Effect of dental treatments on salivary immunoglobulin A of children with and without dental caries: A comparative study

Priya, P R Geetha; Asokan, Sharath; Karthick, K; Reddy, N Venugopal; Rao, V Arun Prasad

doi:10.4103/0970-9290.118004

Cited by 9 publications

(7 citation statements)

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“…The sIgA provides immunity by inhibition of microbial adhesion, toxins, and enzyme neutralization and by reducing hydrophobicity in synergism with lactoferrin and lysozymes [21]. Studies conducted on children suggest increased susceptibility to dental caries due to deficiency of sIgA and increased expression during caries suggesting a protective role; however, there is contradiction on correlation between dental caries and sIgAs [22][23][24]. In addition, matrix metalloproteinases (MMPs) and cathepsins are associated with dental caries progression and pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

Differentially Expressed Salivary Proteins in Dental Caries Patients

Khan

Waheed

Khurshid

et al. 2021

BioMed Research International

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Dental caries is a multifactorial disease mainly caused by cariogenic bacteria commonly found in the oral cavity. Dental caries may cause demineralization of the tooth, cavitation, hypersensitivity, pulp inflammation, and even tooth loss if left untreated. Saliva secreted in the oral cavity can serve as a tool for identification of biomarkers for early detection of diseases. In the present study, differential expression of salivary proteins from 33 dental caries patients was compared with 10 control subjects. The unstimulated saliva was analyzed by 12% SDS-PAGE and two-dimensional gel electrophoresis. Gelatin and casein zymography was performed to check for protease activity. Also, salivary IgAs from both groups were compared by sandwich ELISA technique. Dental caries patient’s saliva showed decreased caseinolytic and increased gelatinolytic activity probably due to metalloproteases and cathepsins. Mean salivary levels of sIgA were also significantly higher ( p < 0.018 ) in dental caries saliva samples. The 2D electrophoresis profile of both the groups showed regions on gel with visually detectable alterations in protein expression. The present study is among the few initial studies in the locality for identification of protein differences in saliva from dental caries patients and has demonstrated a good potential to identify alterations. However, a large population-based analysis is required to validate these findings to be translated as a tool for indicative applications.

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Section: Introductionmentioning

confidence: 99%

Differentially Expressed Salivary Proteins in Dental Caries Patients

Khan

Waheed

Khurshid

et al. 2021

BioMed Research International

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“…Similar conclusions have been reached by other authors, who note higher levels of sIgA in children susceptible to dental caries. [7][8][9][10] Al Amoudi et al 11 observe a similar relationship in children and also in their mothers. Higher levels of secretory IgA in saliva can be associated with the immune response to large numbers of bacteria from the Streptococcus mutans group in carious cavities, which are sites of retention for food debris and bacteria.…”

Section: Discussionmentioning

confidence: 89%

“…Do podobnych wniosków doszli inni autorzy, którzy w swoich badaniach stwierdzili wyższy poziom S-IgA u dzieci podatnych na próchnicę. [7][8][9][10] Al Amoudi i wsp. 11 zaobserwowali podobną prawidłowość u dzieci oraz dodatkowo u ich matek.…”

Section: Dyskusjaunclassified

Evaluation of the level of secretory immunoglobulin A and salivary pH with regard to the intensity of dental caries in preschool children subjected to topical application of fluoride

Kowalik¹,

Kujawiak²,

Grzegorczyk³

et al. 2015

J Stoma

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Wprowadzenie. Ze względu na etiologiczny czynnik wspólny dla różnego przebiegu próchnicy, jakim są bakterie Streptococcus mutans, można przypuszczać, że to właśnie wydzielnicza IgA może być jednym z istotnych czynników podatności na próchnicę. Dzięki funkcji buforowej śliny możliwe jest zachowanie równowagi kwasowo-zasadowej warunkującej utrzymanie optymalnej wartości pH. Cel pracy. Celem pracy była ocena poziomów immunoglobuliny A i wartości pH w ślinie w aspekcie intensywności próchnicy u dzieci w wieku przedszkolnym, poddanych fluoryzacji kontaktowej. Materiał i metody. Materiał stanowiła ślina uzyskana od 121 dzieci w wieku 4-6 lat. Badanych podzielono na 3 grupy na podstawie wskaźnika puw, I grupa dzieci wolne od próchnicy (puw=0), II z wartością puw ≥ 1 i ≤ 2 oraz grupa III z intensywnością próchnicy puw ≥ 3. Ocenie podlegał Evaluation of the level of secretory immunoglobulin A and salivary pH with regard to the intensity of dental caries in preschool children subjected to topical application of fluoride* Ocena poziomów wydzielniczej immunoglobuliny A i wartości pH w ślinie w aspekcie intensywności próchnicy u dzieci w wieku przedszkolnym, poddanych fluoryzacji kontaktowej*

