Effect of Dibutyryl Cyclic AMP on<i>in Vitro</i>Rat Testis DNA, RNA and Protein Labeling

Hollinger, Mannfred A.; Hwang, F

doi:10.1210/endo-94-2-444

Cited by 9 publications

(2 citation statements)

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“…In the regulation of male reproductive function, cyclic nucleotides are involved in the reduction of spermatogonial division (Hollinger and Hwang 1974) and inhibition of spermiation (Gravis 1978), as well as in the epididimal sperm maturation (Sanborn et al 1980). They also play key roles in somatic cells of the testis, which directly regulate the microenvironment where germ cells differentiate, besides hormonally controlling testis function.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical localisation of PDE5 in Leydig and myoid cells of prepuberal and adult rat testis

Scipioni

Stefanini

Santone

et al. 2005

Histochem Cell Biol

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Expression of phosphodiesterase 5 (PDE5) in the rat testis at several pre and postnatal developmental stages was investigated by immunohistochemistry. The enzyme was localised in vascular smooth muscle cells, as well as in Leydig and peritubular cells. The latter were identified as myoid, based on their immunoreactivity to desmin and alpha-smooth muscle actin. The presence of PDE5 in myoid cells was confirmed by Western blot analysis and immunohistochemistry performed on highly purified cell fractions, obtained from 16-day-old rats. The expression of PDE5 in these somatic cells of rat testis is discussed in view of the roles played by cGMP signal transduction pathways in the mammalian male reproductive function.

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Section: Introductionmentioning

confidence: 99%

Immunohistochemical localisation of PDE5 in Leydig and myoid cells of prepuberal and adult rat testis

Scipioni

Stefanini

Santone

et al. 2005

Histochem Cell Biol

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“…At the level of the germ cells, they are thought to be involved in the control of sperm maturation in the epididymis (see Sanborn, Heindel & Robison, 1980, for review). Furthermore, the observation that high levels of cAMP cause a reduction in spermatogonial divisions (Hollinger & Hwang, 1974) and inhibition of spermiation (Gravis, 1978) suggests a participation of cyclic nucleotides in the control of male germ cell differentiation in the seminiferous tubules either acting directly within the germ cells or via the mediatory role of Sertoli cells. The hypothesis that cyclic nucleotides participate in the control of germ cell differentiation is further strengthened when it is considered that high cAMP concentration is required to prevent resumption of meiosis in amphibian and mammalian oocytes (Morrill, Zeigler & Kostellow, 1981 ; Schultz, Montgomery & Belanoff, 1983) and to prevent the onset of meiosis in yeast (Matsumoto, 1983).…”

Section: Introductionmentioning

confidence: 99%

Cyclic nucleotide phosphodiesterases in somatic and germ cells of mouse seminiferous tubules

Rossi¹,

Pezzotti²,

Conti³

et al. 1985

Reproduction

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Summary. The distribution of phosphodiesterase forms in somatic and germ cells, and their variations during testicular development and germ cell differentiation have been investigated. Seminiferous tubules from immature mice and Sertoli cells in culture possessed two enzyme activities which were comparable to forms described for different tissues and species: (a) a calcium\p=n-\calmodulin-dependentenzyme with high affinity for guanosine 3',5'-(cyclic)-monophosphate (cGMP), and (b) a calcium\p=n-\ calmodulin-independent enzyme with high affinity for adenosine 3' ,5' -(cyclic)\x=req-\ monophosphate (cAMP) the activity of which increased in cultured Sertoli cells after treatment with FSH or dibutyryl cAMP. Seminiferous tubules from adult animals and germ cells at the meiotic and post-meiotic stage of differentiation possessed two enzyme forms that could be distinguished from those present in somatic cells of the seminiferous tubules: (a) a calcium\p=n-\calmodulin-dependent form with high affinity for both cAMP and cGMP, similar to forms described in other tissues from different species, and (b) a calcium\p=n-\calmodulin-independentphosphodiesterase with high affinity for cAMP and present only in post-meiotic cells, previously identified also in germ cells of the rat.

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Coupled Events in the Early Biochemical Actions of FSH on the Sertoli Cells of the Testis

Means

Huckins

1974

Hormone Binding and Target Cell Activation in the Testis

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Effect of Dibutyryl Cyclic AMP onin VitroRat Testis DNA, RNA and Protein Labeling

Cited by 9 publications

References 0 publications

Immunohistochemical localisation of PDE5 in Leydig and myoid cells of prepuberal and adult rat testis

Immunohistochemical localisation of PDE5 in Leydig and myoid cells of prepuberal and adult rat testis

Cyclic nucleotide phosphodiesterases in somatic and germ cells of mouse seminiferous tubules

Coupled Events in the Early Biochemical Actions of FSH on the Sertoli Cells of the Testis

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