Effect of Dietary Lipids and Vitamin E on in Vitro Lipid Peroxidation in Rat Liver and Kidney Homogenates

Abstract

Rats were fed for 5 wk 10% (wt/wt) menhaden oil (MO) or a 10% corn oil-lard (COL) mixture (1:1) in diets with a low vitamin E content (less than or equal to 5 mg/kg) or supplemented with d-alpha-tocopheryl succinate to a total of 30 or 150 mg per kg. Thiobarbituric acid-reactive substances (TBARS), conjugated dienes (CD), hexanal and total volatiles (TOV) were measured in tissue homogenates incubated at 37 degrees C for 1 h in the absence (uninduced) and presence of 15 microM ferrous sulfate (induced). The fatty acid composition of liver and kidney reflected that of dietary lipids. For uninduced peroxidation, there was in general a significant inverse correlation of TBARS, CD and TOV with the log of dietary vitamin E content for liver and kidney from rats fed either lipid. For induced peroxidation, the inverse correlation was significant for liver, but not for kidney, from rats fed either lipid. The correlation was generally higher for liver and kidney from rats fed COL than for tissues from rats fed MO. Vitamin E was thus a more effective antioxidant for liver than for kidney regardless of the dietary lipid, and for liver and kidney from rats fed COL than from rats fed MO. Dietary MO enhanced tissue susceptibility to both peroxidation systems. A simulation model developed to mimic the experiments showed good correlations between experimental data and simulated values.

The Influence of Dietary Fatty Acids and Vitamin E on Plasma Prostanoids and Liver Microsomal Alkane Production in Broiler Chickens with Regard to Nutritional Encephalomalacia.

“…However, the pentane production originating from n-6 fatty acids was considerably higher in the deficient group fed linoleic acid. Comparable results have been obtained earlier in vitamin E-deficient rats fed corn oil or menhaden oil (19). TBA-reactive substances arising mainly from n-3 fatty acids were 1.38 times higher, when the rats received menhaden oil .…”

Antioxidative and Hypolipidemic Effects of Dietary Green Tea Polyphenols in ddY Mice Fed Fish Oil-rich Diet

“…Administration of fish oil to mice increased the contents of n-3 PUFA in the liver. Hu et al 47) and Leibovitz et al48) also reported similar extents of increase of PUFA contents in the liver of rats fed a fish oil-supplemented diet. Further increases of the contents of n-3 PUFA were observed in the liver of the FO+TP group in this study.…”

Effects of NaCl and Lime water on the Hypoglycemic and Antioxidant activities of Ocimum gratissimum in Alloxan-induced Diabetic rats.

“…Under normal physiological conditions, the concentrations of lipid peroxides in the tissues are low. Hu et al, [29] reported elevated levels of lipid peroxides in the plasma of diabe tic rats. Lipid peroxide-mediated tissue damage results in the development of both type I and type II diabetes.…”