Effect of dietary supplementation of betaine on productive performance, egg quality and jejunal tight junction-related gene expression in laying hens raised under hot environmental conditions

Shin, J.E.; Kim, J.H.; Goo, Doyun; Han, Gi Ppeum; Pitargue, Franco Martinez; Kang, Hwan-Ku; Kil, Dong Yong

doi:10.1016/j.livsci.2018.05.013

Cited by 24 publications

(19 citation statements)

References 16 publications

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“…Decreased TER values for intestinal tissues and increased LPS concentrations in the blood represent increased intestinal permeability. Furthermore, intestinal barrier function is highly associated with the integrity of mucosal tight junction, which is a sealing protein complex between enterocytes in the GIT [6,11]. Decreased expression of tight junction-related genes and proteins in the GIT is often correlated with decreased intestinal barrier function [6,11].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, intestinal barrier function is highly associated with the integrity of mucosal tight junction, which is a sealing protein complex between enterocytes in the GIT [6,11]. Decreased expression of tight junction-related genes and proteins in the GIT is often correlated with decreased intestinal barrier function [6,11]. Thus, we measured jejunal TER values, serum LPS concentrations, and the expression of four important tight junction-related genes ( ZO-1 , OCLN , CLDN-1 , and JAM-2 ) in the jejunal mucosa.…”

Section: Discussionmentioning

confidence: 99%

“…The analysis of tight junction-related gene expression in the jejunal mucosa was performed with the method of Shin et al [6]. Briefly, total RNA was extracted from the jejunal mucosa using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, poultry exposed to HS frequently experience various physiological disturbances, such as systemic immune dysregulation, endocrine disorders, respiratory alkalosis, and electrolyte imbalance [2,3,4], which decrease poultry health and performance. In addition, one of the physiological challenges associated with these negative outcomes is an impaired intestinal barrier function [5,6]. Lambert [5] suggested that HS diverts blood flows from the gastrointestinal tract (GIT) to the skin, which damages the mucosal tight junction barrier in the GIT.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Heat Stress and Stocking Density on Growth Performance, Breast Meat Quality, and Intestinal Barrier Function in Broiler Chickens

Goo

Kim

Park

et al. 2019

Animals

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117

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The present experiment was conducted to investigate the effect of heat stress (HS) andstocking density (SD) on growth performance, breast meat quality, and intestinal barrier functionin broiler chickens. Experimental treatments included two different ambient temperatures (20 °C:thermoneutral conditions, or 27.8 °C: HS conditions) and two different SD (low: 9 birds/m2 andhigh: 18 birds/m2) in a 2 × 2 factorial arrangement. A total of 1140 21-day-old broiler chickens wereallotted 1 of 4 treatments with five replicates. At the end of the experiment (35 days of age), twobirds per replicate were euthanized for sample collections. The results indicated no interactionsbetween HS and SD for all measurements. For main effects, HS decreased (p < 0.05) the growthperformance of broiler chickens. Similarly, high SD also decreased (p < 0.05) body weight gain andfeed intake. HS decreased (p < 0.01) jejunal trans-epithelial electric resistance (TER), whereas highSD did not affect TER. Neither HS nor high SD affected jejunal tight junction-related geneexpressions; however, high SD reduced (p < 0.05) occludin expression. In conclusion, HS and highSD are key environmental factors decreasing broiler performance; however, the interactive effectsof HS and high SD are not significant under the current conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Heat Stress and Stocking Density on Growth Performance, Breast Meat Quality, and Intestinal Barrier Function in Broiler Chickens

Goo

Kim

Park

et al. 2019

Animals

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117

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“…The liver was removed and stored at −80 °C for the analysis of gene expression. The gene ( Table 2 ) mRNA expression was measured as described by Shin et al [ 22 ]. Total RNA of liver samples was extracted using Trizol reagent (Invitrogen Biotechnology Inc., Carlsbad, CA, USA) according to the manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

Comparative Effects of Flaxseed Sources on the Egg ALA Deposition and Hepatic Gene Expression in Hy-Line Brown Hens

