Effect of Dietary β-1,4-Mannobiose on the Growth of Growing Broiler Chicks

Abstract

We investigated the effects of dietary β-1,4-mannobiose (MNB) on the weights of body, breast muscles, thighs, liver, and abdominal adipose tissue in growing broiler chicks to evaluate whether MNB can be used as a feed additive for broiler chicks. A total of 24 eight day-old male broiler chicks were allocated to two cages and fed a control diet or a 0.01% MNB-supplemented diet for 14 days. Dietary MNB significantly increased the relative weight of breast muscles, whereas the weights of the body, thighs, liver and abdominal adipose tissue were not affected. The myostatin mRNA level in the breast muscle was significantly reduced by MNB. Since myostatin is a negative regulator of myoblast proliferation and differentiation, it is possible that the downregulation of myostatin gene expression is involved in the increased breast muscle growth with MNB. The plasma 3-methyl histidine level, which is known to be a nonmetabolizable amino acid marker of myofibrillar protein catabolism, and the breast muscle atrogin-1 mRNA level, which is involved in protein catabolism, were not affected by dietary MNB. In addition, MNB did not affect protease mRNA levels in the breast muscles. These results suggest that MNB does not affect proteolysis in the breast muscles. All our findings suggest that MNB is a promising candidate feed additive to improve the meat yield of broiler chickens.

Dietary mannanase‐hydrolyzed copra meal improves growth and increases muscle weights in growing broiler chickens

“…Complementary DNAs of myostatin, atrogin‐1, 20S proteasome subunit C1, 20S proteasome C2 subunit, m‐calpain large subunit, caspase 3, and cathepsin B were amplified with the primers as previously described (Ibuki et al . ). Complementary DNAs of IGF‐1 (GenBank accession no.…”

“…As an internal standard, cDNA of ribosomal protein S17 (RPS17) was also amplified with the primers as previously described (Ibuki et al . ). All primers were purchased from Hokkaido System Science Co., Ltd. (Hokkaido, Japan).…”

“…First-strand complementary DNA (cDNA) was synthesized from total RNA using a ReverTra Ace ® qPCR RT Master Mix with gDNA Remover (Toyobo Co. Ltd, Osaka, Japan) with random primers. Complementary DNAs of myostatin, atrogin-1, 20S proteasome subunit C1, 20S proteasome C2 subunit, m-calpain large subunit, caspase 3, and cathepsin B were amplified with the primers as previously described (Ibuki et al 2013). Complementary DNAs of IGF-1 (GenBank accession no.…”

“…NM_205303) were amplified with the following primers: IGF-1 sense, 5′-GCT GCC GGC CCA GAA-3′; antisense, 5′-ACG AAC TGA AGA GCA TCA ACC A-3′; IGF-2 sense, 5′-CCT GGC TCT GCT GGA AAC C-3′; antisense, 5′-GAG AGG TCA CGC TCT GAC TTG A-3′; and μ-calpain sense, 5′-CAC ACA AGG AGG CCG ACT TC-3′; antisense, 5′-TCC GCT GTG TCT GAC TGC TT-3′. As an internal standard, cDNA of ribosomal protein S17 (RPS17) was also amplified with the primers as previously described (Ibuki et al 2013). All primers were purchased from Hokkaido System Science Co., Ltd. (Hokkaido, Japan).…”

Correlation analysis of hypothalamic mRNA levels of appetite regulatory neuropeptides and several metabolic parameters in 28‐day‐old layer chickens

“…Complementary DNAs of NPY, POMC and CRF were amplified with the primers as described previously (Saneyasu et al 2013). As an internal standard, cDNA of ribosomal protein S17 was also amplified with the primers as previously described (Ibuki et al 2013). THUNDERBIRD TM SYBR ® qPCR Mix was purchased from TOYOBO CO. LTD. (Osaka, Japan), and mRNA expression was quantified in duplicate using the Applied Biosystems 7300 Real-Time PCR system according to the supplier's recommendations (Applied Biosystems, Foster City, CA, USA).…”