Effect of different cell disruption methods on lipid yield of <i>Schizochytrium</i> sp.

İsa, Mustafa Hac; Metin, Cansu; Ercan, Ertan; Alparslan, Yunus

doi:10.1002/aocs.12551

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…Enzymatic hydrolysis of Chlorella vulgaris is superior to other physical breaking methods (Zheng et al, 2011). When comparing the effects of various cell disruption methods on lipid production in Schizochytrium sp., pretreatment with lysozyme and cellulase results in the highest extraction rate and highest DHA content (Hac İsa et al, 2022).…”

Section: Biochemical Wall-breaking Methodsmentioning

confidence: 99%

Microalgal polyunsaturated fatty acids: Hotspots and production techniques

Chen

et al. 2023

Front. Bioeng. Biotechnol.

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Algae play a crucial role in the earth’s primary productivity by producing not only oxygen but also a variety of high-value nutrients. One such nutrient is polyunsaturated fatty acids (PUFAs), which are accumulated in many algae and can be consumed by animals through the food chain and eventually by humans. Omega-3 and omega-6 PUFAs are essential nutrients for human and animal health. However, compared with plants and aquatic sourced PUFA, the production of PUFA-rich oil from microalgae is still in the early stages of exploration. This study has collected recent reports on algae-based PUFA production and analyzed related research hotspots and directions, including algae cultivation, lipids extraction, lipids purification, and PUFA enrichment processes. The entire technological process for the extraction, purification and enrichment of PUFA oils from algae is systemically summarized in this review, providing important guidance and technical reference for scientific research and industrialization of algae-based PUFA production.

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Section: Biochemical Wall-breaking Methodsmentioning

confidence: 99%

Microalgal polyunsaturated fatty acids: Hotspots and production techniques

Chen

et al. 2023

Front. Bioeng. Biotechnol.

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“…It is essential to disintegrate the cells before lipid extraction to improve the mass transfer to the extraction solvent. Several physical, mechanical, chemical, and biological (enzymatic) pretreatments are utilized to disrupt the rigid cell wall of oleaginous biomass [9,18,19]. Specifically, the complex and rigid cell wall of microalgae hinders solvent penetration, resulting in low extraction of lipids [9].…”

Section: Pretreatments Before Extractionmentioning

confidence: 99%

Advances in Lipid Extraction Methods—A Review

Saini

Prasad

Shang

et al. 2021

IJMS

192

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Extraction of lipids from biological tissues is a crucial step in lipid analysis. The selection of appropriate solvent is the most critical factor in the efficient extraction of lipids. A mixture of polar (to disrupt the protein-lipid complexes) and nonpolar (to dissolve the neutral lipids) solvents are precisely selected to extract lipids efficiently. In addition, the disintegration of complex and rigid cell-wall of plants, fungi, and microalgal cells by various mechanical, chemical, and enzymatic treatments facilitate the solvent penetration and extraction of lipids. This review discusses the chloroform/methanol-based classical lipid extraction methods and modern modifications of these methods in terms of using healthy and environmentally safe solvents and rapid single-step extraction. At the same time, some adaptations were made to recover the specific lipids. In addition, the high throughput lipid extraction methodologies used for liquid chromatography-mass spectrometry (LC-MS)-based plant and animal lipidomics were discussed. The advantages and disadvantages of various pretreatments and extraction methods were also illustrated. Moreover, the emerging green solvents-based lipid extraction method, including supercritical CO2 extraction (SCE), is also discussed.

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“…contains 31 -68% of lipid and Schizochytrium sp. contains 50 -77% of lipid [14][15][16][17] . Microalgae produce and store neutral lipids as unsaturated fats, phospholipids, glycolipids, and it can be utilized as feedstocks for biodiesel production 18 .…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Lipid and Biomass Production in Microalgae Scenedesmus abundans by Microwave Irradiation

Kapavarap¹,

Kotikalapudi²,

Oruganti³

et al. 2022

Int J Life Sci Pharm Res

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Due to the rising depletion of fossil fuels and increased energy demands, human society is looking for clean sustainable energy. Commercially produced algal biodiesel is limited by the expense and difficulty of oil extraction and subsequent biodiesel conversion technologies. Microalgae with high oil content are only alternatives for decreasing fossil fuel supplies, but more work remains to be done to improve the lipid content of microalgae strains. In this study, strain improvement is done using microwave radiation in Scenedesmus abundans to increase the production of triacylglycerol, which is the main source of biodiesel. Microalgal cultures were exposed to varied microwave irradiation over different time periods. Under microwave irradiation, 20-25 mins reaction time seems suitable for the complete in situ transesterification reaction. Microwave heating transesterification has been shown to be more effective for adequate biodiesel yield compared to the conventional transesterification process. Maximum increase of 2.22-fold in biomass, and 2.5-fold in triacylglycerol was observed for microwave irradiation of 25 mins and 20 mins intervals respectively. The percentage of some monounsaturated fatty acids increased in gas chromatographic examination of neutral lipid fractions from total lipids of microwave irradiated samples, is considered as one of the preferable properties of biodiesel. According to our study findings, Scenedesmus abundans qualifies as the most efficient feedstock for biodiesel production, and microwave-assisted in situ transesterification reduces the requirement for a large amount of solvents, longer reaction times, and high reaction temperatures and pressures.

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Effect of different cell disruption methods on lipid yield of Schizochytrium sp.

Cited by 9 publications

References 36 publications

Microalgal polyunsaturated fatty acids: Hotspots and production techniques

Microalgal polyunsaturated fatty acids: Hotspots and production techniques

Advances in Lipid Extraction Methods—A Review

Enhancement of Lipid and Biomass Production in Microalgae Scenedesmus abundans by Microwave Irradiation

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