Shahid

Raza

et al. 2020

Foods

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Healthy diets are necessary for both humans and animals, including poultry. These diets contain various nutrients for maintenance and production in laying hens. Therefore, research was undertaken to explore the efficiency of various dietary flaxseed sources on the n-3 deposition in the egg yolk and gene expression in laying hens. Five dietary groups were analyzed, i.e., (i) a corn-based diet with no flaxseed (FS) as a negative control (NC), (ii) a wheat-based diet supplemented with 10% whole FS without multi-carbohydrase enzymes (MCE) as a positive control (PC), (iii) ground FS supplemented with MCE (FS), (iv) extruded flaxseed meal was supplemented with MCE (EFM), (v) flaxseed oil supplemented with MCE (FSO). Results indicated that egg weight was highest in the NC, FS, EFM, and FSO groups as compared to PC in the 12th week. Egg mass was higher in enzyme supplemented groups as compared to the PC group, but lower than NC. In the 12th week, the HDEP (hen day egg production) was highest in the FS and EFM groups as compared to FSO, PC, and NC. The FCR (feed conversion ratio) was better in enzyme supplemented groups as compared to the PC group. Enzyme addition enhanced the egg quality as compared to PC in the 12th week. The HDL-C (high-density lipoprotein cholesterol) was increased, while LDL-C (low-density lipoprotein cholesterol), VLDL-C (very-low-density lipoprotein cholesterol), TC (total cholesterol), and TG (total triglycerides) were reduced in the enzyme supplemented groups as compared to PC and NC. The FSO deposit more n-3 PUFA and docosahexaenoic acid (DHA) in the egg yolk as compared to FS and EFM groups. The expression of ACOX1, LCPT1, FADS1, FADS2, and ELOV2 genes were upregulated, while PPAR-α was downregulated in the FSO group. The LPL mRNA expression was upregulated in the FS, EFM, and FSO groups as compared to the PC and NC groups. It was inferred that FSO with enzymes at 2.5% is cost-effective, improves the hen performances, upregulated the fatty acid metabolism and β-oxidation genes expression, and efficiently deposits optimal n-3 PUFA in the egg as per consumer’s demand.

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Nutritional status and growth performance of Fijian non-descript local chickens and their crosses with broilers under different production systems

Berukilukilu,

Bakare,

Iji

et al. 2024

Trop Anim Health Prod

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The study compared nutrient intake and growth performance of local chickens to that of local x broiler crossbreds under scavenging and indoor conventional systems. A total of 48 male and 48 female chickens for each of the two chicken types were allocated to four outdoor free-range pens. The chickens were allowed to scavenge whilst being supplemented with sorghum plus kitchen waste and broiler growers from week 5 to week 13 of age. The same design was repeated using the indoor conventional system. Local chickens and their crosses with broilers had higher growth rates under the scavenging system than the indoor production system (P < 0.05). Local chickens and their crosses with broilers had the same growth rates when fed the same diet (P > 0.05). Crop and gizzard contents from local chickens had the same crude protein as their crosses with broilers under both systems (P > 0.05). The crude protein values of crop and gizzard contents ranged from 25.4 to 30.4%. Crop and gizzard contents from scavenging chickens had energy content ranging from 16.2 to 17.1 MJ/Kg which was lower (P < 0.05) than that from chickens under the indoor conventional system (20.3 to 25.8 kJ/Kg). Iron content ranged from 655.7 to 1619.4 mg/Kg in scavenging chickens and 156.1 to 621.4 mg/Kg in enclosed chickens. Chickens of the same type had higher iron content in their crop and gizzard contents under the scavenging system than the conventional system (P < 0.05). Crossbreds between local chickens and broilers matches the scavenging abilities of the local chickens but have lower growth rates under the scavenging system.

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Effect of dietary supplementation of betaine on productive performance, egg quality and jejunal tight junction-related gene expression in laying hens raised under hot environmental conditions

Cited by 24 publications

References 16 publications

Effect of Heat Stress and Stocking Density on Growth Performance, Breast Meat Quality, and Intestinal Barrier Function in Broiler Chickens

Effect of Heat Stress and Stocking Density on Growth Performance, Breast Meat Quality, and Intestinal Barrier Function in Broiler Chickens

Comparative Effects of Flaxseed Sources on the Egg ALA Deposition and Hepatic Gene Expression in Hy-Line Brown Hens

Nutritional status and growth performance of Fijian non-descript local chickens and their crosses with broilers under different production systems

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