Effect Of Diesel Fuel Pollution On The Lipid Composition Of Some Wide-Spread Black Sea Algae And Invertebrates

Nechev, Jordan; Khotimchenko, S. V.; Ivanova, Alexandra; Stefanov, Kamen; Dimitrova-Konaklieva, Stefka; Andreev, Stoitse; Попов, Симеон

doi:10.1515/znc-2002-3-401

Cited by 10 publications

(3 citation statements)

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“…The effect of the petroleum pollution and its derivatives on the marine microalgae is well studied. Nechev et al (2002) postulated that oil pollutants could penetrate at least partially into the cells, instead of covering only the outer surface of the marine organism. However, Wodzinski and Coyle (1974) showed that microorganisms utilize only water-soluble molecules of the oil that are dissolved in the aqueous phase.…”

Section: Introductionmentioning

confidence: 99%

Temporal alterations of Nannochloropsis salina (Eustigmatophyceae) grown under aqueous diesel fuel stress

et al. 2005

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The influence of an aqueous extract of diesel fuel was tested on growth of the marine eustigmatophyte Nannochloropsis (Monallantus) salina Hibberd. An increase in the concentration of the pollutant led to a decrease in growth rate as measured by optical density, with maximum effect observed (33% of control) at 100% aqueous pollutant. Spectrophotometric examination of cell viability (using Evan's blue dye) showed a significant negative effect of the diesel extract ( p ≤ 0.05, r = −0.92). Infrared spectra showed a slight change in the absorbance of contaminated compared with controlled cells. Proteome analysis (sodium dodecyl sulfate polyacrylamide gel electrophoresis -"SDS-PAGE") indicated that cell protein profiles depended on the pollutant concentration. Some of the resultant bands were characteristic to the pollutant concentration applied, indicating a distinct effect of the pollutant on the proteome structure. Iodine and toluidine blue dyes were applied using light microscopy to detect starch and mucilage, respectively. This indicated the presence of starch during all treatments, while the mucilage has been reduced. Transmission electron microscopy showed alterations to cell walls and membranes with different degrees of plasmolysis leading to a gradual increase in cell volume. However, the nucleus, the nucleolus and the pyrenoid remained unaffected. Similar results were obtained when the alga was cultured for 25 days in the 100% aqueous diesel extract indicating that long-term culture does not affect the degree of pollutant stress. Further, these cells recovered their normal appearance and characteristics within two days of being transferred to culture medium free of extract, indicating that N. salina shows a high tolerance to aqueous diesel fuel pollution.

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Section: Introductionmentioning

confidence: 99%

Temporal alterations of Nannochloropsis salina (Eustigmatophyceae) grown under aqueous diesel fuel stress

et al. 2005

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“…This significant increase in total lipids in oil-exposed gastropods could be a compensatory response to the stress inflicted by oil hydrocarbons. It is well known that the effect of oil spills on aquatic organisms is essentially caused by their exposure to organic hydrocarbons, which can be accumulated in the lipid-rich tissues of marine animals [75]. Interestingly, the predicted functional profiles of the associated bacterial community of P. ulvae also revealed a higher abundance of pathways related to both lipid and glycan biosynthesis in OnOi (Figure 7; Table S15).…”

Section: Biochemical Markers In the Gastropod Peringia Ulvaementioning

confidence: 92%

“…The reduced levels of LPO in oil-exposed mudsnails compared to non-exposed organisms are indicative of an absence and/or reduction of lipid peroxidation. It is well known that oil hydrocarbons can alter the content of fatty acids in invertebrates [75], thus contributing to a reduction in the levels of highly polyunsaturated fatty acids, which are very prone to oxidative stress and damage [76,77]. Accordingly, the low levels of LPO in oil-exposed gastropods compared to non-exposed organisms might be explained by a reduction in the polyunsaturated fatty acid content.…”

Section: Biochemical Markers In the Gastropod Peringia Ulvaementioning

confidence: 99%

Effects of Reduced Seawater pH and Oil Contamination on Bacterial Communities and Biochemical Markers of Estuarine Animal Hosts

Louvado,

Galhano,

Lima

et al. 2024

Environments

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Ecosystem functioning depends on complex interactions between microorganisms, hosts, and the environment. Changes in environmental conditions (e.g., ocean acidification) in combination with anthropogenic pollution have been shown to affect the composition and function of free-living microbial communities, but little is known about the effects these stressors on host-associated communities. This study aims to characterize the response of host-associated bacterial communities of the bottom-dwelling polychaete Hediste diversicolor and the epibenthic gastropod Peringia ulvae to oil contamination and reduced seawater pH. The independent and interactive effects of both stressors were simulated under controlled conditions. The response of host-associated bacterial communities was assessed using the high-throughput sequencing of the 16S rRNA gene and several biochemical markers related to host metabolic pathways, e.g., neurotransmission, anaerobic metabolism, biotransformation, oxidative stress, and energy consumption. In H. diversicolor, reduced seawater pH was associated with a high relative abundance of Cyanobacteria, while in P. ulvae oil contamination was associated with a reduction in the relative abundance of Chitinophagales. In P. ulvae, enrichment with oil hydrocarbon-degrading bacteria suggests a possible role of these organisms in the dispersion of oil hydrocarbon degraders. Furthermore, oil supplementation shifted some specific biochemical markers of gastropods related to oxidative stress and energy consumption, which suggests host stress. In general, the bacterial communities and biochemical markers of the gastropod were more affected by stressors than those of the polychaete. Overall, this study contributes to a better understanding of the response of host-associated bacterial communities of benthic macrofauna to anthropogenic contamination and environmental change.

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Oyster's cells regulatory volume decrease: A new tool for evaluating the toxicity of low concentration hydrocarbons in marine waters

Naceur¹,

Maxime²,

Mansour³

et al. 2016

Ecotoxicology and Environmental Safety

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Effect Of Diesel Fuel Pollution On The Lipid Composition Of Some Wide-Spread Black Sea Algae And Invertebrates

Cited by 10 publications

References 1 publication

Temporal alterations of Nannochloropsis salina (Eustigmatophyceae) grown under aqueous diesel fuel stress

Temporal alterations of Nannochloropsis salina (Eustigmatophyceae) grown under aqueous diesel fuel stress

Effects of Reduced Seawater pH and Oil Contamination on Bacterial Communities and Biochemical Markers of Estuarine Animal Hosts

Oyster's cells regulatory volume decrease: A new tool for evaluating the toxicity of low concentration hydrocarbons in marine waters

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