Effect of dietary selenium and vitamin E on the biomechanical properties of rabbit bones

Abstract

It is generally agreed that combined deficiency of selenium and vitamin E leads to several abnormalities including Kashin-Beck disease which is an endemic and chronic degenerative osteoarthrosis. The abnormalities can be reversed by the administration of various forms of selenium and vitamin E. The present study was designed to investigate the effects of dietary selenium and vitamin E on bone tissue and on the biomechanical properties of bone. Young rabbits of both sexes were fed with either a selenium- and vitamin E-adequate diet (control group), or a selenium- and vitamin E-deficient diet or a selenium-excess diet. The selenium-deficient diet resulted in a significant decrease in plasma selenium level and the selenium-excess diet resulted in a significant increase in the plasma selenium level with respect to the corresponding control values (p < 0.05). The diets did not affect the blood cell counts considerably but erythrocyte glutathione peroxidase activity increased (decreased) relatively when the plasma selenium level increased (decreased) (p < 0.05). The light microscopic investigations of the bone tissues of the two experimental groups indicate that the findings of the present work are compatible with osteomalacia. The biomechanical properties of the bones from the three groups were determined experimentally with bending tests. Both the Se- and vitamin E-deficient diet and the Se-excess diet decreased the biomechanical strength of the bones significantly while the bones belonging to the control group always had the largest modulus of elasticity (p < 0.05).

Acute and subchronic co-administrations to cadmium, diazinon and selenium induce apparent osteoporotic symptoms in adult male rats

“…The results by Galicka et al (2004) also demonstrate the direct toxic impact of Cd on bone collagen content in rats. Moreover, the decreased biomechanical strength of the femur was also reported in the Se-exposed rabbits (Turan et al 1997). Changes in mechanical properties and composition of bone can be also induced by various OP pesticides as a result of metabolic process disruption of bone tissue (Ware 2001).…”

Effect of vitamin C on bone formation in the expanded inter-premaxillary suture. Early bone changes

“…In an animal study, bone healing was impaired by the presence of oxygen-free radicals [8]. Antioxidant administration has been shown to be beneficial in suppressing the damaging effects of oxygen-free radicals in cells during bone healing [20,21,26]. Vitamin C deficiency has also been associated with decreased bone density in oxidans in eine orthopädisch geweitete Sutur hat jedoch negative Auswirkungen auf die Knochenbildung.…”

“…In einem Tierversuch führte das Vorliegen von freien Sauerstoffradikalen zu einer Behinderung der Knochenheilung [8]. Die Verabreichung von Antioxidantien hat sich als vorteilhaft erwiesen, um die schädlichen Auswirkungen von freien Sauerstoffradikalen in den Zellen während der Knochenheilung zu unterdrücken [20,21,26]. Darüber hinaus wurde Vitamin-C-Mangel in Tierversuchen auch mit einer geringeren Knochendichte in Verbindung gebracht [1,12,20].…”

Stimulation of bone formation in the expanding inter-premaxillary suture by vitamin E, in rat

“…16 Findings from animal studies indicate that α-tocopherol improves calcium content and mechanical properties of bone tissue. 17,18 Moreover, the USA Food and Drug Administration (FDA) classify α-tocopherol as Generally Recognized as Safe (GRAS) and it is worldwide approved for use in foods contact applications. Moreover, there are new studies that have shown the beneficial effects of α-tocopherol on bone formation.…”

“…Moreover, there are new studies that have shown the beneficial effects of α-tocopherol on bone formation. [9][10][11]17,18 Effects of α-tocopherol on long bone distraction osteogenesis (DO) also have been investigated. Therefore, to our best notice, our study is the first report to evaluate the effects of α-tocopherol on bone formation in the sutural area.…”