Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

Tarig, A. A.; Wahid, H.; Rosnina, Y.; Yimer, Nurhusien; Goh, Yong Meng; Baiee, Falah; Khumran, A.M.; Hammadi, Salman; Assi, Mohammed Abdulrazzaq; Ebrahimi, Mahdi

doi:10.14202/vetworld.2017.672-678

Cited by 21 publications

(24 citation statements)

References 36 publications

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“…E. longifolia Jack effect as an aphrodisiac by consumption was earlier described [ 52 ]. Soybean and egg-yolk based extender has long been established in semen cryopreservation [ 53 ]. Study in Eurycoma longifolia has been initiated and revealed cryopreservative effect in bull spermatozoa [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Semen characteristics, extension, and cryopreservation of Rusa deer (Rusa timorensis)

et al. 2017

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Aim:The objective of this research is to report parameters for breeding soundness evaluation, semen extension, and cryopreservation in Rusa timorensis.Materials and Methods:Seven healthy stags were chosen for semen collection using an electroejaculator. The collections were performed twice in a breeding season between February and June 2016. Samples were collected between 2 and 3 weeks interval, collected twice for each animal. Semen was evaluated, extended, and cryopreserved using four different extenders; Andromed®, BioXcell®, Triladyl®, and a modified Tris-egg yolk combined with Eurycoma longifolia Jack.Results:R. timorensis semen characteristics according to volume (ml), color, sperm concentration (106/ml), general motility (%), progressive motility (%), and % morphology of normal spermatozoa are 0.86±0.18 ml, thin milky to milky, 1194.2±346.1 106/ml, 82.9±2.8%, 76.1±4.8%, and 83.9±4.8%, respectively.Conclusion:Semen characteristics of R. timorensis collected by electroejaculation is good allowing for cryopreservation and future artificial insemination work. The most suitable extender for Rusa deer semen is Andromed®.

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Section: Discussionmentioning

confidence: 99%

Semen characteristics, extension, and cryopreservation of Rusa deer (Rusa timorensis)

et al. 2017

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“…The procedure was carried out as described by Tarig et al. (). In this procedure, semen samples were diluted at room temperature in a two‐step process with a Tris‐based extender composed of two fractions containing 7% glycerol.…”

Section: Methodsmentioning

confidence: 99%

“…In slow freezing, the temperature is gradually reduced in a stepwise manner during cryopreservation process (Mphaphathi, Luseba, Sutherland, & Nedambale, 2012). The procedure was carried out as described by Tarig et al (2017). In this procedure, semen samples were diluted at room temperature in a two-step process with a Trisbased extender composed of two fractions containing 7% glycerol.…”

Section: Slow Freezingmentioning

confidence: 99%

Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender

Igbokwe

Iyasere

Sobayo³

et al. 2019

Reprod Domestic Animals

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Contents Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk (TNM) extender was examined in this study. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris‐based extenders containing different levels of TNM (0, 5, 10, 15 and 20 ml/100 ml extender). The diluted semen samples were subjected to slow and rapid freezing for a period of 7 days and thereafter evaluated for sperm functional attributes (percentage motility, acrosome integrity, membrane integrity, abnormality and livability) and oxidative stress (malondialdehyde [MDA] concentration and acrosin activity) parameters. Results showed that higher (p < 0.05) motility, livability, membrane and acrosome integrities in semen cryopreserved with slow freezing compared to rapid freezing. These parameters (motility, livability and membrane integrity) were higher (p < 0.05) in semen cryopreserved with 15% TNM in both slow and rapid freezing protocols. The results revealed that semen cryopreserved in slow freezing had lower (p < 0.05) abnormality compared to rapid freezing. Acrosin activity was higher in slow freezing compared to rapid freezing. Acrosin activity was higher at 15% TNM in both slow and rapid freezing. Lower (p < 0.05) MDA concentration was observed in semen cryopreserved using slow freezing compared to rapid freezing. The findings revealed improved post‐thaw sperm functional attributes and oxidative stress parameters of WAD goat spermatozoa cryopreserved with 15% TNM using slow freezing.

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“…Nilai MPU semen sapi bali dalam penelitian ini (60-68%) berbeda dengan nilai MPU sapi Jantan di Malaysia (59%-72%) (Tarig et al, 2017). Perbedaan ini kemungkinan disebabkan oleh jenis pengencer dan jenis ternak yang berbeda, serta teknik penampungan yang digunakan berbeda (Arifiantini & Purwantara, 2010).…”

Section: Membran Plasma Utuh (Mpu)unclassified

Viabilitas Spermatozoa Sapi Bali Menggunakan Pengencer Andromed dengan Penambahan Konsentrasi Sari Wortel yang Berbeda

Yendraliza¹,

Musyrifin²,

Elviriadi³

et al. 2019

jitro

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ABSTRAK Penggunaan andromed pada waktu tertentu sulit untuk didapatkan sehingga perlu alternatif lain untuk mengurangi penggunaan andromed. Tujuan penelitian untuk mengevaluasi pengaruh sari wortel sebagai bahan pengencer mengurangi penggunaan pengencer andromed pada semen sapi bali terhadap kualitas spermatozoa sapi bali. Penelitian ini menggunakan semen sapi bali dari Balai Inseminasi Buatan Tuah Sakato, Payakumbuh. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) 4 perlakuan dengan 5 ulangan. Perlakuan pertama: 100% pengencer andromed (P0), sari wortel 5% + andromed 95% (P1), 10% sari wortel + 90% andromed (P2), 15% sari wortel + 85% andromed (P3). Peubah yang dievaluasi adalah persentase motilitas, persentase viabilitas, persentase abnormalitas dan persentase membran plasma utuh setelah pengenceran dan setelah thawing. Hasil penelitian memperlihatkan bahwa nilai motilitas dan membran plasma utuh semen sapi setelah pengenceran dan setelah thawing tidak berbeda nyata, tetapi berbeda nyata pada nilai abnormalitas dan nilai viabilitas. Kesimpulan: Sari worteldapat mengurangi pemakaian andromed sampai 15% sebagai bahan pengencer pada semen sapi bali.Kata Kunci: membran plasma, motilitas, sapi bali, spermatozoa, viabilitasABSTRACTAndromed is difficult to obtain at any given time so we need other alternatives to reduce the use of andromed. The aim of this study was to understand the effect of carrot juice as a diluent to reduce the use of andromed diluents in bali cattle semen. This study used semen from bali cattle from the Artificial Insemination Center of Tuah Sakato, Payakumbuh. The design used was completely randomized design (CRD) with four treatments and five replications. The treatments consisted of 100% andromed (P0), 5% carrot juice + 95%andromed(P1),10% carrot juice + 90% andromed (P2), and 15% carrot juice + 85% andromed (P3). The variables measured were the percentage of motility, the percentage of viability, the percentage of abnormality and percentage of intact plasma membrane after dilution and after thawing. The results showed that the motility values and membrane values of the intact plasma membrane of cattle spermatozoa after dilution and post-thawing were not significantly different, but were significantly different in abnormality percentage and viability percentage. This study concluded that carrot juice could reduce the use of andromed up to 15% as a diluent in bali cattle semen.Keywords: bali cattle, motility, plasm membrane, viability, spermatozoa

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Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

Cited by 21 publications

References 36 publications

Semen characteristics, extension, and cryopreservation of Rusa deer (Rusa timorensis)

Semen characteristics, extension, and cryopreservation of Rusa deer (Rusa timorensis)

Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender

Viabilitas Spermatozoa Sapi Bali Menggunakan Pengencer Andromed dengan Penambahan Konsentrasi Sari Wortel yang Berbeda

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