This study was conducted to investigate the effects of dietary probiotics and immunomodulator on growth performance, carcass characteristics, fecal NH3 content and pathogenic bacteria counts in ileum and cecum and ileum of broiler chicken (Korean native chicken, HanHyup No. 3). A total of 120 (day-old) chicks were randomly divided into 5 treatments with 3 replicates and there were 8 birds per replicate. Dietary treatments consisted of five diets; the corn-soybean based control diet (C), the diet containing antibiotics (Avilamycin) 10 ppm (T1), the diet containing probiotics 1 [(Lactobacillus (4.45×10 6 ) + yeast (1.51×10 6 ) + Bacillus subtilis (3.50×10 5 )] at 0.5% level (T2), probiotics 2 [(Lactobacillus (6.70×10 7 ) + yeast (3.10×10 6 )] at 0.5% level in diet (T3), and the diet containing probiotics 3 [T2 + β-glucan + organic acid] (T4) and raised for 9 weeks. There were no significant differences among treatments in weight gain, feed intake and feed conversion. Carcass ratios of broilers were higher in T3 and T4 than other treatments, however, the differences were non-significant. Internal organs and liver, heart weight were significantly increased in T4 (p<0.05) compared to other treatments. The fecal NH3 gas content was decreased (p<0.05) in antibiotics fed group than others. However, probiotic fed groups were not different when compared with control. The number of Salmonella and E. coli in cecum were reduced in the group supplemented with probiotics and immunomodulator compared to the antibiotics (p<0.05). In this experiment, we showed that diets containing pro-biotics and immunomodulator were capable of an alternative to antibiotics.