Effect of Development and Aging on the Response of Canine Lymphocytes to Phytohemagglutinin

Gerber, Jay D.; Brown, Albert L.

doi:10.1128/iai.10.4.695-699.1974

Cited by 34 publications

(11 citation statements)

References 22 publications

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“…The distribution of lymphocyte subpopulations in normal adult canine lymphoid tissues is well characterised (Rabanal and others 1995) and such cells have recently been enumerated in the gastrointestinal tract of normal adult dogs (Kolbjornsen and others 1994, Jergens and others 1996, German and others 1999a. Although data are not available for pups of 16 to 20 weeks of age, the numbers of T cells observed in the tissues of these pups were below the level that JOURNAL OF could reasonably be attributed to an agerelated effect (Gerber andBrown 1974, Sellon andothers 1996) (Hart 1979, Hogenesch andFelsburg 1992). These observations correlate with the markedly low serum immunoglobulins in this case.…”

Section: Dog 12mentioning

confidence: 87%

Possible immunodeficiency in related rottweiler dogs

Day

1999

J of Small Animal Practice

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A litter of eight rottweiler pups is described in which two dogs died before the age of six months from systemic inflammatory disease, and two further pups developed inflammatory skin disease. All seven pups tested had a markedly low serum immunoglobulin A (IgA) concentration (< 0.1 mg/ml) and six of these dogs also had subnormal serum IgG (< 0.1 to 6.4 mg/ml). Tissues taken from three diseased pups were examined immunohistochemically using a number of lymphoid markers. Secondary lymphoid tissues had a paucity of CD3+ T lymphocytes, although T cells were found within some inflammatory foci. B-lymphocyte follicles were present within lymphoid tissues, but there were irregularities of plasma cell development and a lack of plasma cells of all classes within mucosal and cutaneous sites. Inflammatory lesions were dominated by macrophages expressing class II molecules of the major histocompatibility complex. Serum immunoglobulins were also investigated in eight related, clinically normal adult dogs. Five of these dogs, from two separate breeding lines, had subnormal serum IgA (< 0.1 to 0.15 mg/ml). The spectrum of disease within the affected litter may be consistent with an underlying inherited immunological defect, and the observed immunological abnormalities suggest a more complex disorder than simple IgA deficiency.

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Section: Dog 12mentioning

confidence: 87%

Possible immunodeficiency in related rottweiler dogs

Day

1999

J of Small Animal Practice

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“…Within each of the four groups of normal, gingivitis, mild to moderate and advanced periodontitis, the variability in PHA stimulation is evident. Several in vitro factors, such as duration of culturing (Douglas, Kamin & Fudcnberg 1969), as well as host parameters, such as aging (Gerber & Brown 1974) and disease state (Notkins, Mergenhagen & Howard 1970, Saiaman 1970, Kauffman et al 1974, may influence the responsiveness of T-lymphocytes to PHA. A marked increase of the peak response of PBL following stimulation with PHA during human experimental gingivitis has recently been associated with a shift of that peak response to lower PHA concentrations (Lang & Smith 1976).…”

Section: Lymphocyte Transformation In Patients With Periodontal Diseasementioning

confidence: 99%

“…This response may miss the peak response, making proper evaluation difficult. b) A significantly depressed response of PBL to mitogen (PHA) has been demonstrated with increasing age in the canine (Gerber & Brown 1974), as well as in humans (Weksler & Hutteroth 1974). Furthermore, Holm-Pedersen, Gaumer & Folke (1975) reported that elderly subjects would lack a response associated with gingival inflammation when their PBL were stimulated with lipopolysacharide from the cell walls of gram-negative organisms.…”

Section: Lymphocyte Transformation In Patients With Periodontal Diseasementioning

confidence: 99%

Lymphocyte blastogenesis to plaque antigens in human periodontal disease

Lang

Smith

1977

J of Periodontal Research

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Lymphocyte transformation in response to dental plaque antigens has been used to determine the association between cellular immune responses and the periodontal status in man. An in vitro system for lymphocyte transformation was used. Triplicate microcultures of peripheral blood lymphocytes were stimulated with four different concentrations of V. alcalescens, B. melaninogenicus, F. nucleatum, A. viscosus, A. naeslundii, S. sanguis and pooled plaque of human origin. Phytohemagglutinin served as a positive and saline as negative control. The uptake of 3H‐thymidine during blastogenesis was measured by liquid scintillation counting. Forty‐eight subjects divided into four equal groups of individuals with either normal gingivae, gingivitis, mild to moderate, or advanced periodontitis participated in the study. The subjects were all between 35 and 45 years of age. The peripheral blood lymphocytes of the normal subjects did not generally undergo blastogenesis with any of the organisms. However, when blastogenesis occured in other groups, the highest stimulation was found in the advanced periodontitis group. B. melaninogenicus stimulated blastogenesis only in the advanced group. A. viscosus and A. naeslundii stimulated lymphocytes to various degrees in all except the normal group. All the other antigens did not show any differences in stimulations between any of the groups.

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“…In vitro problems, including antigen dose-response relationships (Kiger, Wright & Creamer 1974, Lang c& Smith 1976) and the addition of serum to culture (Ivanyi & Lehner 1971b), can alter results. Changes in the host's CMI due to aging (Gerber & Brown 1974, Weksler & Hiitteroth 1974 can also invalidate findings from groups differing in age.…”

mentioning

confidence: 99%

Lymphocyte blastogenesis to plaque antigens in human periodontal disease

Smith

Lang

1977

J of Periodontal Research

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This study undertook to correlate lymphocyte transformation to human dental plaque antigens with clinical estimates of periodontal disease. Forty‐eight patients with periodontal conditions ranging from normal gingivae to severe periodontitis were examined clinically. Oral cleanliness was determined by the Plaque Index System (Silness & Löe 1964) and gingival health was assessed using the criteria of the Gingival Index System (Löe & Silness 1963). Pocket depth and loss of periodontal attachment from the cementoenamel junction (Ramfjord 1959, Glavind & Löe 1967) were also measured. Triplicate microcultures of peripheral blood lymphocytes were stimulated with four different concentrations of human plaque antigens. The uptake of 3H‐thymidine during blastogenesis was measured by liquid scintillation counting. There was no correlation between the stimulation by most of the isolated plaque antigens or pooled plaque and the periodontal conditions as determined by plaque index, gingival index, pocket depth and loss of attachment. However, there was a significant, although low, correlation between clinical parameters and the stimulation of peripheral blood lymphocytes by B. melaninogenicus and A. viscosus. Stimulation with B. melaninogenicus correlated more highly with pocket depth and loss of attachment, while stimulation with A. viscosus and A. naeslundii correlated more highly with plaque and gingivitis scores. The possible role of these organisms in the pathogenesis of periodontal disease involving cellular immunity was discussed as was the interpretation of data obtained in blastogenesis using peripheral blood lymphocytes and its relevance to the local phenomenon of delayed hypersensivity.

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Effect of Development and Aging on the Response of Canine Lymphocytes to Phytohemagglutinin

Cited by 34 publications

References 22 publications

Possible immunodeficiency in related rottweiler dogs

Possible immunodeficiency in related rottweiler dogs

Lymphocyte blastogenesis to plaque antigens in human periodontal disease

Lymphocyte blastogenesis to plaque antigens in human periodontal disease

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