Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis

Sobarzo, Cristian Marcelo; Lustig, Livia; Ponzio, Roberto; Denduchis, Berta

doi:10.1016/j.reprotox.2006.02.004

Cited by 32 publications

(24 citation statements)

References 36 publications

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“…Specific DNA fragmentation at nucleosomal units is one of the most characteristic features of apoptosis [26] . Detection of DNA fragments in situ using the TUNEL assay [27] is undertaken to investigate active germ cell apoptosis induced by diabetes [11] and other stimulants [10,28] in the testis. This method was used in the measurement of apoptosis in several organs other than the testis [14][15][16]29] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Melatonin on Testicular Damage in Streptozotocin-Induced Diabetes Rats

Güneli¹,

Tugyan²,

Öztürk

et al. 2008

Eur Surg Res

163

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Background: It is well known that diabetes mellitus is associated with impairment of testicular function. In the present study, we aimed to demonstrate the effect of melatonin on testicular damage in male rats with streptozotocin (STZ)-induced diabetes. Methods: Male Wistar rats were divided into 4 groups: (1) control group, (2) melatonin-treated nondiabetic group, (3) diabetic group and (4) melatonin-treated diabetic group. Diabetes was induced by STZ injection. Melatonin was administered intraperitoneally at the dose of 10 mg/kg for 5 days. Testicular damage was examined by using hematoxylin and eosin staining and periodic acid-Schiff staining, and apoptosis was determined by terminal-deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL). Potential disorders associated with seminiferous tubular sperm formation were evaluated using the Johnsen score. Results: Diabetic rats showed a reduction in seminiferous tubule diameter, increased thickening of the basement membrane in seminiferous tubules and degenerated germ cells. TUNEL-positive cells were significantly more numerous in diabetic rats than in control rats. Melatonin significantly attenuated the diabetes-induced morphological changes and germ cell apoptosis in the diabetic rat testis. The number of polymorphonuclear leukocytes was significantly decreased in group 4 when compared to group 3. Conclusions: These results suggest that intraperitoneal administration of melatonin for 5 days is a potentially beneficial agent to reduce testicular damage in adult diabetic rats, probably by decreasing oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Effect of Melatonin on Testicular Damage in Streptozotocin-Induced Diabetes Rats

Güneli¹,

Tugyan²,

Öztürk

et al. 2008

Eur Surg Res

163

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“…It may also be mentionable in this context that it was reported in different studies that BTB components may act as early targets of environmental and reproductive toxicants mostly leading to testicular disorders (Defamie et al 2001, Sobarzo et al 2006, 2009, Li et al 2009a, Salian et al 2009, Pointis et al 2011.…”

Section: Sc Btb and Junctional Proteinsmentioning

confidence: 92%

Blood–testis barrier and Sertoli cell function: lessons from SCCx43KO mice

Gerber¹,

Heinrich²,

Brehm³

2016

Reproduction

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The gap junction protein connexin43 (CX43) plays a vital role in mammalian spermatogenesis by allowing for direct cytoplasmic communication between neighbouring testicular cells. In addition, different publications suggest that CX43 in Sertoli cells (SC) might be important for blood-testis barrier (BTB) formation and BTB homeostasis. Thus, through the use of the Cre-LoxP recombination system, a transgenic mouse line was developed in which only SC are deficient of the gap junction protein, alpha 1 (Gja1) gene. Gja1 codes for the protein CX43. This transgenic mouse line has been commonly defined as the SC specific CX43 knockout (SCCx43KO) mouse line. Within the seminiferous tubule, SC aid in spermatogenesis by nurturing germ cells and help them to proliferate and mature. Owing to the absence of CX43 within the SC, homozygous KO mice are infertile, have reduced testis size, and mainly exhibit spermatogenesis arrest at the level of spermatogonia, seminiferous tubules containing only SC (SC-only syndrome) and intratubular SC-clusters. Although the SC specific KO of CX43 does not seem to have an adverse effect on BTB integrity, CX43 influences BTB composition as the expression pattern of different BTB proteins (like OCCLUDIN, b-CATENIN, N-CADHERIN, and CLAUDIN11) is altered in mutant males. The supposed roles of CX43 in dynamic BTB regulation, BTB assembly and/or disassembly and its possible interaction with other junctional proteins composing this unique barrier are discussed. Data collectively indicate that CX43 might represent an important regulator of dynamic BTB formation, composition and function.Reproduction (2016) 151 R15-R27

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“…12,13) Recent studies focused on the mediation of the peroxisome proliferator-activated receptor (PPAR) subtypes as potential mediators of phthalate-induced testicular toxicity. 5) Although the mechanism of steroid biosynthesis pathway in the testis is known, recent studies indicate a close link between PPAR and other nuclear receptors in the testes.…”

Section: Introductionmentioning

confidence: 99%

Effects of Di(2-ethylhexyl) Phthalate on Regulation of Steroidogenesis or Spermatogenesis in Testes of Sprague-Dawley Rats

Lee

Kim

Choi

et al. 2009

JOURNAL OF HEALTH SCIENCE

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Di(2-ethylhexyl) phthalate (DEHP) used as a common plasticizer additive in the manufacture of plastics, such as polyvinyl chloride (PVC). This study examined the effect of DEHP on steroidogenesis or spermatogenesis in the testes of Sprague-Dawley male rats treated orally with 250, 500, 750 mg/kg over a 30-day period. The expression levels of the steroidogenic-or spermatogenic-related genes were analyzed in the testis using a reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. High doses of DEHP (500 and 750 mg/kg) significantly decreased the testicular sperm counts and daily sperm production (DSP). In addition, serum testosterone levels were significantly lower in the DEHP treatment groups than in the control. The mRNA levels of SR-B1, StAR, PBR and CYP17 increased in a dose-dependent manner. These increases were significant at 500 and 750 mg/kg. In the other hand, the mRNA levels of CYP19 decreased significantly in testes of rats exposed to DEHP 500 and 750 mg/kg. Dose-dependent decreases in Spag4 and LDHA mRNA in the testis were observed after DEHP exposure, while there was a significant decrease in thyroid hormone receptor (TR)α1 protein levels. High doses of DEHP significantly increased the expression of peroxisome proliferator-activated receptor (PPAR)-r and retinoid X receptor (RXR)-α protein but markedly decreased the expression of RXR-r. These results suggest that DEHP exposure can alter the expression of the spermatogenic-or steroidogenic-related genes resulting in a decrease in sperm production in the testis. This study is expected to be helpful in research examining the mechanisms for how DEHP reduces the expression pattern of the genes involved steroid hormone synthesis after chronic exposure to DEHP.

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Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis

Cited by 32 publications

References 36 publications

Effect of Melatonin on Testicular Damage in Streptozotocin-Induced Diabetes Rats

Effect of Melatonin on Testicular Damage in Streptozotocin-Induced Diabetes Rats

Blood–testis barrier and Sertoli cell function: lessons from SCCx43KO mice

Effects of Di(2-ethylhexyl) Phthalate on Regulation of Steroidogenesis or Spermatogenesis in Testes of Sprague-Dawley Rats

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