Effect of Dezocine on IL-12 and IL-10 secretion and lymphocyte activation by culturing dendritic cells from human umbilical cord blood

Feng, Chang; Feng, Man; Jiao, Ran; Liu, Dongyi; Jin, Yanwu; Zhao, Xin; Xiao, Ruixue

doi:10.1016/j.ejphar.2016.12.035

Cited by 16 publications

(13 citation statements)

References 30 publications

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“…of cytokines and chemokines such as IL-12 and IL-10 that are capable of increasing T cell activation (Feng et al, 2017). Our data showed the yields of TNF-α and IL-12 were increased in a dose-dependent manner and L-EPS could also induce the DCs to secrete high levels of IL-6 and IL-10.…”

Section: Discussionmentioning

confidence: 59%

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Xiu

Zhang

et al. 2018

Food and Agricultural Immunology

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Exopolysaccharides act as mediators of cross-talk between probiotics and the host. Here, we found that EPS derived from probiotic Lactobacillus casei WXD030 strain (L-EPS) could modulate immune responses in vitro and in vivo. L-EPS could significantly enhance the proliferation and phagocytic activity as well as induce the production of NO, TNF-α, IL-1β and IL-6 in RAW264.7 cells. Furthermore, L-EPS could induce the maturation of BMDCs. In addition, L-EPS could largely increase the titres of OVA-specific antibodies and markedly enhanced T cell proliferation. Notably, L-EPS also increased expression of IL-4 and INF-γ expression in CD4 + T cells. Consistently, when used as an adjuvant in vivo with the foot-and-mouth disease vaccine, L-EPS largely enhanced the FMDVspecific antibody production. Collectively, these results suggested that L-EPS derived from probiotic L. casei strain had adjuvant activity, which may be a safe and efficacious adjuvant candidate suitable for a wide spectrum of prophylactic and therapeutic vaccines.

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Section: Discussionmentioning

confidence: 59%

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Xiu

Zhang

et al. 2018

Food and Agricultural Immunology

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“…[ 21 ] In our study, we measured the norepinephrine and serotonin concentration in the plasma and found that the levels of norepinephrine and serotonin were increased with Dezocine pretreatment, which may have potential interactions with antinociceptive pathways. Besides that, Dezocine plays a role in immunity by regulating IL-12 and IL-10 secretion, and affecting lymphocyte activity in process of tissue injury, [ 22 ] which may also contribute to the analgesic effect. To our knowledge, this is the first clinical study that implicated norepinephrine and serotonin in Dezocine-induced postoperative pain modulation.…”

Section: Discussionmentioning

confidence: 99%

Effect of preemptive dezocine before general anesthesia on postoperative analgesia in patients undergoing laparoscopic cholecystectomy

Zhou¹,

Zhang²,

Sun³

et al. 2018

Medicine

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Dezocine is proposed as an adjunctive analgesic for postoperative pain control. This randomized, double-blind, controlled study aimed to investigate the effect of preoperative Dezocine therapy on postoperative pain following laparoscopic cholecystectomy as well as the underlying mechanisms.Eighty patients scheduled for laparoscopic cholecystectomy were randomly allocated into 2 groups as follows: patients in Group D received Dezocine 0.15 mg/kg before anesthesia induction and patients in Group S received same volume of saline. The pain intensity, sedation score, sufentanil-based patient-controlled analgesia (PCA) consumption were recorded for 24 hours after surgery. Plasma concentrations of norepinephrine and serotonin were also measured.During the first 24 hours after surgery, the patients in Group D experienced lower pain score assessed by numerical rating scale (NRS) at 3 hours (rest: P = .038; movement: P = .036), 6 hours (rest: P = .038; movement: P = .036), 12 hours (rest: P = .038; movement: P = .036), and 24 hours (rest: P = .038; movement: P = .036). Dezocine also decreased the sedation levels at 5 minutes (P = .031) after arrival at the PACU. Sufentanil-based PCA consumption in Group D was decreased when compared with Group S in the second to fourth phase after surgery (6–12 hours: P = .017; 12–18 hours: P = .003; 18–24 hours: P = .039). Plasma norepinephrine and serotonin concentrations were higher in the Group D at 24 hours after surgery (norepinephrine: P = .009, serotonin: P = .042). In addition, Group D showed less incidence of nausea/vomiting (P = .032) as well as a higher postoperative satisfaction score after surgery (P = .017).In conclusion, preemptive Dezocine administration is suggested to be useful for the management of postoperative pain in short-lasting surgery such as laparoscopic cholecystectomy.

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“…Hence, IL-12 is a promising immunotherapeutic agent that links innate and adaptive immunity (13). IL-12 has been observed to have antitumor effects in several preclinical and clinical studies (14)(15)(16). In the present study, monocytes were isolated and differentiated into mature DCs, which were then transfected with lentiviruses constructed to overexpress IL-12.…”

Section: Introductionmentioning

confidence: 93%

Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth

Yao

Zeng

et al. 2019

Oncol Rep

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The present study aimed to investigate the antitumor effects of an intratumoral injection of dendritic cells (DCs) overexpressing interleukin-12 (IL-12) on melanoma. DCs, isolated from mouse spleen, were gene-modified using an IL-12 overexpression vector. Melanoma B6 cells were injected into C57BL/6 mice to generate tumors. Thereafter, DCs overexpressing IL-12 were injected into the tumors, and tumor volume was subsequently measured. Pathological changes in tumor tissue were detected by hematoxylin and eosin staining. The expression of interleukin-4 (IL-4) and IL-12 in tumors was measured by enzyme-linked immunosorbent assay, real-time PCR and western blotting. DCs were successfully isolated and a lentivirus vector expressing IL-12 was constructed. After intratumoral injection of phosphate-buffered saline (control group), tumor cells exhibited malignant growth; whereas tumors injected with DCs (DC group) or DCs + empty vector (DC + vector group) exhibited a small amount of inflammatory cell infiltration and limited areas of tissue necrosis. In contrast, tumors injected with DCs overexpressing IL-12 (DC + IL-12 group) displayed severe tissue necrosis, loss of cell structure, and inflammatory cell infiltration. Compared with the control group, the tumor volumes were significantly lower in the DC, the DC + vector and the DC + IL-12 groups, while the expression of IL-12 and IL-4 in the tumors was significantly higher. Importantly, the most marked changes in tumor volume and IL-12 and IL-4 expression were in the DC + IL-12 group, which were significantly greater than those in tumors treated with unmodified DCs. Hence, intratumoral injection of DCs overexpressing IL-12 exerted strong antitumor effects in melanoma, and biotherapy with DCs overexpressing IL-12 is a potential treatment strategy for melanoma.

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Effect of Dezocine on IL-12 and IL-10 secretion and lymphocyte activation by culturing dendritic cells from human umbilical cord blood

Cited by 16 publications

References 30 publications

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Effect of preemptive dezocine before general anesthesia on postoperative analgesia in patients undergoing laparoscopic cholecystectomy

Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth

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