Effect of dehydroepiandrosterone on atopic dermatitis-like skin lesions induced by 1-chloro-2,4-dinitrobenzene in mouse

Chan, Chiu‐Po; Liou, Chian-Jiun; Xu, Pei-Yin; Shen, Jiann-Jong; Kuo, Ming‐Ling; Len, Wen-Bin; Chang, Liang-En; Huang, Wen‐Chung

doi:10.1016/j.jdermsci.2013.06.015

Cited by 39 publications

(25 citation statements)

References 46 publications

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“…The results of histological analysis provided further evidence that NIC-CS-NPs with lowdose FK506, compared to Protopic, ameliorated AD-like effects of FK506-NIc-cs-NPs on downregulating serum total Ige levels AD is an IgE-mediated hypersensitive skin disease; the increase of serum total IgE levels is a typical characteristic in DNCB-induced BALB/c mice with AD-like skin lesions. 64,65 As shown in Figure 7, the serum total IgE levels of DNCB group in the present study almost increased 5-fold of that in the control group. After treatment with Protopic or an NIC-CS-NPs vehicle, the serum total IgE levels was significantly downregulated to 3.5-or 2.8-fold that of the control group.…”

Section: Histopathological Analysissupporting

confidence: 63%

Tacrolimus nanoparticles based on chitosan combined with nicotinamide: enhancing percutaneous delivery and treatment efficacy for atopic dermatitis and reducing dose

Yu¹,

Wang²,

Wan³

et al. 2017

IJN

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Background Topical application of tacrolimus (FK506) was effective in the treatment of atopic dermatitis (AD); however, adverse effects frequently occurred with the increase of FK506 dose during long–term treatment. Objective The objective of this project was to develop a hybrid skin targeting system encapsulating FK506 based on nicotinamide (NIC) and chitosan nanoparticles (CS–NPs), ie, FK506–NIC–CS–NPs, which took advantages of both of NIC and CS–NPs to obtain the synergetic effects of percutaneous delivery and treatment efficacy enhancement along with dose reduction. Methods The formulation of FK506–NIC–CS–NPs was optimized and characterized. In vitro and in vivo skin permeation studies were performed. AD–like skin lesions were constructed with BALB/c mice by 1–chloro–2, 4–dinitrobenzene (DNCB)–induced, and FK506–NIC–CS–NPs containing different dose of FK506 were topically administered to treat AD–like skin lesions in comparison with Protopic. Results NIC was found to significantly increase the FK506 EE to 92.2% by CS–NPs. In comparison with commercial FK506 ointment (Protopic), in vitro and in vivo skin permeation studies demonstrated that NIC–CS–NPs system significantly enhanced FK506 permeation through and into the skin, and deposited more FK506 into the skin. The treatment efficacy on clinical symptoms, histological analysis, and molecular biology of the AD–mice demonstrated that NIC–CS–NPs with ~1/3 dose of FK506 of Protopic was superior to that of Protopic, and NIC–CS–NPs vehicle exhibited the adjuvant therapy and moderate anti–AD effects. Conclusion The system of NIC–CS–NPs enhances the permeability of FK506, plays an adjuvant role in anti-AD, reduces the dose of FK506 in treating AD, and is therefore a promising nanoscale system of FK506 for the effective treatment of AD.

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Section: Histopathological Analysissupporting

confidence: 63%

Tacrolimus nanoparticles based on chitosan combined with nicotinamide: enhancing percutaneous delivery and treatment efficacy for atopic dermatitis and reducing dose

Yu¹,

Wang²,

Wan³

et al. 2017

IJN

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“…The dorsal skin of the mice was shaved and sensitized by application to the skin of 0.5% 1-chloro-2,4-dinitrobenzene (DNCB, Sigma-Aldrich, St. Louis, MO, USA) as previously described [ 17 ]. Briefly, BALB/c mice were shaved of dorsal hair and 200 μ L 0.5% DNCB in acetone/olive oil (3 : 1) was applied to the shaved area on experimental days 1–3 ( Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

Danggui Buxue Tang Inhibits 2,4-Dinitrochlorobenzene: Induced Atopic Dermatitis in Mice

