Effect of dietarytrans fatty acids on some membrane‐associated enzymes and receptors in rat heart

Abstract

Three groups of male weanling Sprague-Dawley rats were fed diets containing 20% corn oil, 20% partially hydrogenated soybean oil (PHSBO) or 18% PHSBO + 2% corn oil. PHSBO contained about 48% of its total fatty acids as trans-octadecenoate. Rats were killed after 16-18 weeks of feeding the various diets, hearts were dissected and crude sarcolemma was prepared by differential centrifugation. The activities of ouabain-sensitive (Na+ + K+)ATPase were significantly lower in membranes of rats fed 20% PHSBO than the control rats fed 20% corn oil. The feeding of 2% corn oil with 18% PHSBO resulted in partial restoration of the enzyme activity. The maximum number of [3H]ouabain-binding sites (Bmax) was also lower in cardiac membranes of rats fed 20% PHSBO than those fed 20% corn oil. Similar to (Na+ + K+)-ATPase activity, some restoration of the number of [3H]ouabain-binding sites was observed when 2% corn oil was fed with 18% PHSBO-containing diet. There was no difference in the binding affinity of the radioligand for the receptor among the 3 dietary groups. Adenylate cyclase activities (fluoride-, isoproterenol- and forskolin-stimulated) were lower in membranes of rats fed 20% PHSBO or 18% PHSBO + 2% corn oil than in the control group fed 20% corn oil. Density of the beta-adrenergic receptor was the lowest in cardiac membranes of rats fed 20% PHSBO. The feeding of 2% corn oil with 18% PHSBO resulted in partial restoration of the maximum number of [3H]dihydroalprenolol (DHA)-binding sites.(ABSTRACT TRUNCATED AT 250 WORDS)

Toxicological aspects of trans fat consumption over two sequential generations of rats: Oxidative damage and preference for amphetamine

“…also conferred greater rigidity to neuronal membranes (Alam et al, 1989). Since SO is rich in n-6 FA, this supplementation promoted an increased incorporation of n-6 PUFA derivatives in the hippocampus, as also observed in first generation rats supplemented with SO .…”

Ácidos Grasos Con Isomería Trans I: Su Origen Y Los Efectos en La Salud Humana

“…Los AGT, tiene un punto de fusión mayor que su contraparte cis y una orientación espacial similar a los ácidos grasos saturados, por lo cual reducen la fluidez de la membrana, modulando de manera diferente la actividad de las proteínas insertas en ella, produciendo alteraciones en la estructura celular y en sus funciones. Los AGT, aumentan la fragilidad osmótica de los eritrocitos (43), producen hinchamiento y reducción del consumo de oxígeno y de la producción de ATp en las mitocondrias (44), favorecen el desarrollo de arritmias experimentales en miocitos (45), inhiben la bomba Na/K ATPasa (46), y modifican la actividad de la enzima adenilato ciclasa, que forma AMP cíclico (47).…”

Comparison of Phosphatidylcholine Vesicle Properties Related to Geometrical Isomerism†

“…The effect of trans fatty acid intake has been the subject of several investigations, which evidenced how trans isomers can be metabolized and become constituents of membrane phospholipids. Epidemiological studies have also linked this intake with health complications (3–7). In an effort to understand the potential harmful effects of trans phospholipids in membranes, some recent articles reinvestigated the different membrane properties induced by cis and trans geometries (8, 9).…”