The present study was carried out to evaluate the influence of dietary supplementation of probiotic bacteria (Lactobacillus acidophilus), on growth performance of Labeo rohita. The probiotic L. acidophilus was procured from the market and the feed was prepared by crushing the basal food with probiotic bacteria in different combinations. The feeding trials were conducted in triplicate for 60 days, to determine the effect of dietary probiotics on growth status of fish. For this, the live fishes (L. rohita) were captured from Sagar Taal, Budaun, U.P. After acclimatization for 10 days, the fishes with similar body weight were distributed randomly into five treatment groups at