Effect of different doses of hexavalent chromium on mandibular growth and tooth eruption in juvenile Wistar rats

Lucca, R.C. De; Dutrey, Patricio L.; Villarino, Mariano E; Ubios, A. M.

doi:10.1016/j.etp.2008.10.001

Cited by 14 publications

(7 citation statements)

References 29 publications

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“…What is more, chromium sulfate definitely decreased residual parameters of body compositions, i.e lean mass, fat mass and soft tissue mass, in all the experimental groups. These results are in line with those of De Lucca et al [12], who also observed the decrease of body mass during a 10-day experimental administration of hexavalent chromium to Wistar rats, in 6.25 and 12.5 mg/kg BW doses. A similar effect was observed by Zha et al [38].…”

Section: Discussionsupporting

confidence: 82%

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The peripheral quantitative computed tomographic and densitometric analysis of skeletal tissue in male Wistar rats after chromium sulfate treatment

Bieńko¹,

Radzki²,

Wolski³

2017

Ann Agric Environ Med.

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Introduction. This study evaluates the effects of three different doses of chromium sulphate on bone density and the tomographic parameters of skeletal tissue of rats. Materials and method. The experiment was performed on 40 male Wistar rats which received, by gavage, during 90 days, a chromium sulphate in either a daily dose of 400, 600 or 800 µg/kg BW. At the end of experiment, the rats were scanned using the densitometry method (DXA) to determine the bone mineral density, bone mineral content of total skeleton and vertebral column (L2-L4) and parameters of body composition (Lean Mass and Fat Mass). The isolated femora were scanned using peripheral a quantitative computed tomography method (pQCT) for a separate analysis of the trabecular and cortical bone tissue. The ultimate strength, work to ultimate and the Young modulus of femora was also investigated by the threepoint bending test. Results. The negative impact of chromium was observed in relation to bone tissue. All doses significantly decreased total skeleton density and mineral content, and also had impact upon the isolated femora and vertebral column. Trabecular volumetric bone mineral density and trabecular bone mineral content measured by pQCT in distal femur metaphysis were significantly lower in the experimental groups than in the control. Higher doses of chromium also significantly decreased values of ultimate strength and Young modulus in the investigated femora. Conclusions. The results of the experiment demonstrate that chromium sulphate is dose dependent, and exerts a disadvantageous effect on the skeleton, as it decreases bone density and resistance.

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Section: Discussionsupporting

confidence: 82%

“…In the experiment undertaken by De Lucca et al [12] involving juvenile rats, in which hexavalent chromium was given as 6.25 and 12.5 mg/kg/10 days dosages, toxic effects were noticed on bone formation. These were manifested as decreases of mandibular growth and delays in tooth eruption.…”

Section: Discussionmentioning

confidence: 99%

The peripheral quantitative computed tomographic and densitometric analysis of skeletal tissue in male Wistar rats after chromium sulfate treatment

Bieńko¹,

Radzki²,

Wolski³

2017

Ann Agric Environ Med.

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“…Cr(VI) was also reported to alter mandibular growth and tooth eruption in rats (De Lucca et al, 2009). In this study, 4-day-old suckling Wistar pups received gavage doses of 0, 6.25 or 12.5 mg potassium dichromate/kg b.w.…”

Section: Developmental and Reproductive Toxicitymentioning

confidence: 92%

Scientific Opinion on the risks to public health related to the presence of chromium in food and drinking water

2014

EFS2

101

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EFSA received a request from the Hellenic Food Authority for a scientific opinion on estimation of the risk to human health from the presence of chromium (Cr) in food, particularly in vegetables, and Cr(VI) in bottled water. The CONTAM Panel derived a TDI of 0.3 mg/kg b.w. per day for Cr(III) from the lowest NOAEL identified in an NTP chronic oral toxicity study in rats. Under the assumption that all chromium in food is Cr(III), the mean and 95th percentile dietary exposure across all age groups were well below the TDI and therefore does not raise concerns for public health. In the case of drinking water, the Panel considered all chromium in water as Cr(VI). For non‐neoplastic effects the lowest BMDL10 for diffuse epithelial hyperplasia of duodenum in female mice and the lowest BMDL05 for haematotoxicity in male rats in a 2‐year NTP study were selected as reference points. The MOEs indicate that for non‐neoplastic effects the current exposure levels to Cr(VI) via drinking water are of no concern for public health. For neoplastic effects, the CONTAM Panel selected a lowest BMDL10 for combined adenomas and carcinomas of the mouse small intestine as the reference point. Overall, the calculated MOEs indicate low concern regarding Cr(VI) intake via drinking water (water intended for human consumption and natural mineral waters) for all age groups when considering the mean chronic exposure values with the exception of infants at the upper bound (UB) exposure estimates. MOEs below 10 000 were calculated at the UB 95th percentile exposure estimates, particularly for ‘Infants’, ‘Toddlers’ and ‘Other children’, which were highly influenced by the relatively high occurrence values under the UB assumption. To improve the risk assessment, there is a need for data on the content of Cr(III) and Cr(VI) in food and drinking water.

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“…Moreover, defects in skeletal development in children born in contaminated areas close to leather tanning processing plants have been reported 24 . Previous studies conducted by our research group have shown that tooth eruption is delayed in suckling rats exposed to hexavalent chromium 18,19 . Nevertheless, the effects of CrVI exposure on modeling and remodeling of the walls of the developing alveolus at the different stages of tooth eruption remain unknown.…”

Section: Introductionmentioning

confidence: 71%

Hexavalent chromium exposure alters bone remodeling in the developing tooth alveolus and delays tooth eruption

Sánchez¹,

Ubios²

2021

Acta Odontol Latinoam

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Although it has been demonstrated that exposure of lactating rats to CrVI delays tooth eruption, the effects of CrVI exposure on bone remodeling in the developing alveolus during tooth eruption remain unknown. Our purpose was to analyze the effect of CrVI in the alveolus of the first lower molar of rats. Thirty-two suckling Wistar rats were divided into two groups. The experimental group received 12.5 mg/kg body weight/day of potassium dichromate dissolved in saline solution by oral gavage as of day 4 of the experiment; the control group received an equal dose of saline solution. Each group was divided into two sub-sets and euthanized at the ages of 9 and 15 days, respectively. Histochemical and histomorphometric studies of the bone surfaces of the developing tooth alveolus were performed. The percentage of bone formation surfaces was lower in experimental animals than in age-matched controls. The percentage of bone resorption surfaces was significantly lower in 9-day-old experimental rats than in controls and significantly higher in 15-day-old experimental rats than in controls. Exposure to CrVI during lactation alters the sequence of bone resorption and formation in the walls of the developing alveolus, both of which are necessary for tooth eruption, thus causing a delay.

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Effect of different doses of hexavalent chromium on mandibular growth and tooth eruption in juvenile Wistar rats

Cited by 14 publications

References 29 publications

The peripheral quantitative computed tomographic and densitometric analysis of skeletal tissue in male Wistar rats after chromium sulfate treatment

The peripheral quantitative computed tomographic and densitometric analysis of skeletal tissue in male Wistar rats after chromium sulfate treatment

Scientific Opinion on the risks to public health related to the presence of chromium in food and drinking water

Hexavalent chromium exposure alters bone remodeling in the developing tooth alveolus and delays tooth eruption

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