Effect of Different Disinfecting Agents on Dental Impressions Contaminated with Candida albicans

Abstract

Introduction: This research was carried out to compare the effects of 0.525% and 5.25% NaOCl, 2% H2O2, and 16-mg/mL propolis on additional silicone impression materials contaminated with Candida albicans at 5- and 10-minute exposure times, to evaluate the hypotheses of this study that propolis can be used for disinfecting of dental impressions. Materials and methods: A total of 122 silicone impressions were prepared. Two samples were considered the negative controls, and the rest were contaminated with C. albicans fungal species and disinfected with 0.525% NaOCl, 5.25% NaOCl, 2% H2O2, 16-mg/mL propolis, and 96% ethyl alcohol for 5 and 10 minutes (10 samples for each interval in each group). The data were analyzed with Kruskal–Wallis and Mann–Whitney U tests in all the groups at 5- and 10-minute intervals. Results: All the disinfecting agents significantly decreased C. albicans colony counts at both intervals compared to the control groups (P = 0.00). The differences in the disinfecting effects were significant between the four study groups (P = 0.00). The most significant effects were related to NaOCl at both intervals and concentrations and 2% H2O2 at the 10-minute interval (P = 0.001), followed by 2% H2O2 at the 5-minute interval and propolis (P = 0.001). Concerning propolis (P = 0.001) and 2% H2O2 (P = 0.004), the effect of exposure time was significant. Conclusion: All the disinfecting agents in the present study can be used to disinfect impressions contaminated with C. albicans; however, concerning propolis, although it resulted in significant decreases in C. albicans colony counts, the elimination of the remaining colonies was ineffective.

Comparative evaluation of the dimensional accuracy of two commercially available extended pour alginates and a conventional alginate impression material at various time intervals – An in vitro study

“…Despite the manufacturers' claims of producing accurate casts over time, the previous studies on extended pour alginates provided mixed results. 7,[15][16][17] Also, there is scarcity of data on the locally available brands of extended pour alginates. Hence, the present study was designed to evaluate and compare the dimensional accuracy of casts obtained from two extended pour alginates (Cavex ColorChange and Algeniux) and a conventional alginate (Zelgan) at extended storage periods.…”

Evaluation of ethanol and EDTA concentrations in the expression of biofilm-producing smf-1, rpfF genes in XDR clinical isolates of Stenotrophomonas maltophilia

“…In hospitals, treatment of infection due to S. maltophilia is difficult because it is naturally resistant to different classes of antibiotics, as a result, it is prominent necessary to develop novel drugs and detergents to combat this bacterium [ 33 ], and other bacteria [ 10 , 34 ]. In evaluating the power of biofilm formation in different dilutions of ethanol and EDTA, when the isolates were not exposed to ethanol and EDTA, they formed a strong biofilm.…”

Effect of propolis on the mechanophysical properties of addition silicon dental impression material

“…It was kept in airtight, clean, dark containers. 23,24 Preparation of impression specimens for linear dimensional change Putty Soft Addition Silicon Impression Materials (Zhermack, DC, Germany) were utilized To prepare the test impression specimens. Apparatus was created using the software program 3D max 2022 (1) and then printed using a 3D printer according to ISO 4823:2015 standard for testing elastomeric impression materials.…”