Effect of dietary supplementation with DHA on the quality of fresh, cooled and frozen stallion semen

Elhordoy, Daniel; Cazales, N.; Costa, Gustavo Lage; Estévez, José Jerónimo

doi:10.1016/j.anireprosci.2008.05.096

Cited by 11 publications

(6 citation statements)

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“…Isso pode explicar um pouco os resultados do presente estudo, tendo em vista que esses reprodutores foram responsáveis por dificultar a identificação de diferenças mínimas entre um ou outro diluidor. Ademais, Elhordoy et al (2008) chamaram atenção para o fato de que a suplementação oral de DHA só resultou em aumento da produção diária de espermatozoides, bem como em melhoria da qualidade seminal, diante do processo de refrigeração e congelação em garanhões com baixa qualidade de sêmen e considerados "bad coolers/bad freezers".…”

Section: Resultsunclassified

Efeito do ácido docosa-hexaenoico e do Trolox® no diluidor de refrigeração de sêmen de garanhões da raça Mangalarga Marchador

Aguiar

Barros

Machado

et al. 2020

Arq. Bras. Med. Vet. Zootec.

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RESUMO Objetivou-se avaliar o efeito do ácido docosa-hexaenoico (DHA), associado ou não ao Trolox®, na refrigeração de sêmen de garanhões da raça Mangalarga Marchador. Foram refrigerados 10 ejaculados nos diluidores: D1) BotuSêmen® (BS; controle); D2) BS + 30ngmL-1 de DHA (BS30DHA); D3) BS30DHA + 40µM de Trolox® (BS30DHA40T); D4) BS + 50ngmL-1 de DHA (BS50DHA); D5) BS50DHA + 40µM de Trolox® (BS50DHA40T). Após 48 horas de refrigeração, foram avaliados os parâmetros de movimento espermático, a integridade estrutural e funcional da membrana plasmática e a longevidade espermática. Todos os diluidores testados preservaram, de forma semelhante, a motilidade, a linearidade, a retilinearidade, a amplitude do deslocamento lateral da cabeça, a frequência do batimento flagelar cruzado, o percentual de hiperativos e a integridade estrutural e funcional da membrana espermática (P>0,05). O diluidor BS50DHA foi superior ao BS30DHA40T em preservar a VCL e a VSL e foi superior ao BS30DHA40T e ao BS50DHA40T em preservar a VAP e o índice de oscilação (P<0,05). Conclui-se que o uso do Trolox® em diluidores utilizados para refrigeração de sêmen de garanhões contendo ácido docosa-hexaenoico, nas concentrações propostas, não é indicado por alterar parâmetros de movimento espermático considerados importantes para a fertilidade.

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Section: Resultsunclassified

Efeito do ácido docosa-hexaenoico e do Trolox® no diluidor de refrigeração de sêmen de garanhões da raça Mangalarga Marchador

Aguiar

Barros

Machado

et al. 2020

Arq. Bras. Med. Vet. Zootec.

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“…There is therefore significant practical application of the results here that feeding flax seed, a source of n‐3, can improve the hardiness of sperm and lengthen the storage interval between collection and grievous loss of sperm function. On a similar but smaller scale, stored sperm from stallions fed an n‐3 FA source had better functional ability (viability and motility) than sperm from control stallions (Elhordoy et al. 2008).…”

Section: Discussionmentioning

confidence: 92%

“…2001; Mitre et al. 2004) and stallions (Elhordoy et al. 2008) although boars on n‐3 enriched diets have also shown improved sperm tail morphology with no improvement in motility (Estienne et al.…”

Section: Discussionmentioning

confidence: 99%

Stress and Dietary Factors Modify Boar Sperm for Processing

Radomil

Pettitt

Merkies

et al. 2011

Reprod Domestic Animals

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ContentsThis paper reviews stresses boar sperm undergo during processing and presents preliminary results of dietary modification that minimize this damage. Processing for artificial insemination (AI) stresses boar sperm by osmotic effects; altering cell size, shape and membranes; intracellular ice formation; and production of reactive oxygen species (ROS). Sperm response to ROS is concentration-dependent, with low levels activating the ERK pathway to stimulate tyrosine phosphorylation (Tyr-P) and capacitation, but high concentrations or inappropriately timed onset of ROS pathways can harm sperm. Fresh boar sperm exposed to ROS increased intracellular hydrogen peroxide (H 2 O 2 ) phospholipase and lipid peroxidation, maintained viability but lost motility and underwent acrosome reactions (AR). Direct incorporation of lipids ± the antioxidant Vitamin E improves the survival of liquid-and frozen-stored semen. Boars fed dietary flaxseed for 8 weeks to increase n-3 fatty acids displayed improved sperm morphology (p < 0.05), increased membrane fluidity (p < 0.05) and better retention of motility and viability during 5-7 day storage (p < 0.05). Processes reducing oxidative damage to stored sperm should be evaluated.

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“…Moreover the results obtained herein were similar to those observed by Sieme et al (2004), but 17.0% better than Sieme et al (2006) who obtained 53.1% of progressive spermatozoa motility when the semen was stored at 5°C for 24 h and 30-40% better when stallion spermatozoa was cooled during 24 or 48 h (Dawson et al, 2000). There are a number of studies to improve the motility and fertility of stallion semen after cooling and/or frozen-thawed, however, no satisfactory results have been obtained (Martin et al, 1979;Palacios and Zarco, 1996;Rugby et al, 2001;Warnke et al, 2003;Sieme et al, 2006;Deichsel et al, 2008;Elhordoy et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Effect of cooling on sperm motility before and after frozen-thawed stallion semen

Ledezma-Torres¹,

Sandoval²,

Valladares³

et al. 2014

Afr. J. Biotechnol.

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The aim of this study was to assess the effect of cooling on sperm motility before and after frozenthawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5°C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5°C for 0, 2, 7 or 24 h) after the semen was frozenthawed. Sperm progressive motility (83.1, 78.7, 74.8 or 70.3%, respectively) was significantly different (P<0.05) at different hours of cooling before freezing. Similar pattern was found when semen was subjected to cooling, frozen-thawed and cooling time resulted in a progressive reduction in motility from 39.4 to 26.9%. The motility of semen subjected only to cooling for 24 h before freezing was optimal (70.0%) for artificial insemination. Moreover, semen subjected to cooling for 7 or 24 h before and after frozen-thawed could be used still with some considerations for artificial insemination.

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Effect of dietary supplementation with DHA on the quality of fresh, cooled and frozen stallion semen

Cited by 11 publications

References 0 publications

Efeito do ácido docosa-hexaenoico e do Trolox® no diluidor de refrigeração de sêmen de garanhões da raça Mangalarga Marchador

Efeito do ácido docosa-hexaenoico e do Trolox® no diluidor de refrigeração de sêmen de garanhões da raça Mangalarga Marchador

Stress and Dietary Factors Modify Boar Sperm for Processing

Effect of cooling on sperm motility before and after frozen-thawed stallion semen

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