subjects have confirmed the effect of C peptide on glucose transport in vivo and indicated that the stimulation occurs through pathways that do not include the insulin receptor [10]. C peptide does not, however, stimulate muscle glycogen synthesis in isolated mouse muscle [11]. Recent data show that glucose transport in rat muscle and adipose tissue can be Diabetologia (1999) AbstractAims. To study the effects of physiological concentrations of rat proinsulin C peptide I and II, respectively, on whole body glucose utilization in streptozotocin diabetic and healthy rats. Methods. A sequential insulin clamp procedure was used (insulin infusion rates 3.0 and 30.0 mU × kg ±1 × min ±1 ) in awake animals. C-peptide infusion rates were 0.05 and 0.5 nmol × kg ±1 × min ±1 . Blood glucose was clamped at 7.7 ± 0.3 mmol/l in the diabetic rats and at 3.9 ± 0.1 mmol/l in the healthy rats. Results. In diabetic rats infused at lower rates of C peptide and insulin, glucose utilization increased by 79±90 % (p < 0.001) compared with diabetic animals infused with saline and insulin. Increasing the rate of C-peptide infusion tenfold did not elicit a statistically significant further increase in glucose utilization. C peptide I and II exerted similar effects. The metabolic clearance rate for glucose in the diabetic animals infused with C peptide was not different from that of the healthy rats. During high-dose insulin infusion (30.0 mU´kg ±1´m in ±1 ) glucose utilization increased considerably and no statistically significant C-peptide effects were observed. About 85 % of the increase in glucose utilization induced by C peptide could be blocked by treatment with N-monomethyl-l-arginine. Conclusions/interpretation. Physiological concentrations of homologous C peptide stimulate whole body glucose utilization in diabetic but not in healthy rats. C peptide I and II elicit similar effects. The influence of C peptide on glucose utilization may be mediated by nitric oxide. [Diabetologia (1999) 42: 958±964]

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Section: Animals and Methodsmentioning

confidence: 99%

Rat C peptide I and II stimulate glucose utilization in STZ-induced diabetic rats

Oshida

Kusunoki

et al. 1999

Diabetologia

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“…The high-fat-fed rats were further assigned to either a sedentary (n = 7) or a wheel running (n = 7) group [11,12]. The number of exercise wheel revolutions were recorded every day to determine running distance.…”

Section: Methodsmentioning

confidence: 99%

Effect of Voluntary Wheel-Running on Insulin Sensitivity and Responsiveness in High-Fat-Fed Rats.

Han

Oshida

et al. 2001

Endocr J

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Abstract.The effect of voluntary wheel-running on insulin resistance was studied in high-fat-fed rats. A sequential hyperinsulinemic euglycemic clamp procedure was employed (insulin infusion rates: 3 and 30 mU/kg BW/min) in 14 high-fat-fed rats and 7 chow-fed rats under the awake condition. The high-fat-fed rats were further divided into a sedentary (n=7) and a voluntary wheel-running (n=7) groups. Blood glucose was clamped at the fasting level in each rat. Plasma insulin levels during the 3-and 30-mU/kg BW/min insulin infusions were 40-50 and 450-550 pU/ml, respectively. At both 3 and 30 mU/kg BW/min insulin infusions, high-fat-feeding showed a significant decrease in glucose infusion rate (GIR), compared with the chow-fed rats. However, decreased GIRs were restored by the 4-wk wheel-running and reached similar levels as the chow-fed rats. Therefore, it could be concluded that voluntary wheel-running prevents insulin resistance induced by high-fat feeding.

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“…A rectal thermometer was introduced and the rectal temperature was kept at 37.0 °C by adjusting the distance from a heating lamp. As previously reported [10,11], microdialysis probes, which consisted of a tubular membrane (polycarbonate, outer diameter 0.5 mm, length 10 mm, molecular weight cut-off 5,000-20,000 daltons, Carnegie Medicin, Sweden), were inserted bilaterally into the quadriceps muscles, vastus lateralis, and were also put into the right jugular vein by using the guide for the blood microdialysis.…”

Section: Experimental Protocolmentioning

confidence: 98%

“…We recently reported that the microdialysis technique can be used to measure intramuscular 3-hydroxybutyrate (3-OHBA) [10] and lactate levels [11]. With this technique, therefore, the present study was undertaken to clarify the effect of tetanic contraction (60 tetani/min) on intramuscular 3-OHBA and lactate levels in diabetic and non-diabetic control rats.…”

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confidence: 97%

Intramuscular 3-Hydroxybutyrate Levels after 60 Tetani/Min Contraction in Diabetic and Non-Diabetic Rats.

et al. 1998

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Abstract. Post-exercise hyperketonemia in poorly-controlled diabetic patients is a well recognized phenomenon, but because studies concerning changes in ketone body metabolism in muscle during and/or after exercise are scarce, we measured the intramuscular 3-hydroxybutyrate (3-OHBA) and lactate concentrations in 12 diabetic (streptozotocin-induced; 60 mg/kg, ip) and 13 non-diabetic control rats before and after a 1-h muscle contraction. One thigh tetanic contraction was elicited at a tetanic frequency of 60 tetani/min and the other thigh was kept resting. We used a microdialysis technique in both quadriceps muscles, vastus lateralis, and the right jugular vein. Blood flow in both femoral muscles was also monitored before and after contraction. Dialysate 3-OHBA and lactate levels in contracting and non-contracting muscles and in blood showed a significant increase after contraction (P<0.05) in diabetic rats. In control rats there was no significant change in dialysate 3-OHBA or the lactate concentration in contracting and non-contracting muscles or in blood before and after contraction. A significant increase in contracting muscle blood flow was observed only for the first 5 min after contraction in diabetic and control rats. These results suggest that 3-OHBA uptake in contracting muscle is reduced, and that this phenomenon may play a role in post-exercise ketosis in diabetes.

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Effect of Daily Voluntary Running onIn VivoInsulin Action in Rat Skeletal Muscle and Adipose Tissue as Determined by the Microdialysis Technique

Cited by 13 publications

References 11 publications

Rat C peptide I and II stimulate glucose utilization in STZ-induced diabetic rats

Rat C peptide I and II stimulate glucose utilization in STZ-induced diabetic rats

Effect of Voluntary Wheel-Running on Insulin Sensitivity and Responsiveness in High-Fat-Fed Rats.

Intramuscular 3-Hydroxybutyrate Levels after 60 Tetani/Min Contraction in Diabetic and Non-Diabetic Rats.

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