Fang

Cheng

Hwang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Danggui Buxue Tang (DBT) is a herbal decoction that has been used in Chinese medicine to enhance qi and blood circulation. Previously, we found that DBT can suppress allergy-related asthma in mice, leading us to hypothesize that DBT might ameliorate allergy disease. In this study, we evaluated whether DBT can attenuate atopic dermatitis (AD) symptoms and have an anti-inflammatory effect on AD-like mice. The dorsal skin of female mice was shaved and sensitized cutaneously (skin smear) with 1-chloro-2,4-dinitrobenzene. Mice were then given various doses of DBT from days 14 to 29 cutaneously. DBT treatment suppressed ear swelling and skin inflammation and decreased mast cell and eosinophil infiltration into skin and ear tissue. DBT also inhibited levels of IgE and Th2-associated cytokine levels in serum. These results demonstrate that cutaneous administration of DBT reduced the development of AD-like skin lesions in mice.

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“…Twenty-four hours later, the mice were sensitized with 200 μL of 1% DNCB in an acetone : olive oil (3:1) solution applied topically to the shaved dorsal skin, twice over one week. Mice were then challenged with 200 μL of 0.4% DNCB similarly applied, twice a week for 5 weeks (Chan et al, 2013;. Two weeks after the first induction of skin inflammation, bee venom or melittin (100, 200 and 500 μg mixed with a placebo) was applied to the dorsal skin five times per week for 4 weeks.…”

Section: Mouse Model Of Admentioning

confidence: 99%

“…Th2 cells secrete the cytokines IL-4 and IL-5, that up-regulate antibody formation via B cells, eosinophils, mast cells and other pathways (Kidd, 2003). IL-4 and IL-13 have established roles in B cell differentiation and class switching, thus providing a link to characteristic elevations of serum IgE levels in AD, as well as eosinophil and mast cell infiltration into the skin (Chan et al, 2013). The pathogenesis of chronic AD is related to a predominance of Th1 cells and IFN-γ, and chronic AD skin lesions undergo tissue remodelling caused by chronic inflammation (Coondoo, 2012).…”

Section: Introductionmentioning

confidence: 99%

Therapeutic effects of bee venom and its major component, melittin, on atopic dermatitis in vivo and in vitro

Kim

et al. 2018

British J Pharmacology

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Background and PurposeAtopic dermatitis (AD) is a multifactorial skin condition with complex interactions of innate and adaptive immune responses. There are several existing therapies for AD, including topical glucocorticosteroids, emollients, phototherapies, calcineurin inhibitors and immunosuppressants, such as cyclosporine A. Although these therapies reduce inflammation, they also cause serious side effects. Therefore, it is necessary to develop new therapeutic approaches for AD treatment without side effects. There are several studies on natural materials or toxins, such as herbs, ginseng extract and snake venom, for AD treatment. However, treatment of AD with bee venom and its major component, melittin has rarely been studied.Experimental ApproachEffects of bee venom and melittin were studied in a model of AD in vivo induced by 1‐chloro‐2,4‐dinitrobenzene (DNCB) in female Balb/c mice and in cultures of human keratinocytes, stimulated by TNF‐α/IFN‐γ. The potential pharmacological effects of bee venom and melittin on these in vivo and in vitro AD‐like skin disease models were studied.Key ResultsBee venom and melittin exhibited potent anti‐atopic activities, shown by decreased AD‐like skin lesions, induced by DNCB in mice. In vitro studies using TNF‐α/IFN‐γ‐stimulated human keratinocytes showed that bee venom and melittin inhibited the increased expression of chemokines, such as CCL17 and CCL22, and pro‐inflammatory cytokines, including IL‐1β, IL‐6 and IFN‐γ, through the blockade of the NF‐κB and STAT signalling pathways.Conclusions and ImplicationsOur results suggest that bee venom and melittin would be suitable for epicutaneous application, as topical administration is often appropriate for the treatment of AD.

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Effect of dehydroepiandrosterone on atopic dermatitis-like skin lesions induced by 1-chloro-2,4-dinitrobenzene in mouse

Cited by 39 publications

References 46 publications

Tacrolimus nanoparticles based on chitosan combined with nicotinamide: enhancing percutaneous delivery and treatment efficacy for atopic dermatitis and reducing dose

Tacrolimus nanoparticles based on chitosan combined with nicotinamide: enhancing percutaneous delivery and treatment efficacy for atopic dermatitis and reducing dose

Danggui Buxue Tang Inhibits 2,4-Dinitrochlorobenzene: Induced Atopic Dermatitis in Mice

Therapeutic effects of bee venom and its major component, melittin, on atopic dermatitis in vivo and in vitro

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