Effect of different concentrations of amino acids in human serum and follicular fluid on the development of one-cell mouse embryos in vitro

Nakazawa, Teruki; Ohashi, Kazutomo; Yamada, Mitsutoshi; Shinoda, S; Saji, F.; Murata, Yasutaka; Araki, Hiromasa

doi:10.1530/jrf.0.1110327

Cited by 34 publications

(23 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, pyruvate and glucose support high levels of maturation, and amino acids aid pronuclear formation in fertilised oocytes (Donahue & Stern 1968, Lim et al 1999. Nutrient concentrations are non-physiological in mammalian IVM systems (Nakazawa et al 1997); improvements in oocyte energy metabolism and developmental potential may be achieved by modelling nutrient profiles on those of preovulatory follicular fluid (FF) (Krisher & Bavister 1998, Smitz & Cortvrindt 1999.…”

Section: Introductionmentioning

confidence: 99%

Fluctuations in bovine ovarian follicular fluid composition throughout the oestrous cycle

Orsi

Gopichandran²,

Leese³

et al. 2005

Reproduction

View full text Add to dashboard Cite

Bovine oocyte maturation in vitro frequently results in abnormal cytoplasmic maturation and failure to acquire developmental competence. This is, in part, likely to be due to the non-physiological nutritional milieu to which oocytes are exposed. Improvements in oocyte developmental potential may be achieved by modelling nutrient profiles on those of preovulatory follicular fluid (FF). However, little is known about fluctuations in FF nutrient levels according to follicle dominance and oestrous cyclicity. This study therefore characterised the carbohydrate and amino acid profile of FF according to these parameters, and compared preovulatory FF composition with that of maturation medium. Carbohydrate concentrations (n 5 121) were determined enzymatically whilst amino acid profiles (n 5 40) were determined by reverse-phase HPLC. Pyruvate and glucose concentrations were unaffected by follicle dominance, whereas Stage III -IV lactate profiles were higher in non-dominant FF (P < 0.01). While most dominant FF amino acid concentrations were affected by oestrous stage, only glutamate, alanine, leucine and lysine levels fluctuated in non-dominant FF. Glucose and lactate concentrations were significantly negatively correlated, whereas most amino acids were significantly positively correlated with each other. Maturation medium had higher pyruvate and lower lactate concentrations than preovulatory FF (P < 0.001), whereas glucose level was similar. All amino acid levels (except histidine, taurine, alanine and tryptophan) differed significantly between maturation medium and preovulatory FF. These data indicated that FF composition varies throughout the oestrous cycle. Preovulatory FF nutrient profile differed from that of maturation medium, perhaps accounting for the poor developmental competence of in vitro matured oocytes. These data may contribute to the formulation of a nutritionally more physiological maturation medium.

show abstract

Section: Introductionmentioning

confidence: 99%

Fluctuations in bovine ovarian follicular fluid composition throughout the oestrous cycle

Orsi

Gopichandran²,

Leese³

et al. 2005

Reproduction

View full text Add to dashboard Cite

show abstract

“…It has also been found to decrease blastocyst cell numbers, reduce implantation rates, retard fetal growth and increase the incidence of exencephaly after transfer of cultured embryos into the uteri of surrogate mothers [8]. The build-up of ammonium is principally derived from the spontaneous breakdown of glutamine under conditions used for the culture of preimplantation embryos [9]. Several groups of researchers have also suggested that ammonium in media has a deleterious effect on the mouse fetus after embryo transfer [10][11][12].…”

mentioning

confidence: 99%

Medium without Ammonium Accumulation Supports the Developmental Competence of Human Embryos

Hashimoto¹,

Nishihara²,

Murata³

et al. 2008

J. Reprod. Dev.

View full text Add to dashboard Cite

Abstract. L-Glutamine has been shown to play an important role during in vitro culture of mammalian embryos. However, it is easily decomposed into ammonium, which is believed to have deleterious effects on preimplantation embryos. In this study, we assessed prospectively the developmental competence of human embryos cultured in medium containing L-glutamine or a novel stable glutamine derivative and vitamins. The subjects of this study were 41 women who underwent IVF/ET treatment from September to November 2006 and from whom 6 or more oocytes were retrieved. Sibling oocytes were randomly divided into EA/BA (EmbryoAssyst ® and BlastAssyst ® containing a novel stable glutamine derivative and vitamins), and BAS groups (BlastAssyst ® system containing L-glutamine). There was no difference in pronuclear formation rate between EA/BA and BAS (74 vs. 69%). The blastulation rates of embryos based on the number of zygotes cultured in EA/BA on Days 5 (Day 0=insemination, 54%) and 6 (63%) were significantly higher (P<0.05) than those cultured in BAS (Day 5: 33% and Day 6: 45%, respectively). The present data indicate that a medium containing a novel stable glutamine derivative and vitamins supports the developmental competence of human embryos.

show abstract

“…It was thought to be an energy substrate in place of glucose in early stages to support the embryo's development through a critical period when lactate and pyruvate fail to supply enough energy (Reitzer et al 1979, Chatot et al 1989, 1990, and may enhance the nucleotide pool as an important amino acid that can be used to synthesize purine and pyrimidine nucleotides critical for rapidly division (Salzman et al 1958, Nomura & Rubin 1988). However, Gln was shown to be the principal contributor to the toxic breakdown of amino acids that affect in vitro culturing of preimplantation embryos (Gardner & Lane 1993, Nakazawa et al 1997, Summers & Biggers 2003. Gln-free medium could lead to increases in the numbers of cells that developed in the ICM and TE (Lawitts & Biggers 1993).…”

Section: Discussionmentioning

confidence: 99%

A modified culture method significantly improves the development of mouse somatic cell nuclear transfer embryos

Dai¹,

Hao²,

Zhou³

2009

Reproduction

View full text Add to dashboard Cite

Many strategies have been established to improve the efficiency of somatic cell nuclear transfer (SCNT), but relatively few focused on improving culture conditions. The effect of different culture media on preimplantation development of mouse nuclear transfer embryos was investigated. A modified sequential media method, named D media (M16/KSOM and CZB-EG/KSOM), was successfully established that significantly improves SCNT embryo development. Our result demonstrated that while lacking any adverse effect on in vivo fertilized embryos, the D media dramatically improves the blastocyst development of SCNT embryos compared with other commonly used media, including KSOM, M16, CZB, and aMEM. Specifically, the rate of blastocyst formation was 62.3% for D1 (M16/KSOM) versus 10-30% for the other media. An analysis of media components indicated that removing EDTA and glutamine from the media can be beneficial for early SCNT embryo development. Our results suggest that in vitro culture environment plays an important role in somatic cell reprogramming, and D media represent the most efficient culture method reported to date to support mouse SCNT early embryo development in vitro.

show abstract

Effect of different concentrations of amino acids in human serum and follicular fluid on the development of one-cell mouse embryos in vitro

Cited by 34 publications

References 0 publications

Fluctuations in bovine ovarian follicular fluid composition throughout the oestrous cycle

Fluctuations in bovine ovarian follicular fluid composition throughout the oestrous cycle

Medium without Ammonium Accumulation Supports the Developmental Competence of Human Embryos

A modified culture method significantly improves the development of mouse somatic cell nuclear transfer embryos

Contact Info

Product

Resources

About