Effect of Dietary Oligosaccharides on the Performance, Intestinal Microflora and Serum Immunoglobulin Contents in Laying Hens

Abstract

This study was conducted to investigate the effects of dietary supplementation of mannan-oligosaccharides (MOS) and fructo-oligosaccharides (FOS) on the performance, immune response and small intestinal microflora in laying hens. A total of 960 Hy-Line Brown Ⓡ laying hens of 27 wks old, housed in 2 bird cages, were assigned in a completely randomized block design into one of the following 6 dietary treatments: control, antibiotic (6 ppm avilamycine), 0.025% MOS, 0.05% MOS, 0.25% FOS, and 0.5% FOS. Each treatment had 4 replicates of 40 birds and was fed ad libitum for 6 wks under 16 h lighting regimen. There were significant differences among treatments in hen-day and hen-housed egg production. Hen-day egg production in 0.025% MOS was significantly higher than that of control. Hen-housed egg production in antibiotic-treated group was significantly higher compared with control. Egg weight, feed intake and feed conversion were not significantly different among treatments. Egg shell thickness was highest in 0.25% FOS, but was not significantly different among the rest of treatments. There were no significant differences among treatments in egg shell strength, egg shell color, egg yolk color and Haugh unit. IgG concentrations in serum were not significantly different among treatments. On the other hand, IgA concentrations of the treated birds tended to be increased compared with control. Dietary treatments tended to decrease Cl. perfringens and E. coli, and to increase Lactobacillus spp. The result of this experiment showed that dietary supplementation of MOS and FOS in laying hens tended to improve egg production comparable to the supplementation of antibiotics. The level of serum IgA and small intestinal microflora were also significantly affected by the treatments.

Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers

“…In other studies, however, IgA was increased in laying hens (Kim et al, 2009) ed that IgG levels were increased by MOS supplementation in turkeys, and Woo et al (2007) reported that IgG was increased by MOS supplementation in layers. Yin et al (2008) reported that supplementation with a prebiotic oligosaccharide galacto-mannan increased the serum levels of IgA, IgG, and IgM compared with the level of lincomycin in early-weaned pigs.…”

“…Mikkelsen et al (2004) reported that a high level of FOS increases gas production by intestinal microbiota, which causes diarrhea. Dietary mannan oligosaccharides improved broiler and turkey performance at a level of 0.0125 to 0.1% (Hooge, 2003;Sims et al, 2004) and improved the egg production of layers at a level of 0.025 to 0.05% (Woo et al, 2007;Kim et al, 2009).…”

“…Therefore, mannose can result in the movement of undesirable bacteria through the intestine without colonization (Newman, 1994). Supplemental MOS has also been shown to increase the production of IgA in laying hens (Kim et al, 2009), rats (Kudoh et al, 1999), and dogs (Swanson et al, 2002a,b). Immunoglobulin A inhibits the attachment and penetration of bacteria in the lumen, increases the production of mucus (McKay and Perdue, 1993), and prevents inflammation that could cause epithelial tissue damage (Russell et al, 1989).…”

Effect of adjuvants on in ovo vaccination against Newcastle disease on hatchability, performance and antibody titres in commercial pullets

“…The ability of MOS to mature dendritic cells was shown to be mediated through a TLR4‐dependent mechanism (Sheng et al., ). It is also reported that supplementation of MOS increased the synthesis of Ig, particularly IgA (Kim et al., ). In this context, Avinew ND vaccine combined with AH and/or MOS adjuvants was used to vaccinate embryos of commercial pullets in our study to examine the efficacy of these types of vaccinations.…”

Effects of mannan-oligosaccharides-supplemented diets on production performance of four close-bred flocks of Japanese quail breeders

“…The decrease in pathogens and increase in beneficial bacteria may be because of competition for receptor sites, and the production of volatile fatty acids, of some bacteriocins, and of IgA antibodies by the immune system of the host (Brzoska et al, 2007;Kim et al, 2009). Owing to these beneficial changes in microflora, the number of goblet cells and length of intestinal villi increase, which ultimately promotes the health and functions of the GIT of the host (Rehman et al, 2009;Bonos et al, 2010;Vahdatpour et al, 2011).…”