Effect of different cryo-devices on in vitro maturation and development of vitrified-warmed immature buffalo oocytes

Mahesh, Yelisetti Uma; Gibence, Henderson Rose Winnie; Shivaji, Sisinthy; Rao, Brahmasani Sambasiva

doi:10.1016/j.cryobiol.2017.01.004

Cited by 10 publications

(6 citation statements)

References 74 publications

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“…However, IVM is challenging for cryopreserved immature COCs due to membrane damage to CCs [12] and their physical/functional detachment from the oocyte, caused by transzonal projections' sensitivity to cryoprotectants and low temperatures [12,13]. In the last 20 years, the vitrification/warming of immature COCs from adult subjects has been applied to several domestic large (sheep [12,[14][15][16][17][18][19][20][21][22], cow [23][24][25][26][27], buffalo [28][29][30], goat [31], pig [32][33][34][35], horse [36][37][38][39][40][41], donkey [42]) and small (cat [43][44][45][46][47][48], dog [49]) animal species, with various results, depending on intrinsic species-specific oocyte features. Although the results of IVM, embryo development, and blastocyst rate following immature COC vitrification are still less satisfactory compared to fresh COCs, live births have been reported in bovine [50], swine [33], equine [38,…”

Section: Introductionmentioning

confidence: 99%

New Strategies for Conservation of Gentile di Puglia Sheep Breed, an Autochthonous Capital of Millennial Tradition in Southern Italy

Temerario

Monaco

Mastrorocco

et al. 2023

Animals

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Gentile di Puglia (GdP) is an autochthonous sheep breed of Southern Italy included among ovine breeds threatened by genetic erosion and extinction risk, which have been given attention by local and international institutions, thus emphasizing the need for germplasm conservation actions. In the present study, two assisted reproduction approaches, finalized for GdP conservation, were performed: (1) on-farm reproductive efficiency evaluation, expressed as pregnancy rate (PR), twin pregnancy rate (tPR), and body condition score (BCS), for three consecutive breeding cycles and (2) pre-pubertal lambs’ immature cumulus–oocyte complex (COC) retrieval, vitrification, in vitro maturation (IVM), and assessment of meiotic stage and bioenergetic-oxidative status compared with those of other Italian and European commercial breeds. PR and tPR were progressively reduced over time. In all clinical examination times, BCS was significantly lower in nonpregnant ewes compared with pregnant ones. Fresh GdP pre-pubertal lamb COCs achieved meiotic maturation and showed healthy bioenergetic–oxidative status after IVM. Vitrification reduced the oocyte maturation rate in all groups. However, mature oocytes retained their cytoplasmic maturity, expressed as a mitochondria distribution pattern and activity, indicating promising developmental competence. In conclusion, clinical- and biotechnological-assisted reproduction approaches can support conservation strategies of GdP and other local sheep breeds in Southern Italy.

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Section: Introductionmentioning

confidence: 99%

New Strategies for Conservation of Gentile di Puglia Sheep Breed, an Autochthonous Capital of Millennial Tradition in Southern Italy

Temerario

Monaco

Mastrorocco

et al. 2023

Animals

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“…Buffaloes are economically important animals in many Asian, Mediterranean, Latin American, and European countries because they are important source of milk and meat (Ondho et al 2020;Bertoni et al 2021). However, the reproductive performance of buffaloes is low due to delayed onset of puberty, silent estrus, poor conception rates, low numbers of Graafian follicles on ovaries, and poor response to hormonal stimulation, which decreases the yield of embryos in embryo transfer practices (Mahesh et al 2017). This has resulted in increased attention to in vitro embryo production (IVEP) technologies for the rapid proliferation of superior traits in this species (Gasparrini 2013).…”

Section: Introductionmentioning

confidence: 99%

Effect of in Vitro Maturation Medium Supplementation with Chitosan Nanoparticles on The River Buffalo Cumulus-Oocyte Complexes

2022

Int J Vet Sci

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The present study aimed to investigate the possible effects of chitosan nanoparticles (CNPs) supplementation to the in vitro maturation medium on the expansion of cumulus cells, nuclear maturation, and relative gene expression of Superoxide dismutase-1 (SOD1), B-cell lymphoma-2 Apoptosis Regulator (BCL2), and BCL2 Associated X (BAX) in the River buffalo. Slaughterhouse-derived cumulus-oocyte complexes (COCs) became matured in vitro in the absence (control) or presence of CNPs (10, 25, and 50µg/mL). At the end of maturation, we assessed expansion rates and denuded COCs; then, some replicates were fixed and stained to determine nuclear maturation. Other replicates were vitrified until we acquired enough oocytes, thawed, and assessed gene expression. We concluded that 10µg/mL CNPs significantly increased cumulus cell expansion and nuclear maturation. Whereas 10 and 25µg/mL CNPs non-significantly increased SOD1 relative expression, BCL2/BAX ratio for 10µg/mL CNPs was significantly higher than in control, 25 and 50µg/mL CNPs groups.

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“…In the vitrification method, a cryo-device is required to hold the sample materials and be used for submerging into liquid nitrogen. A comparative study on cryo-devices has been done by Mahesh et al [10]. They collected COCs from buffaloes and vitrified using either a conventional straw, open pulled straw, cryoloop, hemistraw or cryotop.…”

Section: Introductionmentioning

confidence: 99%

Effect of Polyvinylpyrrolidone on Vitrification of Buffalo (Bubalus bubalis) Oocytes

Bari¹,

Islam²,

Alam³

et al. 2020

J. Buffalo Sci.

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Vitrification, a method of rapid cooling, is an alternate cryopreservation method of oocytes and embryos. The present study was aimed to examine the effect of polyvinylpyrrolidone (PVP) on vitrification of buffalo oocytes. Cumulus oocyte complexes (COCs) with fully grown oocytes (120-130 µm in diameter) were aspirated from slaughtered buffalo ovaries for vitrification. COCs were treated with equilibration solution at room temperature for 5 min and then transferred to a vitrification solution for 1 min. Then the COCs were submerged into liquid nitrogen (-196C) for a while using cryotops. The COCs were thawed, diluted, and washed in a washing solution for 5 min, respectively. Vitrified oocytes were incubated for in vitro maturation (IVM) at 38.5C under an atmosphere of 5% CO2 in the air for 24 hrs. Cumulus cells surrounding the oocytes were removed mechanically, oocytes were fixed in acetic acid and ethanol, and stained with aceto-orcein to examine the meiotic stages of oocytes. The numbers of morphologically normal oocytes after vitrification were higher in 5% PVP than 0 and 10% PVP groups. A proportion of oocytes treated with 5% PVP reached the metaphase II (MII) stage while none of the oocytes from 0% and 10% PVP groupsdeveloped beyond anaphase I and metaphase I (MI) stages, respectively. These results show that PVP can be used as a cryoprotectant for the vitrification of buffalo oocytes.

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Effect of different cryo-devices on in vitro maturation and development of vitrified-warmed immature buffalo oocytes

Cited by 10 publications

References 74 publications

New Strategies for Conservation of Gentile di Puglia Sheep Breed, an Autochthonous Capital of Millennial Tradition in Southern Italy

New Strategies for Conservation of Gentile di Puglia Sheep Breed, an Autochthonous Capital of Millennial Tradition in Southern Italy

Effect of in Vitro Maturation Medium Supplementation with Chitosan Nanoparticles on The River Buffalo Cumulus-Oocyte Complexes

Effect of Polyvinylpyrrolidone on Vitrification of Buffalo (Bubalus bubalis) Oocytes

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