Effect of different chitosan derivatives on in vitro scratch wound assay: A comparative study

Felice, Francesca; Zambito, Ylenia; Belardinelli, Ester; Fabiano, Angela; Santoni, Tatiana; Stefano, Rossella Di

doi:10.1016/j.ijbiomac.2015.02.041

Cited by 109 publications

(76 citation statements)

References 38 publications

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“…Chlorhexidine (CHX) is a bis-guanide antiseptic that displays a wide range of antimicrobial activity, in addition to its safety, effectiveness, substantivity and low toxicity (5). CHX now comes in many different forms, including mouthwashes, bioadhesive gels, dental varnishes, and chips (1,7).…”

Section: Discussionmentioning

confidence: 99%

“…Cicatrization is a physiological process through which the loss of integrity of oral mucosa is recovered and damaged tissues are repaired (5). The process begins with hemostasis and leads to three dynamic, interrelated phases: the inflammatory, proliferative and remodeling phases (5,6).…”

Section: Introductionmentioning

confidence: 99%

“…The process begins with hemostasis and leads to three dynamic, interrelated phases: the inflammatory, proliferative and remodeling phases (5,6). These involve cell migration and transmigration, vasoconstriction, vasodilatation, angiogenesis, granulation tissue formation and extracellular matrix deposition.…”

Section: Introductionmentioning

confidence: 99%

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The effects of a topical gel containing chitosan, 0,2% chlorhexidine, allantoin and despanthenol on the wound healing process subsequent to impacted lower third molar extraction

Madrazo-Jiménez¹,

Rodríguez-Caballero²,

Serrera-Figallo³

et al. 2016

Med Oral

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BackgroundDespite efforts to prevent postoperative discomfort, there are still many immediate side effects associated with the surgical extraction of impacted lower third molars. Cicatrization is a physiological process through which the loss of integrity of oral mucosa is recovered and damaged tissues are repaired. Bexident Post (ISDIN, Spain) is a topical gel that contains chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol. While this gel has many clinical indications, there are no published clinical trials evaluating its use in impacted mandibular third molar surgery. This study aims to clinically evaluate the efficacy of a gel containing chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol on wound healing and reduction of postoperative side effects and complications after extraction of an impacted mandibular third molar.Material and MethodsA split-mouth design study was carried out on a total of 50 bilaterally and symmetrically impacted third molar extractions, which were randomly placed into either a control group (CG=25) or an experimental group (EG=25). Patients were all informed of the purpose of the study and provided written consent. All procedures were carried out by the same dental practitioner, in accordance with standard surgical protocol. A different dental practitioner, unaware of which treatment had been applied, provided follow-up care. The EG applied 10 ml of topical gel composed of chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol to the surgical wound three times a day for 10 days, patients in the CG did not apply any gel.ResultsThe groups were homogeneous insofar as potentially confounding variables. No significant findings were found regarding postoperative swelling and pain. Neither of the groups displayed poor healing or infectious complications of the wound during the postoperative period. In all the recorded follow-ups (Day 7 p=0.001, and Day 14 p=0.01), the wound’s aesthetic appearance was better in the EG. Overall treatment tolerance was satisfactory and similar in both groups.ConclusionsThe gel composed of chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol did not aid in patients’ postoperative comfort; however, improved wound healing was observed. Key words:Impacted lower third molar, postoperative wound healing, chitosan, chlorhexidine, allantoin, dexpanthenol, postoperative period.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effects of a topical gel containing chitosan, 0,2% chlorhexidine, allantoin and despanthenol on the wound healing process subsequent to impacted lower third molar extraction

Madrazo-Jiménez¹,

Rodríguez-Caballero²,

Serrera-Figallo³

et al. 2016

Med Oral

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“…Subsequently, the cell debris was removed with pipettes, and a washing procedure with serum-free medium was carried out. DMSO (solvent control) and 0 (zero control), 0.5, 5.0, 50.0, and 100.0 µmol/L MMF were added to the cultures, then incubated for 48 h, and photographed at 0, 2, 4, 8, 12, 24, and 48 h. In the next step, image analysis was carried out [49]. …”

Section: Methodsmentioning

confidence: 99%

Ex vivo Cutaneous Bioavailability of Topical Mometasone Furoate in an O/W Preparation

Wohlrab

Neubert²,

Sommer³

et al. 2016

Skin Pharmacol Physiol

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Mometasone furoate (MMF) is a modern glucocorticoid of the 4th generation, which has been proven not only for inhalation but also for cutaneous treatment. Due to its lipophilic character, it is mainly used in ointments and creams with an outer lipophilic phase (W/O type). However, this study investigated the cutaneous cytotoxicology of MMF and tried to characterize its pharmacokinetic effects on the skin using an O/W preparation. An HPLC method has been developed and validated for the detection of MMF in cutaneous tissue, and concentration-time curves of MMF were created after cutaneous application on unaffected as well as lesional skin. Cytotoxicological characterization was carried out using scratch assays on keratinocytes and cutaneous fibroblasts. Results showed that the condition of the skin had no significant impact on the cutaneous bioavailability of MMF, but the intrinsic effect of the O/W vehicle could be utilized in periods of acute inflammation. Cytotoxicological data gave no new indications regarding the safety of MMF.

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“…The static imaging modality was used in preliminary dose-response experiments, to select the most effective dose/s to be used in the time-lapse experiments. In the static imaging modality, the closure of the scratch was quantified, as recently described (Felice et al, 2015), by measuring the difference between the wound width at T0 and T24, using the ImageJ processing software [http://rsbweb.nih.gov/ij/]. Scratch Closure Rate (SCR) was calculated as described by Felice et al, 2015 using the following formula:…”

Section: In Vitro Scratch Wound-healing Assaymentioning

confidence: 99%

In vitro evaluation of the wound healing activity of Drypetes klainei stem bark extracts

Brusotti

Andreola

Sferrazza

et al. 2015

Journal of Ethnopharmacology

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Effect of different chitosan derivatives on in vitro scratch wound assay: A comparative study

Cited by 109 publications

References 38 publications

The effects of a topical gel containing chitosan, 0,2% chlorhexidine, allantoin and despanthenol on the wound healing process subsequent to impacted lower third molar extraction

The effects of a topical gel containing chitosan, 0,2% chlorhexidine, allantoin and despanthenol on the wound healing process subsequent to impacted lower third molar extraction

Ex vivo Cutaneous Bioavailability of Topical Mometasone Furoate in an O/W Preparation

In vitro evaluation of the wound healing activity of Drypetes klainei stem bark extracts

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