Effect of Diclofenac and Simvastatin on Bone Defect Healing—An In Vivo Animal Study

Karanikola, Theodora; Cheva, Angeliki; Sarafidou, Katia; Myronidou-Tzouveleki, Maria; Tsavdaridis, Ioannis; Kontonasaki, Eleana; Tsirlis, Anastasios

doi:10.3390/biomimetics7040143

Cited by 10 publications

(7 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding the Sim group, at week 2, the results showed better effects for bone formation compared to the Co group, which agrees with the results of Zhao et al [ 55 ]. However, after 4 weeks, the results of the study showed that there was no effect of Sim when compared to the Co group, disagreeing with the results of Karanikola et al [ 56 ]. The variance in the results is mostly attributed to the difference in the study model and experimental site.…”

Section: Discussioncontrasting

confidence: 83%

Chitosan Nanoparticle/Simvastatin for Experimental Maxillary Bony Defect Healing: A Histological and Histomorphometrical Study

Alsaeed,

Al-Ghaban

2023

Biomimetics

View full text Add to dashboard Cite

Biomaterials such as chitosan and simvastatin (Sim) have been introduced to accelerate the extensive and multicellular biological process of bone healing. The aim of this study was to evaluate the bone healing potential of chitosan and Sim, alone or combined. Forty-two male New Zealand rabbits were divided into three groups: chitosan nanoparticles (ChN), Sim and chitosan simvastatin nanoparticles (ChSimN). Two bony defects were created in the maxillary bone. The hole on the right side received one of the experimental materials, while the other side was assigned as the control and left to heal without any intervention. Bone specimens were collected at 2 and 4 weeks and then taken for histological and histomorphometrical analyses. The histological findings revealed that ChN possessed the highest number of osteoblasts and osteoclasts at weeks 2 and osteocytes after 4 weeks. There was a significant difference between the two healing periods regarding all bone parameters across all groups. ChN stood out as the only group that had a significant difference in the count of all bone cells between the two periods, thus having the best potential in promoting bone healing.

show abstract

Section: Discussioncontrasting

confidence: 83%

Chitosan Nanoparticle/Simvastatin for Experimental Maxillary Bony Defect Healing: A Histological and Histomorphometrical Study

Alsaeed,

Al-Ghaban

2023

Biomimetics

View full text Add to dashboard Cite

show abstract

“…Its known that the systemic administration of DCS has a negative effect on bone healing in animals [ 39 ]. As local medication, however, 5% IBP and 5% DCS associated with Ca(OH) 2 pastes have shown low cytotoxicity in a mouse pre-osteoblast cell line [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Ferrer-Luque

Solana

Aguado³

et al. 2023

Antibiotics

View full text Add to dashboard Cite

Persistent infections have become a challenge in dentistry because of growing antibiotic resistance. Nonsteroidal anti-inflammatory drugs (NSAIDs) appear to be a therapeutic alternative to control biofilm infection. The objective of this work is to evaluate the antimicrobial activity and cytotoxicity of sodium diclofenac (DCS), ibuprofen (IBP) and ibuprofen arginine (IBP-arginine) solutions against endodontic polymicrobial biofilms. Sterile radicular dentin blocks of 4 mm × 4 mm × 0.7 mm were used as substrate to grow biofilm. The dentin blocks were submerged into solutions for 5 min. The antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay and confocal laser scanning microscopy (CLSM). Fibroblasts 3T3-L1 (ECACC 86052701) were used to test the cytotoxicity of irrigating solutions. The antibiofilm effects determined by the ATP assay showed that 4% IBP-arginine solution exerted the highest antibiofilm activity, followed by 4% DCS and 4% IBP, with statistical differences among groups (p < 0.001). As for CLSM, 4% DCS and 4% IBP-arginine solutions gave the lowest viable cell percentages, without significant differences between them. Cytotoxicity results at 1/10 dilution were similar for all solutions. At 1/100 dilution, a 4% DCS solution obtained the lowest cell viability for both time periods assayed, 1 h and 24 h. The IBP-arginine group showed the highest cell viability at 24 h. In this preliminary study, in terms of antibiofilm activity and cytotoxicity, a mixed 4% IBP-arginine solution gave the most promising results. NSAID solutions could be recommendable drugs for endodontic disinfection procedures.

show abstract

“…A median sagittal incision was made along the top of the skull, parietal bones were exposed, and two circular calvarial bone defects, 1‒2 mm in thickness, were created on both sides of the sagittal suture, with a 6-mm-diameter trephine drill at 1500 rpm under saline irrigation to prevent excessive heating ( Figure 1 ). 40 According to the study of Porto et al 41 in 2012, this size of the defects is critical for the 15-day evaluation period. Then, the periosteum and skin were carefully sutured with 4-0 silk (skin) and 4-0 vicryl (periosteum).…”

Section: Methodsmentioning

confidence: 99%

“…Based on our pilot study, 40 we considered that the primary outcome (defect regeneration percentage) in the control group would be approximately 20 ± 10%. Based on this, to detect a difference of ± 15% between the groups, at least seven animals would be needed in each group to reach a power (1-β) of 0.80, with α = 0.05.…”

Section: Methodsmentioning

confidence: 99%

Effect of direct oral anticoagulant dabigatran on early bone healing: An experimental study in rats

Kyriakaki,

Karanikola,

Lillis

et al. 2023

J Adv Periodontol Implant Dent

Self Cite

View full text Add to dashboard Cite

Background. Dabigatran belongs to the new generation of direct oral anticoagulants (DOACs). Its advantages are oral administration and no need for international normalized ratio (INR) monitoring. Although its use has increased, its potential side effects on bone healing and remodeling have not been fully investigated. The present study aimed to evaluate the possible effects of dabigatran on early bone healing. Methods. Sixteen male Wistar rats were divided into two groups; in group A, 20-mg/kg dabigatran dose was administered orally daily for 15 days, while group B served as a control. Two circular bone defects (d=6 mm) were created on either side of the parietal bones. Two weeks after surgery and euthanasia of the animals, tissue samples (parietal bones that contained the defects) were harvested for histological and histomorphometric analysis. Statistical analysis was performed with a significance level of α=0.5. Results. No statistically significant differences were found between the two groups regarding the regenerated bone (21.9% vs. 16.3%, P=0.172) or the percentage of bone bridging (63.3% vs. 53.5%, P=0.401). Conclusion. Dabigatran did not affect bone regeneration, suggesting that it might be a safer drug compared to older anticoagulants known to lead to bone healing delay.

show abstract

Effect of Diclofenac and Simvastatin on Bone Defect Healing—An In Vivo Animal Study

Cited by 10 publications

References 43 publications

Chitosan Nanoparticle/Simvastatin for Experimental Maxillary Bony Defect Healing: A Histological and Histomorphometrical Study

Chitosan Nanoparticle/Simvastatin for Experimental Maxillary Bony Defect Healing: A Histological and Histomorphometrical Study

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Effect of direct oral anticoagulant dabigatran on early bone healing: An experimental study in rats

Contact Info

Product

Resources

About