Effect of Dialysis on Erythrocyte Membrane of Chronically Hemodialyzed Patients

Abstract

The present work examines the role of uremia and the effect of dialysis treatment on red blood cells (RBCs) membrane properties of hemodialysis patients. The results showed that, the uremic patients had a lower values of erythrocyte deformability than that of healthy control subject. The median osmotic fragility (MOF) showed a significant increase in hemodialyzed patients than that for control group. The osmotic resistance to hemolysis was improved after dialysis. The solubilization process of the RBCs membrane showed that the detergent concentration needed to solubilize the RBCs membrane for uremic patient was much higher than that for control group. The abnormalities of the present results for RBCs membrane properties are mostly related to membrane fluidity, which are slightly improved after dialysis. Biochemical analysis showed a decreasing trend in RBCs count, urea nitrogen, creatinine, potassium,

Does Glucose in Dialysis Fluid Protect Erythrocytes in Patients with Chronic Renal Failure?

“…both glycated membrane proteins and F 2 -isoprostanes, can therefore contribute to the improvement in RSR to some extent. Ibrahim et al [21] found that the HD procedure increases the hemolytic resistance. It is probably caused by the removal of low molecular uremic toxins and the normalization of plasma osmolality.…”

“…It is probably caused by the removal of low molecular uremic toxins and the normalization of plasma osmolality. The changes occurring in RBC membranes during HD are associated with membrane fluidity which is slightly increased during the procedure [20,21,38] . In the RBC membranes of dialyzed patients, there is an increase in cholesterol levels, as well as a reduction in content of saturated fatty acids and an increase in content of unsaturated acids.…”

“…The erythrocyte osmotic fragility is a measurable expression of their susceptibility to hemolytic factors. It considerably depends on the cell membrane structure [15] , age (partially connected with modification of structure and metabolism during their lifespan) [19] , metabolism and other external factors moderating (enhancing or decreasing) the sensitivity of erythrocytes to hemolysis [20,21] . The biochemical test, which shows the hemolysis extent (but does not indicate its reason), is plasma extracellular hemoglobin concentration [22,23] .…”

Neutrophil Activation and Erythrocyte Membrane Protein Composition in Stage 5 Chronic Kidney Disease Patients

“…Studies performed in erythrocytes from stage 5 CKD patients, using cuprophane and polyacrylonitrile dialysis membranes, showed some changes in the membrane proteins, namely, a reduction in spectrin and band 3, and an isolated reduction in band 3, respectively (Sevilhano, 1990;Delmas-Beauvieux, 1995). Wu et al (Wu, 1998) and Ibrahim et al (2002) showed that stage 5 CKD patients presented a median osmotic fragility higher than the controls, and, after the haemodialysis procedure, that osmotic fragility decreased.…”

“…In literature, there are few reports about the effect of CKD and haemodialysis procedure in erythrocyte membrane protein composition (Matos, 1997;Wu, 1998;Ibrahim, 2002). Studies performed in erythrocytes from stage 5 CKD patients, using cuprophane and polyacrylonitrile dialysis membranes, showed some changes in the membrane proteins, namely, a reduction in spectrin and band 3, and an isolated reduction in band 3, respectively (Sevilhano, 1990;Delmas-Beauvieux, 1995).…”