Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls

Herdmann, Andrea; Nuernberg, Karin; Martin, Jörg; Nuernberg, Gerd; Doran, Olena

doi:10.1017/s1751731110000431

Cited by 44 publications

(62 citation statements)

References 42 publications

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“…The present study demonstrated that the composition of fatty acids in muscles, eggs, and liver can be manipulated by dietary means (Raes et al 2004;Herdmann et al 2010;Oliveira et al 2010;Benatmane et al 2011). In our study, a significant increase in most of the individual liver n-3 PUFA and a decrease in n-6 PUFA were observed in the case of the diet supplemented with FO as the source of 18:3n-3, when compared with CO diet.…”

Section: Discussionmentioning

confidence: 55%

“…Different fatty acid deposition and a lower ratio of n-6/n-3 PUFA in the tissue of bull, rabbit, pig, broiler muscles, and eggs of hens were also achieved by changing the source of dietary fat (Raes et al 2004;Herdmann et al 2010;Oliveira et al 2010;Benatmane et al 2011). In recent years, interest has been focused on the effect of the dietary fat source on the fatty acid deposition in pig, salmon, rabbit liver, etc.…”

Section: Introductionmentioning

confidence: 99%

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Effect of different dietary oil sources on the growth performance, blood characteristics, fatty acid profiles, and expression of lipogenic genes in the liver of broiler chickens

Yan¹,

Zhao²,

Wang³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:The effect of different levels of corn oil (CO) and flaxseed oil (FO) on growth performance, blood characteristics, fatty acid composition, and expression of lipogenic genes in the liver of broiler chickens was studied. Two hundred forty female Cobb-500 broiler chickens at the age of one day (body weight (BW) = 46 ± 4 g) were fed a corn soybean meal based diet containing 5% CO (LC), 3.75% CO + 1.25% FO (FO1), 2.5% CO + 2.5% FO (FO2) or 5% FO (FC). Chickens fed FO1 diet had better BW gain (P = 0.049) and gain/feed ratio (P = 0.006) than those fed LC and FC diets during days 1-21 of age. However, for the whole experimental period (1-42 days of age), the dietary lipid source had no effect on the growth performance. On day 42 of age, the hepatic percentages of 18:3n-3 (P = 0.001) and 20:5n-3 (P < 0.001) were higher in FC than in LC group, which led to a higher content of total n-3 PUFA and lower n-6/n-3 PUFA ratio. The contents of 18:2n-6 (P < 0.05) and Σ n-6 PUFA (P = 0.009) were lower in FC than in LC group. Chickens fed FO1 and FO2 diets had higher Ca 2+ -ATPase activity and lower lipoprotein lipase activity than those fed LC and FC diets, whereas activities of lactate dehydrogenase and Na + ,K + -ATPase were increased by FO2 than by LC diet (P < 0.05). The relative mRNA expression level of lipin 1 in chickens fed FO2 and FC was higher (P < 0.01) than in those fed LC and FO1 diets. Our results demonstrated that higher levels of FO led to hepatic enrichment of n-3 PUFA content and lower n-6/n-3 PUFA ratios in liver and increased the expression of lipin 1 whereas the expression of lipin 2, NADH dehydrogenase subunit 2, Δ-6 fatty acid desaturase, WD and tetratricopeptide repeats 1, and glyceraldehyde-3-phosphate dehydrogenase was not affected.

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Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Effect of different dietary oil sources on the growth performance, blood characteristics, fatty acid profiles, and expression of lipogenic genes in the liver of broiler chickens

Yan¹,

Zhao²,

Wang³

et al. 2015

CZECH J ANIM SCI

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“…All samples were stored at 2808C until fatty acids and protein expression analyses. Previous studies have demonstrated that these storage conditions do not affect D5d and D6d protein expression (Missotten et al, 2009;Herdmann et al, 2010).…”

Section: Methodsmentioning

confidence: 92%

“…The Ca 21 method is widely used for increasing yield of microsomes, especially in cases where the amount of tissue from microsomal preparation is limited (Missotten et al, 2009;Herdmann et al, 2010;Ward et al, 2010). In brief, 5 g of muscle was homogenized in 20 ml Tris-sucrose buffer (10 mM Tris-HCl, 250 mM sucrose, pH 7.4), and the homogenate was centrifuged at 12 0003g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…In porcine m. longissimus dorsi, fish oil, but not linseed, increased the D5d and D6d expression, suggesting that n-3 LC-PUFA could upregulate protein expression (Missotten et al, 2009). Recent beef studies have investigated the role of D5d and D6d enzymes in lipogenesis (Ward et al, 2010) and dietary regulation of expression in backfat and longissimus muscle (Herdmann et al, 2010). The concept of fatty acids as nutraceuticals is important for promoting the development of functional foods as a means of proactive disease prevention.…”

Section: Introductionmentioning

confidence: 99%

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Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and Δ5-, Δ6-desaturase protein expression in steer muscles

