Effect of diet and exercise intervention on the growth of prostate epithelial cells

Abstract

Epidemiological studies suggest a positive association between nutrient intake, hyperinsulinemia and risk of Benign prostatic hyperplasis (BPH). This study tests the hypothesis that a low-fat, highfiber diet and daily exercise would lower serum insulin and reduce the growth of serum-stimulated primary prostate epithelial cells in culture. Serum samples were obtained from eight overweight men before and after the Pritikin residential, 2-week diet and exercise intervention and from seven men who were long-term followers of the low-fat, high-fiber diet and regular exercise lifestyle. The serum was used to stimulate primary prostate epithelial cells in culture. Growth was measured after 48 and 96 h and apoptosis after 96 h. At 48 h there was no significant difference in growth within the Pre, 2-week or Long-Term groups. At 96 h growth was significantly reduced in the 2-week (13%) and in the Long-Term (14%) groups compared to the Pre data. At 96 h, apoptosis was not significantly different among the three groups. Fasting insulin was reduced by 30% in the 2-week group and by 52% in the Long-Term group compared to the Pre data. Testosterone was unchanged in the 2-week group. The results of this study indicate that a low-fat, high-fiber diet and daily exercise lowers insulin and reduces growth of prostate primary epithelial cells and suggests that lifestyle may be an important factor in the development or progression of BPH. Future prospective trials should address the effects of this lifestyle modification on BPH symptomatology and progression.

Studying the effect of type 2 diabetes mellitus on prostate-related parameters: A prospective single institutional study

“…In fact, both type 2 DM and BPH seem to share similar epidemiological features, possibly related to aging and diet 14 . Barnard et al 15 connected the reduction of growth of stem epithelial prostate cells with the reduction of insulin. Other possible mechanisms have been proposed to associate the development of BPH with type 2 DM, such as the increase in peripheral sympathetic nerve tone and activity of the autonomic nervous system caused by hyperinsulinemia, 16 and hypoxia caused by DM-induced vascular damage 17 …”

Enhanced expression of SOS1 is detected in prostate cancer epithelial cells from African-American men

“…Briefly, minced pieces of tissues were distributed to several collagen-coated cell culture dishes with keratinocyte serum-free medium (K-SFM) (Life Technologies, Inc., Gaithersburg, MD) supplemented with 5% fetal bovine serum. The serum was used to stimulate primary prostate epithelial cells in culture (27). Tissue explants were grown for three weeks until reaching confluence.…”

Wpływ nawyków żywieniowych na stężenie wybranych hormonów, parametrów biochemicznych i antropometrycznych u pacjentów z łagodnym rozrostem prostaty – badania wstępne

“…W literaturze opisana jest rola nawyków żywieniowych na rozwój raka prostaty [6,7,8] oraz BPH [4,5]. Nieliczne są publikacje określające związek diety ze stężeniem hormonów wpływających na etiologię BPH, natomiast wnioski z tych badań są często kontrowersyjne [5,9,10].…”

“…W badaniach własnych nie wykazano związku między rodzajem spożywanego pokarmu a stężeniem TT. Również inni autorzy [9] nie stwierdzili różnicy w stęże-niu testosteronu w zależności od stosowanej diety podczas 2-tygodniowej obserwacji.…”