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“…Twenty-four studies reported s-IgA as outcome ( 14 – 17 , 27 , 31 , 43 , 52 , 61 – 63 , 68 , 75 , 77 , 79 , 80 , 86 , 88 , 90 , 94 , 99 , 102 , 105 , 107 ) ( Supplementary Table S4 ). Enzyme-linked immunosorbent assay (ELISA) was the method used in most of the studies (41.6%).…”

Section: Resultsmentioning

confidence: 99%

Comparison of oral cavity protein abundance among caries-free and caries-affected individuals—a systematic review and meta-analysis

Silveira,

Prato,

Pilati

et al. 2023

Front. Oral. Health

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ObjectiveSome salivary proteins seem to be differently abundant among caries-free (CF) and caries-affected (CA) individuals, but previous results are contradictory precluding that definitive conclusion be drawn. A pooled analysis of the available evidence may provide more robust data on identifying oral cavity protein patterns among CF and CA individuals. This systematic review and meta-analysis (PROSPERO CRD42021269079) aimed to compare the oral cavity protein abundance among caries-free and caries-affected individuals.MethodsThis study was conducted following PRISMA guidelines. PubMed, Embase, and Web of Science databases were systematically assessed (up to February 2023) to retrieve clinical studies written in English, German, or in Latin-based languages that compared the oral cavity protein abundance among CF and CA individuals. Data extraction and methodological quality assessment (NIH guidelines) were independently performed by two investigators. Qualitative synthesis was performed from all included studies and meta-analysis was performed using a random-effects model with inverse variance for studies that reported the concentration of proteins or enzymatic activity. Standardized mean difference (SMD) with respective 95% confidence interval (CI) were calculated for each outcome.ResultsA total of 90 studies (two cohort and 88 cross-sectional designs) of more than 6,000 participants were selected for data extraction, being the quality of evidence graded as “fair” for most of them. The oral cavity of CF individuals presented lower total protein concentration [SMD = 0.37 (95% CI: 0.07–0.68; 18 studies)], lower total antioxidant capacity [SMD = 1.29 (95% CI: 0.74–1.85); 17 studies], and lower carbonic anhydrase activity [SMD = 0.83 (95% CI: 0.58–1.09); three studies], whereas CA individuals presented lower carbonic anhydrase concentration [SMD = −0.66 (95% CI: −1.00 to −0.32); three studies], urease [SMD = −0.95 (IC 95%: −1.72 to −0.17); four studies], and arginine deiminase system [SMD = −2.07 (95% CI: −3.53 to −0.62); three studies] activities. Antimicrobial peptides, secretory immunoglobulin-A concentrations and alpha-amylase activity were similar among individuals.ConclusionDifferences on oral cavity protein abundance were observed among CF and CA individuals. These data indicate some protein patterns for the oral health and dental caries conditions. Even when statistically significant, some of the results were not very consistent. Cohort studies need to be conducted to validate these results.

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Effect of dental treatments on salivary immunoglobulin A of children with and without dental caries: A comparative study

Abstract: Mere quantitation of salivary IgA levels might have no reflection on the functional antibodies involved in caries process, and successful dental treatment alone does not alter the salivary IgA levels, suggesting a multifaceted approach to combat the cariogenic challenge.

Cited by 9 publications

References 0 publications

Differentially Expressed Salivary Proteins in Dental Caries Patients

Differentially Expressed Salivary Proteins in Dental Caries Patients

Evaluation of the level of secretory immunoglobulin A and salivary pH with regard to the intensity of dental caries in preschool children subjected to topical application of fluoride

Comparison of oral cavity protein abundance among caries-free and caries-affected individuals—a systematic review and meta-analysis

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