Gaseous ozone at 13 parts per million (ppm) is applied repeatedly (10-30 cycles) to oxidize dry arrowroot starch. The ozonation increases carboxyl content of the starch by 0.13-0.52%. The starch granule surface becomes rougher and it exhibits a presence of fissure particularly at 30 cycles. Crystallinity decreases with the increase of ozonation cycle but the A-type pattern of the native starch remains unchanged. Swelling volume, solubility, water absorption capacity, and gel strength of the native starch increases following ozonation particularly at 30 cycles. Freeze-thaw stability is also augmented as indicated by a decrease in syneresis. The ozonation at 10, 20 and 30 cycles remarkably decreases the syneresis of native starch (5.24%) to 0.02%, 0.002% and 0.001% respectively. No effect of ozonation on pasting point is observed but an increase of values of the other pasting properties is noted. Regarding thermal properties, the ozonation increases T 0 of the arrowroot starch. The decrease in T, along with the increase in H of the ozonated starch, suggests that the ozonation leads to the formation of more homogenous and stronger crystallites. Overall, the effectiveness of gaseous ozonation at low concentration on modifying starch properties is dependent on the number of ozonation cycles.