Effect of Different Edible Trichosanthes Germplasm on Its Seed Oil to Enhance Antioxidant and Anti-Aging Activity in Caenorhabditis elegans

Wang, Wenqian; Li, Shan; Zhu, Yunguo; Zhu, Ruilin; Du, Xiling; Cui, Xianghuan; Wang, Hongbing; Cheng, Zhou

doi:10.3390/foods13030503

Foods

2024

DOI: 10.3390/foods13030503

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Effect of Different Edible Trichosanthes Germplasm on Its Seed Oil to Enhance Antioxidant and Anti-Aging Activity in Caenorhabditis elegans

Wenqian Wang,

Shan Li,

Yunguo Zhu

et al.

Abstract: The seeds of various Trichosanthes L. plants have been frequently used as snacks instead of for traditional medicinal purposes in China. However, there is still a need to identify the species based on seeds from Trichosanthes germplasm for the potential biological activities of their seed oil. In this study, 18 edible Trichosanthes germplasm from three species were identified and distinguished at a species level using a combination of seed morphological and microscopic characteristics and nrDNA-ITS sequences. … Show more

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2024

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Cited by 3 publications

References 32 publications

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Study on HPLC fingerprint of Saposhnikovia divaricata lipophilic components and anti-aging effects

Yue,

Xu,

Sun

et al. 2024

Sci Rep

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Saposhnikovia divaricata is commonly used in clinical practice as a heat-clearing and detoxifying traditional Chinese medicine. Current research on Saposhnikovia divaricata has mostly focused on chromones, while studies on its lipophilic parts remain scarce. In this study, samples of the lipophilic components of Saposhnikovia divaricata (SDL) from different regions in China were collected to establish an HPLC fingerprint. The quality of SDL was evaluated through chemical pattern recognition and multi-component content determination. Furthermore, using the Caenorhabditis elegans (C. elegans ) model, the anti-aging ability of SDL was preliminarily studied. In the HPLC fingerprints of 11 batches of SDL, 19 common peaks were identified, and 4 components were characterized as follows: peak 14, linolenic acid (ALA); peak 15, docosahexaenoic acid (DHA); peak 16, linoleic acid (LA); peak 17, oleic acid (OA). The similarity of the fingerprint ranged from 0.704 to 0.929. The results indicate that the content of ALA, DHA, LA, and OA are 0.040–0.440 mg/g, 0.003–0.116 mg/g, 1.047–6.460 mg/g, and 0.040–0.294 mg/g, respectively. HCA analysis divided 11 batches of SDL into two categories, and PCA analysis extracted 6 principal components with a cumulative contribution rate of 85.91%. The anti-aging ability of the SDL was proved by measuring the lifespan, reproductive capacity, body length, mobility, lipofuscin content, and heat stress resistance of C. elegans . The combination of high-performance liquid chromatography fingerprint and chemical pattern recognition for multi-component content determination is a reliable, comprehensive, and promising evaluation of the quality of SDL. The confirmation of SDL’s anti-aging ability provides the possibility for the research of Saposhnikovia divaricata in the field of healthy food and cosmetics.

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Study on HPLC fingerprint of Saposhnikovia divaricata lipophilic components and anti-aging effects

Yue,

Xu,

Sun

et al. 2024

Sci Rep

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Antioxidant and Neuroprotective Effects of Seed Oils from Trichosanthes kirilowii and T. laceribractea in Caenorhabditis elegans: A Comparative Analysis and Mechanism Study

Wang,

Li,

Zhu

et al. 2024

Antioxidants

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Excess reactive oxygen species (ROS) can accelerate amyloid β (Aβ) aggregation and tau protein hyperphosphorylation in neuron cells, which further leads to neurodegenerative diseases such as Alzheimer’s disease (AD). Therefore, there is an urgent need to find natural and safe antioxidants for preventing or treating such neurodegenerative diseases. The seeds of Trichosanthes kirilowii Maxim and T. laceribractea Hayata have long been used for medicinal and edible purposes in China. However, the antioxidant and neuroprotective activities and underlying mechanisms of their seed oils still remain unclear. Herein, we examine the antioxidant and neuroprotective effects of seed oils extracted from different germplasms, T. kirilowii (YNHH and SDJN) and T. laceribractea (ZJQT and SXHZ), on ROS levels and neuroprotective activities in C. elegans. The results demonstrated that the seed oils significantly reduced the ROS levels in C. elegans by 17.03–42.74%, with T. kirilowii (YNHH and SDJN) exhibiting significantly stronger ROS scavenging abilities than T. laceribractea (ZJQT and SXHZ). The seed oils from T. kirilowii (YNHH and SDJN) alleviated the production and aggregation of Aβ and the phosphorylation and polymerization of tau, suggesting a potential neuroprotective role. Conversely, seed oils from T. laceribractea (ZJQT and SXHZ) show minimal neuroprotective effects in C. elegans. These differential outcomes might stem from distinct mechanisms underlying antioxidant and neuroprotective effects, with the ctl-2 gene implicated as pivotal in mediating the significant neuroprotective effects of seed oils from T. kirilowii (YNHH and SDJN). Our findings have provided valuable insights into the antioxidant and neuroprotective properties of T. kirilowii seed oils, paving the way for further research aimed at elucidating the underlying mechanisms and exploring their potential therapeutic applications in combating neurodegenerative diseases.

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Optimization of the Process of Extracting Polysaccharides from Agrocybe aegerita and In Vitro Antioxidant and Anti-Aging Tests

Wang,

Yang,

et al. 2024

Molecules

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The extraction process of crude polysaccharides from Agrocybe aegerita was optimized, and the antioxidant and anti-aging effects of the crude polysaccharides were evaluated. The optimal extraction parameters for the polysaccharide were identified using the response surface methodology. The DPPH, hydroxy radical, and superoxide anion radical scavenging capacities were evaluated to determine the antioxidant properties of the AAPs. The effects of the AAPs on the lifespan, head-swing frequency, swallowing frequency, body-bending frequency, and stress resistance of Caenorhabditis elegans were determined. The optimal extraction conditions included a solid-to-liquid ratio that resulted in an extraction solution concentration of 0.034 g/mL, an extraction temperature of 92.64 °C, and an extraction time of 2.82 h. Under these conditions, the yield of the AAPs was 11.325% ± 0.996%. The IC50 of the AAPs for superoxide anion radical scavenging was 4.693 mg/mL. The AAPs reached their maximum activity at concentrations of about 2 mg/mL for DPPH and 5 mg/mL for the hydroxyl radical. The AAPs could prolong the lifespan and improve locomotion and the stress resistance of C. elegans. Our findings illustrate the potential of AAPs as an anti-aging and antioxidant agent, highlighting the use of this natural compound in the fields of food and pharmacology.

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Effect of Different Edible Trichosanthes Germplasm on Its Seed Oil to Enhance Antioxidant and Anti-Aging Activity in Caenorhabditis elegans

Cited by 3 publications

References 32 publications

Study on HPLC fingerprint of Saposhnikovia divaricata lipophilic components and anti-aging effects

Study on HPLC fingerprint of Saposhnikovia divaricata lipophilic components and anti-aging effects

Antioxidant and Neuroprotective Effects of Seed Oils from Trichosanthes kirilowii and T. laceribractea in Caenorhabditis elegans: A Comparative Analysis and Mechanism Study

Optimization of the Process of Extracting Polysaccharides from Agrocybe aegerita and In Vitro Antioxidant and Anti-Aging Tests

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