Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

Jeong, Wonwoo; Kim, Min Kyeong; Kang, Hyun‐Wook

doi:10.1177/2041731421997091

Cited by 32 publications

(32 citation statements)

References 45 publications

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“…SDS and sodium deoxycholate (SDC) are often used for decellularization, 27,28 although these detergents lead to the loss of ECM components. 29 Milder methods are required to retain the induction ability of cell functions at a high level. In this study, we proposed that decellularization efficacy would be determined by (1) solubilization of cellular components, (2) dissociation of cell-cell adhesion,…”

Section: Possibility To Improve Decellularization Methods For Tissues...mentioning

confidence: 99%

Comparison of decellularization protocols for cultured cell‐derived extracellular matrix—Effects on decellularization efficacy, extracellular matrix retention, and cell functions

Hoshiba

Yunoki

2022

J Biomed Mater Res

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The in vitro reconstruction of the extracellular matrix (ECM) is required in tissue engineering and regenerative medicine because the ECM can regulate cell functions in vivo. For ECM reconstruction, a decellularization technique is used. ECM reconstructed by decellularization (dECM) is prepared from tissues/organs and cultured cells.Although decellularization methods have been optimized for tissue-/organ-derived dECM, the methods for cultured cell-derived dECM have not yet been optimized. Here, two physical (osmotic shocks) and five chemical decellularization methods are compared.The decellularization efficacies were changed according to the decellularization methods used. Among them, only the Triton X-100 and Tween 20 treatments could not decellularize completely. Additionally, when the efficacies were compared among different types of cells (monolayered cells with/without strong cell adhesion, multilayered cells), the efficacies were decreased for multilayered cells or cells with strong cell adhesion.Retained ECM contents tended to be greater in the dECM prepared by osmotic shocks than in those prepared by chemical methods. The contents impacted cell adhesion, shapes, growth and intracellular signal activation on the dECM. The comparison would be helpful for the optimization of decellularization methods for cultured cells, and it could also provide new insights into developing milder decellularization methods for tissues and organs.

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Section: Possibility To Improve Decellularization Methods For Tissues...mentioning

confidence: 99%

Comparison of decellularization protocols for cultured cell‐derived extracellular matrix—Effects on decellularization efficacy, extracellular matrix retention, and cell functions

Hoshiba

Yunoki

2022

J Biomed Mater Res

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“…In line with the abovementioned studies, Jeong and coworkers studied the decellularization efficiency of different types of detergents on porcine liver tissue, as well as their effect on the cytocompatibility, printability, and biochemical and mechanical properties of the resulting dECM derived bio-inks (Jeong et al, 2021). Sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC), Triton X-100 (TX), and Triton X-100 with ammonium hydroxide (TXA) were examined.…”

Section: Design Of Bio-inks For Tissue Engineering and Disease Modelingmentioning

confidence: 99%

Decellularized Tissues for Wound Healing: Towards Closing the Gap Between Scaffold Design and Effective Extracellular Matrix Remodeling

David

Guiza-Arguello

Arango-Rodríguez

et al. 2022

Front. Bioeng. Biotechnol.

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The absence or damage of a tissue is the main cause of most acute or chronic diseases and are one of the appealing challenges that novel therapeutic alternatives have, in order to recover lost functions through tissue regeneration. Chronic cutaneous lesions are the most frequent cause of wounds, being a massive area of regenerative medicine and tissue engineering to have efforts to develop new bioactive medical products that not only allow an appropriate and rapid healing, but also avoid severe complications such as bacterial infections. In tissue repair and regeneration processes, there are several overlapping stages that involve the synergy of cells, the extracellular matrix (ECM) and biomolecules, which coordinate processes of ECM remodeling as well as cell proliferation and differentiation. Although these three components play a crucial role in the wound healing process, the ECM has the function of acting as a biological platform to permit the correct interaction between them. In particular, ECM is a mixture of crosslinked proteins that contain bioactive domains that cells recognize in order to promote migration, proliferation and differentiation. Currently, tissue engineering has employed several synthetic polymers to design bioactive scaffolds to mimic the native ECM, by combining biopolymers with growth factors including collagen and fibrinogen. Among these, decellularized tissues have been proposed as an alternative for reconstructing cutaneous lesions since they maintain the complex protein conformation, providing the required functional domains for cell differentiation. In this review, we present an in-depth discussion of different natural matrixes recently employed for designing novel therapeutic alternatives for treating cutaneous injuries, and overview some future perspectives in this area.

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“…Often, chemical agents adopted for cells removal (as well as the number of decellularization cycles) can significantly alter the biological and mechanical properties of dECM thus affecting the effectiveness of the derived bioinks. 202 , 208 …”

Section: Synthetic and Natural Materials For Meniscal Scaffolds Printing Conditioning And Bioprintingmentioning

confidence: 99%

Meniscus regeneration by 3D printing technologies: Current advances and future perspectives

et al. 2022

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Meniscal tears are a frequent orthopedic injury commonly managed by conservative strategies to avoid osteoarthritis development descending from altered biomechanics. Among cutting-edge approaches in tissue engineering, 3D printing technologies are extremely promising guaranteeing for complex biomimetic architectures mimicking native tissues. Considering the anisotropic characteristics of the menisci, and the ability of printing over structural control, it descends the intriguing potential of such vanguard techniques to meet individual joints’ requirements within personalized medicine. This literature review provides a state-of-the-art on 3D printing for meniscus reconstruction. Experiences in printing materials/technologies, scaffold types, augmentation strategies, cellular conditioning have been compared/discussed; outcomes of pre-clinical studies allowed for further considerations. To date, translation to clinic of 3D printed meniscal devices is still a challenge: meniscus reconstruction is once again clear expression of how the integration of different expertise (e.g., anatomy, engineering, biomaterials science, cell biology, and medicine) is required to successfully address native tissues complexities.

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Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

Cited by 32 publications

References 45 publications

Comparison of decellularization protocols for cultured cell‐derived extracellular matrix—Effects on decellularization efficacy, extracellular matrix retention, and cell functions

Comparison of decellularization protocols for cultured cell‐derived extracellular matrix—Effects on decellularization efficacy, extracellular matrix retention, and cell functions

Decellularized Tissues for Wound Healing: Towards Closing the Gap Between Scaffold Design and Effective Extracellular Matrix Remodeling

Meniscus regeneration by 3D printing technologies: Current advances and future perspectives

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