Effect of depolymerized sodium alginate on Salmonella Typhimurium infection in human enterocyte-like HT-29-Luc cells and BALB/c mice

Kuda, Takashi; Hirano, Shino; Yokota, Yasushi; Eda, Mika; Takahashi, Hiroki; Kimura, Bon

doi:10.1016/j.jff.2016.11.009

Cited by 9 publications

(3 citation statements)

References 31 publications

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“…As prebiotic, alginate is an indigestible food ingredient which beneficial for parasite organism activity, leading to increasing growth and nonspecific immune responses (Poongodi et al, 2012;El-Desouky et al, 2012;Peso-Echarri et al, 2012;Schleder et al, 2017), increasing lipid metabolism (Güroy et al, 2011), viruses resistance and resistance (Boonsri et al, 2017), increasing intestinal effectiveness (Michiels et al, 2012), and increasing the resistance of organisms to stress (Nath et a.l, 2012). In addition, alginate also potentially increases the abundance of bacteriides capillosus bacteria in the gut (Kuda et al, 2017) and has antiviral, antifungal and antibacterial activity (Niu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Immune response and growth performance of crayfish Cherax quadricarinatus fed with supplementary diet of synbiotic

Amrullah¹,

Wahidah²

2019

Jurnal Akuakultur Indonesia

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This study aimed to investigate the effectiveness of synbiotic (prebiotic alginate and probiotic bacteria Micrococcus spp) on the immune responses, growth performance, and bacterial resistance of freshwater crayfish. The experimental diets were formulated in three levels of synbiotic: the probiotic bacteria Micrococcus spp+100 mg/L alginate (Syn100), Micrococcus spp+200 mg/L alginate (Syn200), Micrococcus spp+300 mg/L alginate (Syn300), each level was mixed with tested feed. The basal diet (without synbiotics) served as the control diet. During 40 days of rearing, immune responses observations were conducted every 10 days, while the crayfish weight were recorded on day 40. Furthermore, the challenge test was performed against the pathogenic bacteria of A. hydrophila and reared for a week. The result showed that synbiotics treatment of Syn300 and Syn200 could improve the immune response and increased the growth rate (P<0.05). Both were also heavier than the Syn100 treatment. The highest resistance to A. hydrophila pathogenic bacteria by demonstrating a higher survival rate was Syn300 and followed by Syn200 (70.00 ± 0% and 73.33 ± 5.77%, respectively), compared to Syn100 (56.67 ± 3.33%) and the control (33.33 ± 3.33%). The synbiotic Micrococcus spp. combined with alginate potentially induced immune responses, increased growth performance, and improved bacterial pathogens resistance, making it an ideal synbiotic to be developed. Keywords: synbiotic, prebiotic, probiotic, Sargassum sp., alginate, brown algae ABSTRAK Penelitian ini bertujuan untuk menguji efektivitas penggunaan sinbiotik (prebiotik alginat dan probiotik Micrococcus spp) terhadap respons imun, performa pertumbuhan dan resistensi lobster air tawar. Tiga perlakuan sinbiotik yaitu Micrococcus spp+100 mg/L alginat (Syn100), Micrococcus spp+200 mg/L alginat (Syn200), Micrococcus spp+300 mg/L alginat (Syn300), masing-masing dicampurkan ke dalam pakan dan diberikan ke lobster. Pakan tanpa campuran prebiotik digunakan sebagai pakan kontrol. Selama 40 hari pemeliharaan, pengamatan respon imun dilakukan setiap 10 hari dan pada hari ke-40 pemeliharaan dilakukan pengukuran bobot lobster. Selanjutnya, lobster di uji tantang dengan bakteri A. hydrophila dan dipelihara kembali selama satu minggu. Hasil penelitian menunjukkan bahwa pemberian pakan yang mengandung sinbiotik Syn300 dan Syn200 dapat meningkatkan respon imun dan pertumbuhan (P<0.05) lebih tinggi dibandingkan dengan perlakuan Syn100, sementara bobot tubuh terendah didapat pada perlakuan kontrol. Resistensi lobster tertinggi terhadap bakteri A. hydrophila yang ditunjukkan dengan sintasan tertinggi didapatkan pada perlakuan Syn300 (70.00±0.00%) dan Syn200 (73.33±5.77%) dibandingkan dengan perlakuan Syn100 (56.67±3.33%) dan kontrol (33.33±3.33%). Penelitian ini membuktikan bahwa sinbiotik Micrococcus spp. yang dikombinasikan dengan alginat dapat menginduksi respons imun, meningkatkan performa pertumbuhan ,dan resistensi terhadap patogen sehingga merupakan sinbiotik yang ideal untuk dikembangkan. Kata kunci : sinbiotik, prebiotik, probiotik, Sargassum sp., alginat, alga coklat

