Effect of different carbon sources on growth and glutamic acid fermentation by Brevibacterium sp.

Abstract

Experiments were carried out to find the efficiency of Brevibacterium sp. to utilize different carbon sources (glucose, fructose, sucrose, maltose, lactose, xylose and starch) for its growth and for the production of glutamic acid. Except starch, all the carbon sources supported the growth of the culture, which showed a preferential choice of growth and activity when grown on glucose as sole carbon source. Mixed substrate fermentation did not improve the yield of amino acid. Maximum glutamic acid yields (6.86 mg/ml) were obtained when 2% glucose medium was fermented under optimized conditions for 48 h.The establishment of L-glutamic acid fermentation provided a significant impetus to the development of microbial production of primary metabolites. Selection of suitable carbon source and its concentration in fermentation medium is of utmost importance in amino acid production. The production yield seems to be largely influenced by the carbon source in the culture medium (SUGIMOTO and SHUO 1989). When amino acids are produced from different carbon source, the yields are strongly affected by the metabolic pathways specific to respective carbon source and the regulatory mechanisms on the pathways (SHIIO et al. 1990). L-glutamic acid production by Brevibacteriumflavum mutant M-128 from glucose, fructose, sucrose and from the mixed substrate fermentation was not so strongly affected by these carbon sources while L-phenylalanine production was greatly affected (SHUO et al. 1990). Glutamate can also be produced from carbon sources other than sugars like, hydrocarbons (KOBAYASHI et al. This paper deals with the effect of different carbon sources on growth and glutamic acid fermentation by a strain of Brevibacterium sp. Materials and methodsOrganism and culture medium: A strain of Brevibacterium sp. DSM 20411 was used in the present studies. Culture was grown on a medium containing (gA) casein peptone 10.0, yeast extract 5.0, glucose 5.0, NaCl5.0, agar powder 15.0 and distilled water (pH 7.2-7.4). Slants were prepared and stored at 4 "C and renewed twice a month.

Modeling of glutamic acid production by Lactobacillus plantarum MNZ

“…In another study, a wide range of different carbon sources including glucose, fructose, sucrose, maltose, lactose, xylose and starch were evaluated for the highest glutamic acid production and a 2% glucose was found to be the best (Nampoothiri and Pandey, 1995b). Assessment of three different nitrogen sources (ammonium nitrate, sodium nitrate and urea) on glutamic acid production showed that ammonium nitrate (1%) was the best than the other nitrogen sources (Jyothi et al 2005).…”

“…In the last decade, various strategies have been employed to optimize glutamic acid production such as cell recycling (Ishizaki et al 1993), nutrient formulation (Nampoothiri and Pandey, 1995a), culturing the bacteria in solid substrates (Nampoothiri and Pandey, 1996a), and the use of different raw materials (Das et al 1995;Jyothi et al 2005). The optimal conditions of the fermentation process for glutamic acid production by different bacterial strains have already been reported (Nampoothiri and Pandey, 1995b;Shiratsuchi et al 1995;Delaunay et al 2002;Jyothi et al 2005). Of these factors, pH, temperature, various carbon and nitrogen sources have been evaluated.…”

Studies on Lysine Accumulation in the Broth Culture of Bacillus Species using Carbohydrates as Carbon Sources and Seed Meals as Nitrogen Sources

“…In a separate study conducted by Roy and Chatterjee (1989) they observed that 8% of glucose concentration produced the highest amount of glutamic acid respectively. Nampoothiri and Pandey (1995), reported that maximum yield of glutamic acid (6.86 mg/ml) was obtained when 2% glucose medium was fermented for 48h by a Brevibacterium species. Also, Ekwealor and Obeta (2005) in their study observed that B. megaterium SP14 recorded the highest amount of lysine when 8.0% (w/v) glucose was used as source of carbon.…”

Fermentation and recovery of L-glutamic acid from cassava starch hydrolysate by ion-exchange resin column

“…(DSM 20411) was used in the present study. The growth medium, culture preservation conditions and inoculum preparation were same as mentioned elsewhere (8).…”

“…(9). The optimum parameters obtained in earlier studies were maintained throughout the period of fermentation such as pH 7.5, temperature 30°C, and agitation speed 180 rpm (8)(9)(10). Samples were withdrawn as whole flask at desired time intervals for analysis.…”