Effect of dietary safflower oil upon lipogenesis in neonatal lamb

Vernon, Richard G.

doi:10.1007/bf02532701

Cited by 39 publications

(16 citation statements)

References 30 publications

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“…mg protein-', at 3O'C) [6]. In addition only 19% of the PDH in sheep adipose tissue was in the active state compared with 40-50% in adipose tissue from rats fed normal chow [8], but this is similar to the proportion of PDH in the active state in fasted or diabetic rats or rats fed a high fat diet for 6 days (12-18%) [6]. Adipose tissue from rats in these latter stat& has a low rate of fatty acid synthesis from glucose and a relatively high proportion of glucose utilized is released from the tissue as lactate [6,11,12].…”

Section: Adipose Tissuementioning

confidence: 94%

“…Protein concentration of the homogenates was determined by the method in [7]. The rate of incorporation of 14C from [U-14C]glucose into fatty acids in sheep adipose tissue slices was measured as in [8] except that the incubation medium (Krebs-Ringer bicarbonate) contained 3 mM glucose, 0.6 mM acetate, 1 mM L-lactate and 0.1 pg . ml-' insulin.…”

Section: Materials Aud Methodsmentioning

confidence: 99%

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Pyruvate dehydrogenase and the regulation of glucose metabolism in ruminant tissues

1980

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Section: Adipose Tissuementioning

confidence: 94%

Section: Materials Aud Methodsmentioning

confidence: 99%

Pyruvate dehydrogenase and the regulation of glucose metabolism in ruminant tissues

1980

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“…Moore & Christie (1984) suggested that when ruminants are fed large quantities of unsaturated fats (as in the present study) the hydrogenation capacity of the rumen is exceeded. The changes in fatty acid composition of some tissues were described in lambs fed PUFA-supplemented diets (Vernon, 1975;Muci et al, 1992a;Enser et al, 1998). Chang et al (1992) concluded that the unsaturated fatty acid composition of the ruminant tissues may be modified through the diet if the fatty acids escaped ruminal changes.…”

Section: Resultsmentioning

confidence: 99%

Effect of extruded cotton and canola seed on unsaturated fatty acid composition in the plasma, erythrocytes and livers of lambs

Ahmadi¹,

Golian²,

Akbarian³

et al. 2011

SA J. An. Sci.

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An experiment was conducted to study the effects of extruded cotton seed (ECOS) and canola seed (ECAS) in the diet of male Mehraban lambs on the concentration of unsaturated fatty acids in their plasma, erythrocytes and livers. The treatments were: (1) control (C); (2) diet C+6% ECAS, (3) diet C+6% ECOS, (4) diet C+12% ECAS, (5) diet C+12% ECOS, (6) diet C+6% ECAS+6% ECOS, (7) diet C+12% ECAS +6% ECOS, (8) diet C+6% ECAS +12% ECOS, (9) diet C+12% ECAS+12% ECOS, (10) diet C+18% ECAS+18% ECOS. A complete randomized experimental design was applied. Sixty lambs (5 -6 months of age) were randomly allocated to the 10 dietary treatments in order to have six lambs (replicates) per diet. The lambs were housed in individual pens. The average weight of lambs at the onset of the study was 34.3 ± 2.12 kg. The experiment lasted for 90 days. Results indicated that the concentration of oleic acid (C18:1), linoleic acid (C18:2 n-6) and linolenic acid (C18:3 n-3) in the plasma and oleic acid and linolenic acid in erythrocyte lipids were higher in lambs fed diets containing oil seeds or a combination of the oil seeds compared to those receiving the control diet. The supplementation of 6% and 12% ECAS increased the linoleic acid concentration in the liver lipids significantly compared to those fed the diets containing ECOS. There was a linear correlation between linoleic acid content of the liver and levels of dietary ECAS and ECOS. This study provides evidence that dietary oleic acid, linoleic acid and linolenic acid might escape biohydrogenation in the rumen and showed that the type of dietary fat has a marked impact on lipid metabolism in the liver. ________________________________________________________________________________

