Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection

Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum

“…The present results showed low but significant activity when the fungus was grown on glucose as the main carbon source. In these controlled culture media, a pH decrease was confirmed, as previously reported for Sclerotinia (Cessna et al, 2000;Rollins and Dickman, 2001;Billon-Grand et al, 2012;Bueno et al, 2012). The initial pH of the inoculated media was adjusted to 5.0, and a rapid decline in pH was observed 72-96 h after transfer in all treatments (4.0-2.8; Figure 2).…”

“…The culture supplemented with citrus pectin presented the highest acidification (2.8; Figure 2). The acidification of the culture medium likely occurred owing to the secretion of oxalic acid, an important factor in S. sclerotiorum pathogenesis (Noyes and Hancock, 1981;Marciano et al, 1983;Magro et al, 1984;Godoy et al, 1990;Cessna et al, 2000;Rollins and Dickman, 2001;Billon-Grand et al, 2012;Bueno et al, 2012). Oxalic acid produced by S. sclerotiorum has been suggested to control the spatial pattern of cell wall-degrading enzyme production during pathogenesis (Marciano et al, 1983;Godoy et al, 1990).…”

“…sclerotiorum produces multiple hydrolytic enzymes, but studies have not yet established which of them are important pathogenicity factors or defined their specific roles during the infection process. We have previously shown that various carbon sources combined with acidification of the medium are important factors in the control of acid protease and aspartyl protease gene expression during S. sclerotiorum pathogenesis (Bueno et al, 2012). In this study, we aimed to elucidate how extracellular hydrolytic enzymes are induced during S. sclerotiorum pathogenesis in the dry bean Phaseolus vulgaris L. and to determine whether the mechanisms of regulation differ between in vitro and in planta models.…”

Extracellular protease activity and glucose production in isolates of net blotch pathogens differing in virulence

“…They also reported that the elimination of protease activity transformed the virulent pathogen into a non-pathogen. Bueno et al (2012) supported the involvement of protease enzyme in pathogenicity by showing the increased expression of the acid protease and aspartyl protease genes upon invasion of Phaseolus vulgaris plants with Sclerotinia sclerotiorum.…”

Correlation between the invasive fungal infection among and their blood glucose levels

“…Among the critically ill ICU patients, the proportion of fungal infection is up to 50% primarily because blood osmotic pressure increases with high BGL, and this increase inhibits the phagocytic functions of neutrophils, reduces the ability of the body to resist infection, and causes abnormalities in the immune system, thereby promoting the spread of fungal infection and adding value to deep organizations [8]. Bueno [9] investigated the situation of fungal infection among ICU patients and reported that their BGLs should be monitored. This finding also supports this paper.…”