Effect of Defatted Dabai Pulp Extract in Urine Metabolomics of Hypercholesterolemic Rats

Abstract

A source of functional food can be utilized from a source that might otherwise be considered waste. This study investigates the hypocholesterolemic effect of defatted dabai pulp (DDP) from supercritical carbon dioxide extraction and the metabolic alterations associated with the therapeutic effects of DDP using 1H NMR urinary metabolomic analysis. Male-specific pathogen-free Sprague–Dawley rats were fed with a high cholesterol diet for 30 days to induce hypercholesterolemia. Later, the rats were administered with a 2% DDP treatment diet for another 30 days. Supplementation with the 2% DDP treatment diet significantly reduced the level of total cholesterol (TC), triglyceride, low-density lipoprotein (LDL), and inflammatory markers (C-reactive protein (CRP), interleukin 6 (IL6) and tumour necrosis factor-α (α-TNF)) and significantly increased the level of antioxidant profile (total antioxidant status (TAS), superoxide dismutase (SOD), glutathione peroxide (GPX), and catalase (CAT)) compared with the positive control group (PG) group (p < 0.05). The presence of high dietary fibre (28.73 ± 1.82 g/100 g) and phenolic compounds (syringic acid, 4-hydroxybenzoic acid and gallic acid) are potential factors contributing to the beneficial effect. Assessment of 1H NMR urinary metabolomics revealed that supplementation of 2% of DDP can partially recover the dysfunction in the metabolism induced by hypercholesterolemia via choline metabolism. 1H-NMR-based metabolomic analysis of urine from hypercholesterolemic rats in this study uncovered the therapeutic effect of DDP to combat hypercholesterolemia.

Effects of enteral nutrition with different energy supplies on metabolic changes and organ damage in burned rats

“…The data obtained were imported into SIMCA-P 14.1 (Umetrics, Umea, Sweden) software for principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) [ 27 ]. The OPLS-DA model was subjected to random permutations 200 times, and its R 2 and Q 2 were calculated for evaluation.…”

Quality of Dabai Pulp Oil Extracted by Supercritical Carbon Dioxide and Supplementation in Hypercholesterolemic Rat—A New Alternative Fat

“…We collected fresh dabai fruits (226 kg) from Sarikei Sarawak, Malaysia. The identification and preparation of dabai pulp were performed based on the method described in our previous study [ 11 ]. Freeze-dried dabai pulp (48.92 kg) was subjected to SC-CO 2 extraction.…”

Anxiolytic, antidepressant and antioxidant activity of the methanol extract of Canarium resiniferum leaves

“…2 Extracts or phytoconstituents of Canarium species have demonstrated a wide range of biological effects including hepatoprotective, analgesic, antimicrobial, antihypercholesterolemic, antioxidant, vasorelaxant, antiviral, anti-obesity, antidiabetic, antipyretic, anti-inflammatory, anticancer, α-amylase and α-glucosidase inhibitory activity. 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 To the best of our knowledge, C. resiniferum has yet to be explored for its phytoconstituents and pharmacological activity.…”