Effect of dietary manganese on antioxidant status and expressions of heat shock proteins and factors in tissues of laying broiler breeders under normal and high environmental temperatures

Abstract

To investigate the effect of Mn on antioxidant status and on the expressions of heat shock proteins/factors in tissues of laying broiler breeders subjected to heat challenge, we used a completely randomised design (n 6) with a factorial arrangement of 2 environmental temperatures (normal, 21±1°C, and high, 32±1°C)×3 dietary Mn treatments (a Mn-unsupplemented basal diet (CON), or a basal diet supplemented with 120 mg Mn/kg diet, either as inorganic Mn sulphate (iMn) or as organic Mn proteinate (oMn)). There were no interactions (P>0·10) between environmental temperature and dietary Mn in any of the measured indices. High temperature decreased (P<0·003) Mn content, and also tended (P=0·07) to decrease Cu Zn superoxide dismutase (CuZnSOD) activity in the liver and heart. However, an increased Mn superoxide dismutase (MnSOD) activity (P<0·05) and a slight increase in malondialdehyde level (P=0·06) were detected in breast muscle. Up-regulated (P<0·05) expressions of heat shock factor 1 (HSF1) and HSF3 mRNA and heat shock protein 70 (HSP70) mRNA and protein were found in all three tissues. Broiler breeders fed either iMn or oMn had higher tissue Mn content (P<0·0001), heart MnSOD and CuZnSOD activities (P<0·01) and breast muscle MnSOD protein levels (P<0·05), and lower (P<0·05) breast muscle HSP70 mRNA and protein levels compared with those fed CON. Broiler breeders fed oMn had higher (P<0·03) bone Mn content than those fed iMn. These results indicate that high temperature decreases Mn retention and increases HSP70, HSF1 and HSF3 expressions in the tissues of laying broiler breeders. Furthermore, dietary supplementation with Mn in either source may enhance the heart's antioxidant ability and inhibit the expression of HSP70 in breast muscle. Finally, the organic Mn appears to be more available than inorganic Mn for bone in laying broiler breeders regardless of environmental temperatures.

Astragalus polysaccharide supplementation improves production performance, egg quality, serum biochemical index and gut microbiota in Chongren hens

“…Oxidative stress mainly occurs during the fastigial of laying hens. It is caused by the imbalance between the output of reactive oxygen species and the antioxidant system (Zhu et al., 2016). As the age of the laying hen increases, the activity of antioxidant enzymes in the body decreases, and the accumulation of free radicals causes lipid peroxidation and damages cell function (Sørensen et al., 2010).…”

Manganese and Its Role in Poultry Nutrition: An Overview

“…The Mn metalloenzymes include arginase, phosphoenolpyruvate decarboxylase, glutamine synthetase, as well Mn-superoxide dismutase (Mn-SOD). In particular, MnSOD is significantly expressed in organs including an elevated mitochondria number such as heart, liver and kidneys (Li et al, 2011;Bai et al, 2012;Zhu et al, 2016). Among tissues expressing MnSOD in human (Marklund, 1980), mice (Jones et al, 1995) and chicken (Kong et al, 2003;Zhu et al, 2016), heart has the highest steady state mRNA expression level.…”

“…In particular, MnSOD is significantly expressed in organs including an elevated mitochondria number such as heart, liver and kidneys (Li et al, 2011;Bai et al, 2012;Zhu et al, 2016). Among tissues expressing MnSOD in human (Marklund, 1980), mice (Jones et al, 1995) and chicken (Kong et al, 2003;Zhu et al, 2016), heart has the highest steady state mRNA expression level. Previous trials using chickens stated that, even if Mn level is significantly lower in heart when compared to other tissues, the activity of MnSOD is higher and very sensitive to supplemental Mn levels in corn-soybean diets (Luo et al, 1991;Luo et al, 1992).…”

Sex‐dependent metal accumulation and immunoexpression of Hsp70 and Nrf2 in rats' brain following manganese exposure

“…18 Particularly, Mn overexposure has been reported to increase Hsp70 levels in non-nervous as well as brain tissues in experimental models. 5,[19][20][21] Similarly, Nrf2, a key regulator of the endogenous antioxidant response to oxidative stress can be modulated by metal exposures. Nrf2 triggers the transcription of endogenous antioxidant enzymes such as glutathione, catalase, superoxide dismutase amongst others, through its interaction with the antioxidant responsive element (ARE).…”