Effect of Dietary Protein Level on Lipid Metabolism in Growing Chicks

Abstract

The influence of dietary protein on hepatic lipogenesis in growing chicks was investigated. In vitro study using liver slices demonstrated that the incorporation of acetate-1-14C or glucose-U-14C into fatty acids and glycerids-glycerol was significantly depressed as the protein level increased. Activity of NADP-isocitrate dehydrogenase was lower (P<0.01) in chicks fed 10% protein diet. Activities of the pentose pathway dehydrogenases significantly decreased as the dietary protein level increase from 10% to 40%. Increasing the dietary protein level from 10% to 40% significantly decreased the activity of NAD-malic dehydrogenase. The possible regulatory mechanisms responsible for the depression of hepatic lipogenesis in chicks fed a high protein diet are discussed. It is suggested that a limitation in the availability of cytoplasmic reducing equivalents may initiate the reduction in hepatic fatty acid synthesis of chicks fed a high protein diet. In addition, concentration of various lipids in liver and serum was affected by dietary protein level. Jap.

Effect of driedBacillus subtilisculture on growth, body composition and hepatic lipogenic enzyme activity in female broiler chicks

“…The various lipid fractions were separated by thin-layer chromatography on silica-gel chromarods using hexanAiethy1 ether-formic acid (60 : 10: 0.1, by vol.) and hexanebenzene (1 : 1, v/v) as developing solvents, and quantified using an Iatroscan TH-10 TLC/FID Analyser (Iatron Laboratories Inc., Tokyo, Japan) (Tanaka et al 1979). Carcass composition was determined by the method of the Association of Official Analytical Chemists (1980).…”

Effect of dietary essential fatty acid deficiency on hepatic lipogenesis in the growing chick.

“…The experimental procedures used to determine enzyme activities in the liver as well as the liver contents and the serum concentrations of various lipid fractions have been described previously14, 16,17). All the data were statistically analyzed using the "t-test".…”