Effect of dietary cholesterol on mevalonate metabolism by sterol and nonsterol pathways

Aguilera, J.A.; Linares, Ana María; Arce, V.; Garcia-Peregrin, E.

doi:10.1016/0006-291x(84)91182-3

Cited by 24 publications

(5 citation statements)

References 17 publications

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“…We have also used chicks previously to study lipid metabolism after cholesterol administration (30)(31)(32)(33). The aim of this study was to develop a cell-culture avian model to look into the effect of a cholesterol diet on the transformation of SMC in vivo.…”

mentioning

confidence: 99%

“…Avian models were used in trials of atherosclerosis, and, in fact, cholesterol feeding has been described elsewhere as producing atherosclerotic lesions in fowl species (26)(27)(28)(29). We have also used chicks previously to study lipid metabolism after cholesterol administration (30)(31)(32)(33). The aim of this study was to develop a cell-culture avian model to look into the effect of a cholesterol diet on the transformation of SMC in vivo.…”

mentioning

confidence: 99%

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Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding

Carazo¹,

Alejandre²,

Díaz³

et al. 1998

Lipids

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We have developed cultures of smooth muscle cells (SMC) isolated from arterial hypercholesterolemic chicks (cholesterol-SMC). These cultures are suitable for the study at the molecular level of the changes in arterial SMC induced by a cholesterol diet. By using a strong dose of cholesterol (5%) for 10 d, we obtained very proliferative SMC which became foam cells after 30 d in culture. On the other hand, SMC cultures isolated from control-fed chicks had a lower growth rate than the SMC ones under the same culture conditions. DNA synthesis was fourfold greater in cholesterol-SMC than in control-SMC cultures. Intracellular cholesterol concentrations were the same in both cholesterol and control SMC during the first 14 d of culture but afterward increased in differing ways: after 20 d of culture the cholesterol-SMC increased their cholesterol content to double the control. We give here the results obtained from transmission electron microscopy, lipid analysis, proliferation studies, DNA, RNA and protein synthesis, and then discuss their implications.

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confidence: 99%

mentioning

confidence: 99%

Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding

Carazo¹,

Alejandre²,

Díaz³

et al. 1998

Lipids

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“…In fact, dietary cholesterol supplementation to the newborn chick after 10 to 15 days produced a near 10-fold increase in the hepatic esterified cholesterol, whereas no significant variations were found in free cholesterol. The same dietary manipulation induced a significant inhibition not only of HMG-CoA reductase activity 40 but also of mevalonate incorporation into total nonsaponifiable lipids, 17,35 as well as in mevalonate 5-pyrophosphate decarboxylase activity. 44 A fraction of lanosterol derivatives and cholesterol precursors accumulated after cholesterol feeding may be responsible for the HMG-CoA reductase inhibition 45,46 as well as for the decrease of cholesterol synthesis from mevalonate.…”

Section: Discussionmentioning

confidence: 94%

“…16 Likewise, cholesterol content in neonatal chick liver sharply decreases during the first days after hatching. 17 These rapid changes in cholesterol content in different tissues render the newborn chick an interesting model for the study of cholesterol metabolism throughout postnatal development.…”

Section: Introductionmentioning

confidence: 99%

Research Communications

1988

afr asian stud

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“…Young chicks were used as experimental animals because of their high sensitivity to dietary changes (1,8,18). The rapid responses of VLD L to saturated fatty acids (6) and n-3 PUFA (4,5,7) in the chick as well as the rapid changes induced by a cholesterol-enriched diet in chick cultured smooth muscle cells (3) corroborate its suitability as a model of studying the development of atherogenesis.…”

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confidence: 99%

Effects of dietary fish oil on the fatty acid composition of the main lipid classes of chick plasma lipoproteins

Castillo

Amalik

Garcı́a-Fuentes

et al. 2002

J. Physiol. Biochem.

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We have studied the effects of diet supplementation with 10% fish oil on fatty acid composition of the main lipid classes of chick plasma lipoproteins bearing in mind the relationship between platelet aggregation and eicosanoid production from arachidonic acid. Fish oil drastically increased the percentages of 20:5 n-3 and 22:6 n-3 acids in the high density lipoprotein lipids. The 20:5/22:6 ratio increased in triacylglycerol fraction whereas in phospholipids and cholesterol esters both 20:5 and 22:6 acids increased in a similar proportion. The percentage of arachidonic acid was higher in phospholipids than in the other lipid classes from this lipoprotein fraction and was significantly reduced by fish oil feeding. Linoleic acid, which was the most abundant fatty acid in cholesterol esters, strongly decreased after fish oil consumption. Changes induced in low- and very low density lipoproteins were similar to that observed in the high density lipoproteins. However, in the very low density lipoproteins, the 20:5/22:6 ratio was not increased in triacylglycerols, in contrast to that found in the high- and low density fractions. Our results suggest that decreases observed by fish oil feeding in the percentages of arachidonic acid in phospholipids and linoleic acid in cholesterol esters in the three lipoprotein fractions may be of importance to explain some pharmacological effects of n-3 PUFA with regard to vascular diseases.

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Effect of dietary cholesterol on mevalonate metabolism by sterol and nonsterol pathways

Cited by 24 publications

References 17 publications

Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding

Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding

Research Communications

Effects of dietary fish oil on the fatty acid composition of the main lipid classes of chick plasma lipoproteins

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