Effect of dietary resveratrol supplementation on meat quality, muscle antioxidative capacity and mitochondrial biogenesis of broilers

Zhang, Cheng; Yang, Lei; Xian-qing, Zhao; Chen, Xingyong; Wang, Li; Zhao, Geng

doi:10.1002/jsfa.8576

Cited by 51 publications

(42 citation statements)

References 32 publications

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“…The reaction was performed in a final volume of 20 μL, consisting of 10 μL 2 × SYBR Green Fast qPCR Mix with Low Rox, 0.4 μL reverse primers (10 mmol L −1 ), 0.4 μL forward primers (10 mmol L −1 ), 8.8 μL doubled‐distilled water and 0.4 μL cDNA template. The thermal cycler parameters were as follows: 95 °C for 2 min, 40 cycles of 95 °C for 15 s, and 60 °C for 30 s. The melting curve conditions were as follows: 1 cycle at 95 °C for 5 s, 60 °C for 60 s, and 95 °C for 15 s. The mRNA expression levels of target genes were calculated using the 2 −ΔΔCt method and normalized to the mRNA level of β‐actin …”

Section: Methodsmentioning

confidence: 99%

Effect of l‐theanine on growth performance, intestinal development and health, and peptide and amino acid transporters expression of broilers

et al. 2020

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BACKGROUND: L-Theanine has multiple beneficial biological activities. However, there is little information about the use of L-theanine in broiler production. Therefore, this study investigated the effect of L-theanine on growth performance, intestinal development and health, and the mRNA levels of intestinal peptide and amino acid (AA) transporters of broilers. RESULTS: Body weight and average daily gain were increased by L-theanine, whereas feed to gain ratio was decreased (quadratic, P < 0.05). Notably, the relative weight of duodenum, jejunum and ileum, villus height, villus height to crypt depth ratio, the jejunal activities of glutathione peroxidase, total antioxidant capacity, catalase and total superoxide dismutase were increased linearly and/or quadratically by L-theanine (P < 0.05), whereas crypt depth, serum D-lactic acid, and jejunal protein carbonyls and malondialdehyde content were decreased linearly and/or quadratically (P < 0.05). Moreover, L-theanine enhanced the jejunal mRNA levels of occludin, claudin-1, E-cadherin, zona occludens-1, di-and tripeptide transporter, excitatory AA transporter 3, Na + -independent cationic AA transporter 1, Na + -independent cationic and zwitterionic AA transporter, Na + -and Cl − -dependent neutral and cationic AA transporter, Na + -independent cationic and Na + -dependent neutral AA transporter (y+LAT) 1, y+LAT2, Na + -independent branched-chain and aromatic AA transporter, and heavy chain corresponding to the b ∘,+ transport system (linear and/or quadratic, P < 0.05). CONCLUSIONS: L-Theanine beneficially affected the growth performance of broilers by improving intestinal development andhealth, and the intestinal mRNA levels of AA and peptide transporters. Therefore, L-theanine has the potential to be a promising feed additive for broilers. Jejunum morphology analysisJejunum morphology was measured as previously described. 21,22 Briefly, the formalin-fixed jejunal samples were embedded in paraffin wax, cut into about 5 μm transverse sections, mounted on glass slides, and then stained with hematoxylin and eosin. Crypt depth and villus height were determined with an image processing and analysis system (Leica Imaging Systems Ltd, Cambridge, UK). Fifteen well-orientated villi and their adjacent crypts from each intestinal sample were measured. Serum D-lactic acid content and jejunum antioxidant status analysisThe mucosa of jejunum was homogenized in ice-cold physiological saline solution (1:9, w/v). The homogenate was centrifuged at 2500 × g for 8 min at 4 ∘ C and then the supernatant was collected for antioxidant enzyme activity and metabolite content analysis at appropriate dilution. The protein concentration of the supernatant was assayed by the Bradford method with bovine serum albumin as the standard. The content of serum D-lactic acid and RESULTS Growth performanceGrowth performance data are shown in Table 3. L-Theanine elevated BW at days 21 and 42 (quadratic, P < 0.05). From days 1 to 21, L-theanine increased ADG (quadratic, P < 0.05) and decreased

