Effect of dietary fatty acid composition on the growth of the tiger puffer Takifugu rubripes

Abstract

Effects of dietary fatty acid composition on the growth of the tiger puffer Takifugu rubripes were examined. Eight experimental diets were formulated with fish meal and casein as the major ingredients, providing 45.0-48.2% crude protein. Pollack and squid liver oils were used for the control diet while experimental diets contained three levels of EPA-DHA concentrated (C-HUFA) oils, soybean oil, linseed oil, and combinations of them, providing 0.5-5.5% n-3 HUFA and 5.0-11.1% crude lipid. Triplicate groups of fish with a mean body weight of 18.7 g per dietary treatment were fed the diets twice daily to apparent satiation, 6 days per week for 8 weeks. The final body weight and weight gain of the fish fed the soybean oil diet without C-HUFA oils were significantly lower than those fed the control diet. Both parameters were not statistically different among the other dietary groups, although the values obtained for the linseed oil diet without C-HUFA oils were lower. Similar trends were seen for SGR and feed efficiency. However, significantly lower PER values compared to the control were observed for the lower C-HUFA and the vegetable oil without C-HUFA oil groups. These results indicate that tiger puffer can be produced successfully with diets containing more than 1.5% n-3 HUFA.

Effects of different dietary lipid levels on intestinal mucosal barrier and microbial community of juvenile tiger puffer Takifugu rubripes

“…Three diets were formulated (Table 1). A diet with a suitable lipid level (90.3 g/kg dry matter) for tiger puffer (Kikuchi et al, 2011) was used as the control diet (C-SL, control-suitable lipid). By adding fish…”

Effects of dietary n‐6 polyunsaturated fatty acids on growth performance, body composition, haematological parameters and hepatic physiology of juvenile tiger puffer (Takifugu rubripes)

“…However, the information about fatty acid requirement in this fish was very limited. Only one relevant previous study has been reported, which showed that docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), two main n‐3 polyunsaturated fatty acids (PUFAs), were essential in maintaining normal growth performance of tiger puffer (Kikuchi et al, ). However, the functions of n‐6 PUFA in diets for tiger puffer were still unknown.…”

“…However, the functions of n‐6 PUFA in diets for tiger puffer were still unknown. In the study of Kikuchi et al (), it has been indicated that the use of soybean oil, which is characterized by high linoleic acid (LA) content, as sole lipid source led to reduced growth performance of tiger puffer. However, no information has been available about the efficacy of arachidonic acid (ARA) in diets for tiger puffer which do not seem to possess the desaturase genes (according to the existing genome sequence) required for biosynthesis of ARA, EPA and DHA from LA and linolenic acid (LNA).…”

Effects of Dietary Lipid Sources and Essential Fatty Acids on the Growth and Body Composition of the Juvenile River Puffer Fish Takifugu obscurus

“…, n-3 (highly unsaturated fatty acid, HUFA) DHA EPA (eicosapentaenoc acid, 20:5n-3) 0.5-1.5% (Han, 1996;Kikuchi et al, 2011 n-3 (n-3 highly unsaturated fatty acids, n-3 HUFA) (Webster and Lovell, 1990;Ruyter et al, 2000;Lee et al, 2003;Fonseca-Madrigal et al, 2005;Kim and Lee, 2005;Mourente et al, 2005). 40% ( 60%, 40%) , , , 0.5% n-3 HUFA (DHA+EPA) .…”

“…Kim et al, (2010) 16:0 (palmitic acid) 18:1n-9 (oleic acid, elaidic acid) n-3 HUFA , 16:0 18:1n-9 . , , sea bream, channel catfish, Atlantic halibut (Ibeas et al, 1996;Bae et al, 2004;Aksoy et al, 2009;Koizumi et al, 2009;Kikuchi et al, 2011). n-3 HUFA .…”