Effect of dengue virus infection on the permeability of vero cells line

Kaliwantoro, Nur; Soesatyo, Marsetyawan Hne; Indarto, Indarto; Juffrie, Mohammad; Dharmastiti, Rini

doi:10.1063/1.4958474

Cited by 2 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vero cells, in addition to endothelial cells is the standard model that is commonly used in dengue infection. The infectivity of Vero cell line is higher than the endothelial cells and slightly lower than the monocytes [22]. Vero cells are highly susceptible to many viruses in humans and animals and its membrane proteins are responsible for virus entry.…”

Section: Introductionmentioning

confidence: 98%

Productive and non-productive phase of dengue virus infection in human umbilical cord blood: Egress Viral Particles on Vero cell line

Vats

Puc

et al. 2023

Preprint

View full text Add to dashboard Cite

Although dengue virus (DENV) can establish infection in hematopoietic stem progenitor cells (HSPCs), there is little information on dengue virus persistent infection in CD34+ and CD133+ cell surface glycoprotein of hematopoietic stem cells (HSCs). CD34 and CD133 also function as cell-cell adhesion factors which are present in umbilical cord blood (UCB). In this study, we attempted to establish a persistent infection model of DENV infection in UCB by infecting for a prolonged period of 30 days. Post-infection of DENV exhibited a productive and non-productive phase of DENV production. Using plaque assay, western blot, and confocal microscopy, we Show that CD133 and CD34 cells are target cells for DENV infection. Moreover, we show that DENV particles can be recovered from the non-productive phase of DENV infected CD34 and CD133 cells after co-incubation with Vero cells. We concluded that CD133 and CD34 retain their capacity to produce the infectious virus due to proliferation and their ability to repopulate, as deduced from BrdU proliferation assay and flow cytometry analysis using t-distributed stochastic neighbor embedding. In summary, the platform to co-culture infected primitive HSCs from its non-productive phase onto Vero cells will give new insight into understanding the DENV dynamics in cell-to-cell transmission and reactivation of the virus.

show abstract

Section: Introductionmentioning

confidence: 98%

Productive and non-productive phase of dengue virus infection in human umbilical cord blood: Egress Viral Particles on Vero cell line

Vats

Puc

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

The CD133 and CD34 cell types in human umbilical cord blood have the capacity to produce infectious dengue virus particles

Vats

Puc

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Although dengue virus (DENV) can establish infections in hematopoietic stem progenitor cells (HSPCs), there is little information on dengue virus persistent infection in CD34+ and CD133+ cell surface glycoproteins of hematopoietic stem cells (HSCs). CD34 and CD133 also function as cell–cell adhesion factors, which are present in umbilical cord blood (UCB). In this study, we sought to establish a persistent infection model of DENV infection in UCB using a prolonged period of infection lasting 30 days. Post-infection, the results exhibited a productive and non-productive phase of DENV production. Using a plaque assay, Western blot, and confocal microscopy, we demonstrated that CD133 and CD34 cells are target cells for DENV infection. Moreover, we showed that DENV particles can be recovered from the non-productive phase of DENV-infected CD34 and CD133 cells after co-incubation with Vero cells. We concluded that CD133 and CD34 retain their capacity to produce the infectious virus due to proliferation and their ability to repopulate, as deduced from a BrdU proliferation assay and flow cytometry analysis using t-distributed stochastic neighbor embedding. In summary, the platform to co-culture infected primitive HSCs from their non-productive phase onto Vero cells will give new insights into understanding the DENV dynamics in cell-to-cell transmission and reactivation of the virus.

show abstract

Effect of dengue virus infection on the permeability of vero cells line

Cited by 2 publications

References 14 publications

Productive and non-productive phase of dengue virus infection in human umbilical cord blood: Egress Viral Particles on Vero cell line

Productive and non-productive phase of dengue virus infection in human umbilical cord blood: Egress Viral Particles on Vero cell line

The CD133 and CD34 cell types in human umbilical cord blood have the capacity to produce infectious dengue virus particles

Contact Info

Product

Resources

About