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“…However. in neonatal animals celiular hypertrophy and &a;ked variations'in adipocyte sizes in animals of the same chronological age can complicate the evaluation of age-related changes in adipose tissue metabolism (3,15). Fat cells from fetal calves can be expected to be smaller than those of newborn calves (3).…”

Section: Glycerol For Fatty Acid Esterification Pentose Phosphatementioning

confidence: 99%

Glucose, Acetate, and Lactate Metabolism in Perirenal Adipose Tissue of Fetal and Newborn Calves

Wijayasinghe

1986

Pediatr Res

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ABSTRACT. Rates of utilization of glucose, acetate, and cerning such changes in lipid metabolism have yet to be obtained. lactate and activities of selected enzymes were determined The present investigation involved studies of adipose tissue from in vitro to characterize the nature of lipogenesis and me-fetal and unsuckled newborn calves. Rates of utilization of tabolite utilization in perirenal adipose tissue from 6-to 7-glucose, acetate, and lactate, as substrates for oxidation and for month old fetal and 3-to 4-h-old unsuckled newborn calves. synthesis of fatty acids and glyceride glycerol, were determined Contribution of the pentose phosphate cycle to glucose in vitro to characterize the nature of lipogenesis and patterns of metabolism was estimated using specifically labeled glu-metabolite utilization. Specifically labeled glucose was employed cose. Rates of fatty acid synthesis from all three substrates to evaluate the contribution of the pentose phosphate cycle to and oxidation of glucose were much greater in fetal than glucose metabolism and to the reducing equivalents needed for in newborn adipose tissue. In fetal adipose tissue, acetate fatty acid synthesis. In addition, activities of selected enzymes and lactate were major sources of carbon for fatty acid related to lipogenesis in adipose tissue were also assayed. synthesis; glucose functioned mainly by metabolism via the pentose phosphate cycle to provide reducing equivalents for fatty acid synthesis and by incorporation into glyceride MATERIALS AND METHODS glycerol for fatty acid esterification. Pentose phosphatePerirenal adipose tissue samples were obtained from five fetal cycle contributed 58 and 12% to glucose metabolism in calves (180-2 10 days gestation or 64-75% of full term) and eight adipose tissue of fetal and newborn calves, respectively. newborn unsuckled calves (3-4 h after birth). Newborn calves Adipose tissue metabolism of newborn calves was charac-were sacrificed by exsanguination prior to tissue removal. ~d iterized by greatly depressed rates of fatty acid synthesis pose tissue samples were placed in warn saline (37" C) for despite high enzyme activities and elevated rates of gl~cer-transport to the laboratory, cleaned of connective tissue and ide glycerol synthesis. (Pediatr Res 20: 542-544, 1986) blood vessels, and cut into small (30-50 mg) pieces. Adipose tissue pieces weighing 50-100 mg were placed in siliconized 25-ml Erlenmeyer flasks containing 2 ml of incubation medium (Table 1). Concentrations of glucose, acetate, Iactate/pyruvate, M~~~ studies of fetal metabolism have been carried out in and insulin in the incubation medium were determined in the sheep with substrate utilization usually being determined on the preliminary experiment to achieve maximum rates of substrate basis of rates of oxygen consumption and uptake of different utilization. The flasks were gassed with a mixture of 95% O2 and substrates. Various acute and chronic preparations and tech-5% C02, capped with self-sealing rubber serum caps fitted wth niques suc...

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Effect of dietary safflower oil upon lipogenesis in neonatal lamb

Cited by 39 publications

References 30 publications

Pyruvate dehydrogenase and the regulation of glucose metabolism in ruminant tissues

Pyruvate dehydrogenase and the regulation of glucose metabolism in ruminant tissues

Effect of extruded cotton and canola seed on unsaturated fatty acid composition in the plasma, erythrocytes and livers of lambs

Glucose, Acetate, and Lactate Metabolism in Perirenal Adipose Tissue of Fetal and Newborn Calves

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