Effect of dietary astaxanthin on reproductive performance, egg quality and larvae of clowfish <i>Amphiprion ocellaris</i> (Cuvier, 1830)

Abstract

This study was designed to evaluate the effects of astaxanthin in broodfish diets on reproductive performance, egg quality and larvae quality parameters of clownfish (Amphirion ocellaris). Five treatments were tested with 5 levels of astaxanthin (Carophyll Pink 10% CWS) of 0, 50, 100, 150 and 200 mg/kg added to the feed. Each treatment was repeated in triplicate and the supplemental feeding trials were arranged for 13 months. The results showed that there were significant differences in hatching rate of egg, malformed rate and survival rate of larvae in 3 days post-hatch (p&lt;0.05) among the feeding trials of astaxanthin supplements. The highest hatching rate of egg and survival rate and the lowest malformed rate of larvae were observed in the treatment that was supplemented with astaxanthin 150 mg/kg feed, respectively 92.14 %; 93.57 % and 0.55 %. However, the astaxanthin supplementary diets did not affect the re-maturation and spawning period, spawning frequency, fecundity, egg diameter and larval size of nemo fish among the treatments. The results also suggested that astaxanthin requirement for clownfish broodstock to improve reproductive performance was 150 mg/kg feed.

Recent progress in practical applications of a potential carotenoid astaxanthin in aquaculture industry: a review

“…In addition, the diameter and the number of fertilized eggs in the goldfish Carassius auratus were greater in groups fed 150 mg kg −1 of astaxanthin for four months, which correlated to higher egg survival rates in the incubation period (Tizkar et al 2013 ). Recent investigation on the effects of astaxanthin supplementary diets (150 mg kg −1 feed) on egg quality and larvae quality parameters of clownfish ( Amphiprion ocellaris ) has observed a higher hatching rate of egg and survival rate with a lower malformed rate of larvae (Hue et al 2020 ). It has been also claimed that in crustaceans, carotenoid supplementation was a vital process during the reproductive cycle (Liñán-Cabello et al 2003 ; Liñán-Cabello and Paniagua-Michel 2004 ).…”

Captivating Colors, Crucial Roles: Astaxanthin’s Antioxidant Impact on Fish Oxidative Stress and Reproductive Performance

“…The profound implications of astaxanthin extend to the realm of larval survival and early life-stage performance, an area that has garnered substantial scientific attention [144]. The survival of larval organisms holds pivotal importance, exerting notable influence on recruitment dynamics and population sustainability across diverse fish species [145].…”