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Section: Methodsmentioning

confidence: 99%

Effect of l‐theanine on growth performance, intestinal development and health, and peptide and amino acid transporters expression of broilers

et al. 2020

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“…The PCR mixture consisted of 10 μL of SYBR Premix Ex Taq ll (Tli RNaseH Plus), 1 μL of each primer, 2 μL of cDNA sample, and 6 μL ddH 2 O. The PCR cycling parameters were in accordance with our previous study . The target gene mRNA expression level was calculated according to the 2 − ΔΔ Ct method, and the β‐actin gene was used as the internal standard.…”

Section: Methodsmentioning

confidence: 99%

“…The PCR cycling parameters were in accordance with our previous study. 26 The target gene mRNA expression level was calculated according to the 2 −ΔΔCt method, 27 and the -actin gene was used as the internal standard.…”

Section: Real-time Pcr Analysismentioning

confidence: 99%

Protective effects of resveratrol against high ambient temperature‐induced spleen dysplasia in broilers through modulating splenic redox status and apoptosis

et al. 2018

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“…RSV has been studied in both animals and humans, and is associated with a wide range of biological effects and cellular pathways. Amongst its properties, it has been shown that RSV acts as an anti-inflammatory [ 125 ], anti-oxidant [ 126 ], anti-diabetic [ 127 ], as well as an osteoprotective agent [ 128 , 129 ] and exercise mimetic [ 129 , 130 ], making it an exceptional candidate to help mitigate muscle deconditioning in our partial gravity analog model. We find that a moderate does of RSV appears to blunt reductions in front and back paw grip force as well as partially protect myofiber cross-sectional area in both the soleus and the gastrocnemius [ 41 ].…”

Section: Future Perspectivesmentioning

confidence: 99%

Approaching Gravity as a Continuum Using the Rat Partial Weight-Bearing Model

Mortreux

Rosa‐Caldwell

2020

Life

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For decades, scientists have relied on animals to understand the risks and consequences of space travel. Animals remain key to study the physiological alterations during spaceflight and provide crucial information about microgravity-induced changes. While spaceflights may appear common, they remain costly and, coupled with limited cargo areas, do not allow for large sample sizes onboard. In 1979, a model of hindlimb unloading (HU) was successfully created to mimic microgravity and has been used extensively since its creation. Four decades later, the first model of mouse partial weight-bearing (PWB) was developed, aiming at mimicking partial gravity environments. Return to the Lunar surface for astronauts is now imminent and prompted the need for an animal model closer to human physiology; hence in 2018, our laboratory created a new model of PWB for adult rats. In this review, we will focus on the rat model of PWB, from its conception to the current state of knowledge. Additionally, we will address how this new model, used in conjunction with HU, will help implement new paradigms allowing scientists to anticipate the physiological alterations and needs of astronauts. Finally, we will discuss the outstanding questions and future perspectives in space research and propose potential solutions using the rat PWB model.

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Effect of dietary resveratrol supplementation on meat quality, muscle antioxidative capacity and mitochondrial biogenesis of broilers

Abstract: Resveratrol can be used as a feed additive to improve meat quality of broilers, which may be associated with improved muscle antioxidative status and mitochondrial biogenesis. © 2017 Society of Chemical Industry.

Cited by 51 publications

References 32 publications

Effect of l‐theanine on growth performance, intestinal development and health, and peptide and amino acid transporters expression of broilers

Effect of l‐theanine on growth performance, intestinal development and health, and peptide and amino acid transporters expression of broilers

Protective effects of resveratrol against high ambient temperature‐induced spleen dysplasia in broilers through modulating splenic redox status and apoptosis

Approaching Gravity as a Continuum Using the Rat Partial Weight-Bearing Model

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