Effect of Diet on Mucin Kinetics and Composition: Nutrition and Health Implications

Montagne, L.; Piel, C.; Lallès, Jean Paul

doi:10.1301/nr.2004.mar.105-114

Cited by 44 publications

(55 citation statements)

References 48 publications

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“…The predicted predominant types of mucin in the ileal effluent were gastric and small intestinal mucin. The concentration of digesta mucin detected here was within the range of other published values (32,33) and represented w13% of the digesta dry matter. Therefore, mucin is the single most abundant, truly endogenous component in the terminal ileal digesta, and any dietary factors (eg, fiber and lectins) or physiologic conditions (eg, inflammatory bowel disease) that increase mucin secretion can be expected to have a significant effect on endogenous nitrogen losses at the terminal ileum.…”

Section: Discussionsupporting

confidence: 88%

Endogenous proteins in terminal ileal digesta of adult subjects fed a casein-based diet

Miner-Williams¹,

Deglaire²,

Bénamouzig³

et al. 2012

The American Journal of Clinical Nutrition

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Section: Discussionsupporting

confidence: 88%

Endogenous proteins in terminal ileal digesta of adult subjects fed a casein-based diet

Miner-Williams¹,

Deglaire²,

Bénamouzig³

et al. 2012

The American Journal of Clinical Nutrition

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“…A lower number of GC containing acidic mucins in the colonic crypts in pigs fed 6.3 g as compared to 5.1 g SID Thr/kg may suggest a negative effect of the potentially high‐Thr level on acidic mucin production and, in consequence, on mucus layer thickness. The mucus layer plays an important role in protecting the gut against excessive proliferation of pathogenic bacteria and bacterial infections (Montagne, Piel, & Lallès, ). However, in our study, changes in GC morphology due to an increase of SID Thr from 5.1 to 6.3 g/kg were not accompanied by alternations of mucus layer thickness and the potentially negative effect of the high‐Thr level on the mucus layer was not observed.…”

Section: Discussionmentioning

confidence: 99%

Goblet cells and mucus layer in the gut of young pigs: Response to dietary contents of threonine and non‐essential amino acids

Święch

Tuśnio

Barszcz

et al. 2019

Animal Physiology Nutrition

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Mucins secreted by goblet cells (GC) are the major components of mucus layer coating and protecting gut epithelium. The study aimed at determining the effect of non‐essential amino acids (NEAA) and threonine (Thr) levels on GC number and mucus layer thickness measured in different parts of the gut. A two‐factorial experiment was conducted on 72 pigs (initial BW 12.5 kg) using as factors: Thr level (5.1, 5.7, 6.3 and 6.9 g standardized ileal digestible [SID] Thr/kg) and wheat gluten (WG) level used as a source of NEAA (20.4, 40.4 and 60.4 g WG protein in WG20, WG40 and WG60 diets respectively). All diets covered the requirement for essential AA, except for Thr, and they were fed to six pigs for 20 days. Thr level affected only the count of GC containing acidic mucins in the proximal colon, which was higher in pigs fed a diet with 5.1 g SID Thr/kg in comparison with diet containing 6.3 g SID Thr/kg. In the villi, WG40 and/or WG60 increased the GC number containing acidic mucins and lowered that with neutral mucins in the middle jejunum. In the crypts, higher WG levels decreased the GC number with acidic mucins in the duodenum, ileum and proximal colon and increased that with neutral and mixed mucins in the duodenum and ileum. The mucus layer was thicker in the proximal and middle jejunum in WG20 pigs, whereas in the proximal colon it was thicker in WG60 pigs. The lowest GC count was found in the middle jejunum and the highest in the duodenum and proximal colon. The mucus layer was the thinnest in the proximal and middle jejunum and the thickest in the ileum. The results did not show the negative effect of a moderate Thr deficiency and the positive effect of non‐essential AA supplementation on GC functions.

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“…Sialomucins were less abundant in the distal intestinal regions of the four bat species in the present study and is in agreement with other studies (Boonzaier et al, ; Sheahan & Jervis, ). Sialomucins are less mature than sulphomucins, rendering sialomucins more vulnerable to bacterial degradation of the large intestine (Montagne, Piel, & Lalles, ). Structurally, sialomucins are therefore best suited to the lighter bacterial load typically found in the small intestine (Filipe & Fenger, ; Meslin, Fontaine, & Andrieux, ).…”

Section: Discussionmentioning

confidence: 99%

A comparative morphological and histological study of the gastrointestinal tract of four insectivorous bat species: Asellia tridens, Chaerephon pumilus, Nycteris thebaica, Rhinopoma hardwickii

Aylward

Beselaar

Alagaili

et al. 2019

Journal of Morphology

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Various studies address the morphology of the gastrointestinal tracts (GITs) of insectivorous bat species. However, detailed morphometric studies including mucin histochemistry are scarce. This study compares various GIT measurements as well as the quantification of intestinal mucin secreting cells in four insectivorous bat species representing four different families of Chiroptera. Alcian blue/Periodic acid Schiff's stain was used to differentiate between acid and neutral mucin‐secreting cells while the Aldehyde fuchsin/Alcian blue stain further differentiated between two acid mucins, namely sialo‐, and sulphomucins. The number of cells was quantified and statistically analysed. All species had a simple GIT morphology represented by a simple, completely glandular stomach and the absence of a cecum. The exception was R. hardwickii, where a small cecum was observed which had histological mucosal features of both the small and large intestine. In R.hardwickii, distal to the cecum, typical colonic mucosal features such as the absence of villi and an abundance of goblet cells were observed. In all four species, the total number of goblet cells increased from the proximal to the distal intestinal regions. Mixed (acid and neutral) mucins dominated the entire GIT of all species. Neutral mucin‐secreting cells were observed in the gastric pylorus and proximal intestinal regions in all species. Brunner's glands stained positive for neutral mucins. Exclusively acid mucin‐secreting cells were seen in the distal intestinal regions of all species except N. thebaica. Sulphomucin‐secreting cells were the most prominent acid mucin cell‐type towards the distal intestine. The distribution of different mucin secreting cells indirectly provides information regarding the quality of the intestinal biofilm in the species studied.

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Effect of Diet on Mucin Kinetics and Composition: Nutrition and Health Implications

Cited by 44 publications

References 48 publications

Endogenous proteins in terminal ileal digesta of adult subjects fed a casein-based diet

Endogenous proteins in terminal ileal digesta of adult subjects fed a casein-based diet

Goblet cells and mucus layer in the gut of young pigs: Response to dietary contents of threonine and non‐essential amino acids

A comparative morphological and histological study of the gastrointestinal tract of four insectivorous bat species: Asellia tridens, Chaerephon pumilus, Nycteris thebaica, Rhinopoma hardwickii

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