Turner

Mitchell

Duynisveld

et al. 2012

Animal

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This study investigated effects of roasted or extruded oilseed supplementation ranging in n-6/n-3 ratios from 0.3 to 5.0 on the fatty acid composition and expression of delta-5 desaturase (D5d) and D6-desaturase (D6d) protein in commercial steer cheek (m. masseter) and diaphragm (pars costalis diaphragmatis) muscles. In general, the n-6/n-3 ratio of the diet had a subsequent effect on the muscle n-6/n-3 ratio (P , 0.05), with muscle 18:2n-6 and 18:3n-3 content relating to proportion of dietary soya bean and linseed (P , 0.01). Compared with canola, pure linseed and soya bean diets reduced 14:1c-9 and 16:1c-9 (P , 0.05) but increased 18:1t-11 and c-9,t-11 conjugated linoleic acid (CLA) content (P , 0.01). Oilseed processing had a minor influence but extruded oilseeds increase 18:1t-11 and c-9,t-11 CLA compared with roasted (P , 0.05). Polar lipid 18:3n-3 and n-3 long-chain polyunsaturated fatty acid (LC, >20 carbons PUFA) derivative content increased in relation to dietary linseed supplementation in the diaphragm (P , 0.01), whereas only 18:3n-3 was increased in the cheek (P , 0.01). Protein expression did not differ between diets; however, in each muscle the D5d protein expression had a stronger association with the desaturase products rather than the precursors. The relationship between D5d protein expression and the muscle LC n-6/n-3 ratio was negative in both muscles (P , 0.05). The relationship between D6d protein expression and the LC n-6/n-3 ratio was positive in the cheek (P , 0.001) and negative in the diaphragm (P , 0.05). In conclusion, diet n-6/n-3 ratio affected muscle 18:2n-6 and 18:3n-3 deposition, whereas the D5d and D6d protein expression had some influence on the polar lipid LC-PUFA profile. Results reaffirm that processed oilseeds can be used to increase the proportion of fatty acids potentially beneficial for human health, by influencing the formation of LC-PUFA and reducing the n-6/n-3 ratio.

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Expression of lipid metabolism related genes in subcutaneous adipose tissue from Chilota lambs grazing on two different pasture types

Gallardo

Cárcamo

Hiller

et al. 2014

Euro J Lipid Sci & Tech

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The focus of the present study was to investigate how the different chemical and botanical composition of two different pasture types, Calafatal pasture (CP) and naturalized pasture (NP), affect gene/protein expression levels of lipogenic enzymes and transcription factors (ACC, FAS, SCD1, and SREBP‐1c) as well as the concentrations of their fatty acid products in subcutaneous adipose tissue of Chilota lambs. Sixteen male Chilota lambs kept under different grazing conditions, CP versus NP, in Chiloé Archipelago (Chile) were included in the experiment. QRT‐PCR, western blot and fatty acid analysis were performed of subcutaneous adipose tissue samples. Compared to NP, CP diet induced a 1.51‐fold increase of ACACA expression, a 1.60‐fold rise of FASN expression and a 1.56‐fold elevation of SREBF1 expression. SCD gene expression did not significantly differ between both feeding groups. Different pasture types did not affect protein expression levels of lipogenic enzymes (ACC, FAS, and SCD1) and transcription factor pSREBP‐1c (precursor form) and mSREBP‐1c (mature form) in subcutaneous fat of lambs. Amounts of single and the sum of saturated fatty acids were significantly higher in subcutaneous fat samples of lambs fed NP than of lambs fed CP. Concentrations of single PUFA and the sum n‐3 and n‐6 PUFA and the n‐6/n‐3 PUFA ratio were not affected by different pasture types. The inclusion of different adipose tissue depots and more sampling “endpoints” during animal growth may be beneficial to comprehensively elucidate the mechanisms by which dietary PUFA intervention affects the lipid metabolism of adipose tissues in lambs. Practical applications: Increasing public awareness of the health benefits attributable to PUFA (n‐3 PUFA) has stimulated interest in sources of these fatty acids for human consumption. In relation to lamb adipose and muscle tissue, it is considered a good source of nutrients such as PUFA, especially if lambs are fed on pastures rather than concentrates. This study provides deeper insights in the clarification of the regulation of fatty acid biosynthesis and fat partitioning in tissues of ruminants with special emphasis on lambs. Relative gene expression differences between subcutaneous fat samples of Chilota lambs grazing on two different pasture types, Calafatal pasture (CP) and naturalized pasture (NP).

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Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls

Cited by 44 publications

References 42 publications

Effect of different dietary oil sources on the growth performance, blood characteristics, fatty acid profiles, and expression of lipogenic genes in the liver of broiler chickens

Effect of different dietary oil sources on the growth performance, blood characteristics, fatty acid profiles, and expression of lipogenic genes in the liver of broiler chickens

Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and Δ5-, Δ6-desaturase protein expression in steer muscles

Expression of lipid metabolism related genes in subcutaneous adipose tissue from Chilota lambs grazing on two different pasture types

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