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Section: Discussionmentioning

confidence: 99%

Immune response and growth performance of crayfish Cherax quadricarinatus fed with supplementary diet of synbiotic

Amrullah¹,

Wahidah²

2019

Jurnal Akuakultur Indonesia

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“…Alginate has also been studied in animal models. Firstly, alginate was supplemented in 18 five-week-old male BALB/c mice [ 68 ]. They were divided into three groups: the first one was a control group; the second was represented by a group with a supplementation of 2% of high density alginate (HD-NA); and the last one was a group with a supplementation of 2% of depolymerized alginate (DM-NA).…”

Section: Nondigestible Carbohydrates and Microbiota Modulationmentioning

confidence: 99%

Non-Digestible Carbohydrates: Green Extraction from Food By-Products and Assessment of Their Effect on Microbiota Modulation

Expósito-Almellón,

Duque-Soto,

López-Salas

et al. 2023

Nutrients

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The nature and composition of the waste produced by food industrial processing make its abundance and accumulation an environmental problem. Since these by-products may present a high potential for revalorization and may be used to obtain added-value compounds, the main goals of the technological advancements have been targeted at reducing the environmental impact and benefiting from the retrieval of active compounds with technological and health properties. Among the added-value substances, nondigestible carbohydrates have demonstrated promise. In addition to their well-known technological properties, they have been discovered to modify the gut microbiota and enhance immune function, including the stimulation of immune cells and the control of inflammatory reactions. Furthermore, the combination of these compounds with other substances such us phenols could improve their biological effect on different noncommunicable diseases through microbiota modulation. In order to gain insight into the implementation of this combined strategy, a broader focus concerning different aspects is needed. This review is focused on the optimized green and advanced extraction system applied to obtain added-value nondigestible carbohydrates, the combined administration with phenols and their beneficial effects on microbiota modulation intended for health and/or illness prevention, with particular emphasis on noncommunicable diseases. The isolation of nondigestible carbohydrates from by-products as well as in combination with other bioactive substances could provide an affordable and sustainable source of immunomodulatory chemicals.

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“…Interestingly, the potential use of alginate against pathogen infections are getting interest (Kuda et al, 2017). Different studies have explored the effects of alginate on the adhesion and invasion of Salmonella Typhimurium in human enterocyte-like HT-29-Luc cells and also, in BALB/c mice model (Bai et al, 2017), observing that supplementation of high and low concentrations of alginate could alleviate liver and spleen infection in mice and had immunomodulatory effects.…”

Section: Algae-derived Polysaccharidesmentioning

confidence: 99%

Potential benefits of high-added-value compounds from aquaculture and fish side streams on human gut microbiota

Wang

Zhou

Selma‐Royo

et al. 2021

Trends in Food Science & Technology

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Effect of depolymerized sodium alginate on Salmonella Typhimurium infection in human enterocyte-like HT-29-Luc cells and BALB/c mice

Cited by 9 publications

References 31 publications

Immune response and growth performance of crayfish Cherax quadricarinatus fed with supplementary diet of synbiotic

Immune response and growth performance of crayfish Cherax quadricarinatus fed with supplementary diet of synbiotic

Non-Digestible Carbohydrates: Green Extraction from Food By-Products and Assessment of Their Effect on Microbiota Modulation

Potential benefits of high-added-value compounds from aquaculture and fish side streams on human gut microbiota

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