Effect of Dexmedetomidine-Mediated Insulin-Like Growth Factor 2 (IGF2) Signal Pathway on Immune Function and Invasion and Migration of Cancer Cells in Rats with Ovarian Cancer

Abstract

Background The aim of this study was to explore the effect of dexmedetomidine (DEX)-mediated insulin-like growth factor 2 (IGF2) signal pathway on immune function and cancer cell invasion and migration in rats with ovarian cancer. Material/Methods Forty rats with ovarian cancer were divided into 4 groups: model group, and low dose (0.2 μg/kg/hour DEX), medium dose (1.0 μg/kg/hour DEX), and high dose (5.0 μg/kg/hour DEX) DEX groups. In addition, 10 Fischer344 rats were selected as a normal group. Human NUTU-19 poorly differentiated epithelial ovarian cancer cell line cells were divided into 4 groups: a blank group and low dose, medium dose, and high dose DEX NUTU-19 groups. Results Compared with the normal group, in the other groups the serum interleukin (IL)-2 and interferon gamma (INF-γ) levels, CD4 + and CD8 + percentages, CD4 + /CD8 + ratio, and transformation rate of splenic lymphocytes were decreased, and the serum tumor necrosis factor alpha (TNF-α) level, IGF2, insulin-like growth factor 1 receptor (IGF1R), insulin receptor substrate 1 (IRS1) mRNA, and protein expressions in ovarian tissue were increased (all P <0.05). Results in the DEX groups compared with model group were the opposite of those in the other groups compared with normal group (all P <0.05). Compared with the blank group, in the other groups the proliferation, invasion, and migration of ovarian cancer cells were reduced significantly (all P <0.05). Compared with the low dose DEX NUTU-19 group, in the high dose DEX NUTU-19 group the invasion and migration of ovarian cancer cells weakened significantly (both P <0.05). Conclusions A certain dose of DEX can effectively inhibit IGF2 signal pathway activation to improve the immune function of rats with ovarian cancer, inhibiting the invasion and migration of ovarian cancer cells.

Dexmedetomidine Alleviates Inflammatory Response and Oxidative Stress Injury of Vascular Smooth Muscle Cell via α2AR/PI3K/AKT Pathway in Intracranial Aneurysm

“…Number of migrated cells were measured using an inverted microscope (Leica). Six elds were randomly selected, and the average was calculated [34].…”

Dexmedetomidine alleviates inflammatory response and oxidative stress injury of vascular smooth muscle cell via α2AR/GSK-3β/MKP-1/NRF2 axis in intracranial aneurysm

“…Number of migrated cells were measured using an inverted microscope (Leica). Six fields were randomly selected, and the average was calculated [ 36 ].…”

Dexmedetomidine mediates microRNA-185 to suppress ovarian cancer growth via inhibiting the SOX9/Wnt/β-catenin signaling pathway

“…Besides, a previous study also suggested that DEX treatment could inhibit OC growth and the tumor weight of OS rats in vivo (Cai and Tang, 2017). Recently, Lei Hou et al found that DEX could improve the immune function of OC rats and inhibit the invasion and migration of OC cells via inactivating the insulin-like growth factor 2 signaling pathway (Tian and Hou, 2019). Meanwhile, in this present study, DEX also presented a dose-dependent promoting role in OC cell apoptosis, with increased expression of cleaved caspase 3 and Bax while declined expression of Bcl-2 shown after DEX treatment.…”