Effect of different estrus synchronization protocols on estrus and pregnancy rates, oxidative stress and some biochemical parameters in Hair goats

Eşki, Funda; Kurt, Serdal; Demir, Pınar

doi:10.1016/j.smallrumres.2021.106348

Cited by 7 publications

(5 citation statements)

References 36 publications

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“…The estrous cycle of female animals is affected by external and internal physiological factors [34,35]. To explore the gene expression differences at different estrus stages in female rabbits, we analyzed the ovarian transcription patterns of female rabbits between diestrus and estrus.…”

Section: Discussionmentioning

confidence: 99%

Behavioral and Physiological Differences in Female Rabbits at Different Stages of the Estrous Cycle

Chen,

Jin,

Yang

et al. 2023

Animals

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Estrus involves a series of complex physiological signs and changes in behavior before ovulation, which play a crucial role in animal reproduction. However, there have been few studies that evaluate behaviors during the different stages of estrus cycle in female rabbits. Therefore, more detailed information is needed on distinguishing the various stages of the estrous cycle. This study explored the behavioral and physiological differences at various estrous cycle stages in female New Zealand White rabbits. The continuous recording method was employed to record the daily behaviors of twenty postpartum female rabbits during the estrous cycle. Compared with the diestrus stage, the duration of foraging and drinking behavior in estrus decreased significantly, and the frequency of grooming and biting behaviors increased (p < 0.05). Differences in reproductive hormone levels (FSH, LH, P4, and E2) and follicle development were measured at each stage via ELISA and HE staining. The FSH and LH levels showed an increasing trend and then decreased, with the lowest being in late estrus (p < 0.05). The P4 level was the lowest in estrus (p < 0.05), and E2 showed a gradually increasing trend. There was no significant difference in the number of primordial follicles at each stage, but the number of primary follicles in estrus was significantly higher than at the other stages (p < 0.05). To further understand the molecular regulation mechanism of the estrous cycle in female rabbits, we analyzed the ovarian transcription patterns of female rabbits in diestrus (D group) and estrus (E group) employing RNA-seq. A total of 967 differentially expressed genes (DEGs) were screened from the ovaries of female rabbits between the diestrus and estrus groups. A KEGG analysis of DEGs enriched in the estrogen signaling pathway, aldosterone synthesis, and secretion pathway, such as CYP19A1 and IGF1R, was performed. The rabbits’ behavior, related physiological hormones, and molecular regulation also differed at different estrous cycle stages. The results provide recommendations for the adequate management practices of postpartum re-estrus and breeding female rabbits.

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Section: Discussionmentioning

confidence: 99%

Behavioral and Physiological Differences in Female Rabbits at Different Stages of the Estrous Cycle

Chen,

Jin,

Yang

et al. 2023

Animals

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“…However, a difference in ovarian activity was expected in the pregnant group due to the hormonal mechanism of pregnancy. Purpose of including this group in the study was to reveal the effect of high P4 and pregnancy on kisspeptin and oxidative stress levels and the difference of the group with CEH from the pregnant group as well as healthy group, because it is known that some hormones and pregnancy can affect kisspeptin level (Clarkson and Herbison, 2009; Mondal et al., 2015; Leite et al, 2016; Cao et al., 2019), and oxidative stress (Vannucchi et al., 2007; Eşki et al, 2021). On the other hand, it is known that steroid hormones such as estrogen and progesterone play an important role on the CEH (Bigliardi et al., 2004; De Bosschere et al., 2002).…”

Section: Discussionmentioning

confidence: 99%

“…The levels of these biochemical parameters differ in many pathological conditions, so it can be considered as an indicator of general health. Moreover, TSH level is associated with both reproductive health and oxidative stress (Eşki et al, 2021). In this study, when some important health parameters including TSH, ALT, AST, ALP, LDH, CRE and BUN were examined to clarify the difference of kisspeptin in dogs with CEH and healthy, it was found that all of these parameters are similar between CEH and healthy groups.…”

Section: Discussionmentioning

confidence: 99%

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Investigation of the usability of kisspeptin and oxidative stress parameters in the early diagnosis of asymptomatic cystic endometrial hyperplasia in dogs

Kurt

Eşki

Mıs

2021

Reprod Domestic Animals

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This study aimed to investigate the differences in oxidative stress index (OSI) and kisspeptin levels in clinically asymptomatic dogs with cystic endometrial hyperplasia (CEH) compared to healthy and pregnant dogs, and to determine the usability of the obtained results in the diagnosis of asymptomatic CEH. The study comprised three groups; a healthy (n = 8), a pregnant (n = 10) and a CEH (n = 10). All dogs in the three groups were included in the study at the 30 ± 3th day after estrus, and blood samples were collected for analysis of kisspeptin, total antioxidant status (TAS), total oxidant status (TOS), progesterone (P4), estradiol (E2) and some biochemical parameters (TSH; thyroid stimulating hormone, ALT; alanine aminotransferase, AST; aspartate aminotransferase, ALP; alkaline phosphatase, LDH; lactate dehydrogenase, CRE; creatine and BUN; blood urea nitrogen). In addition, OSI value was calculated. P4 and ALT and BUN levels were significantly lower and higher in CEH group than the pregnant group, respectively (p < .05). While kisspeptin and TAS levels were significantly lower in CEH group compared to the healthy and pregnant groups (p < .01), OSI level increased dramatically. In conclusion, it was confirmed that CEH clearly affected kisspeptin and OSI levels, and it is thought that these parameters may be an alternative diagnostic tool for the detection of CEH after further studies.

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“…Firstly, unit of TAS (mmol Trolox eq/L) was converted to µmol Trolox eq/L. Thus, the TAS and TOS units were made identical (µmol Trolox eq/L) and the OSI value was calculated with the following formula: TOS / TAS x 100 [46].…”

Section: Calculation Of Oxidative Stress Index (Osi)mentioning

confidence: 99%

Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats

Kurt¹,

SEKER²,

YAZLIK³

et al. 2023

jrp

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The study investigated the major phenolic compounds of Aloe vera leaf (AVL) by the high-performance liquid chromatography (HPLC) method, and its protective effect on ovaries under oxidative stress of streptozotocininduced diabetic rats. The study was conducted on control (untreated healthy rats; C), diabetes (untreated diabetic rats; D) and diabetes+A. vera treatment (diabetic rats treated with A. vera; D+A) groups. D+A group was given an ethanolic extract of A. vera (300 mg/kg) for 14 days. Major phenolic compounds of AVL were chlorogenic acid and rutin. alanine transaminase (ALT) and aspartate aminotransferase (AST) levels increased in D group compared to others groups (P<0.01). Alkaline phosphatase (ALP) and albumin levels were lower and higher in the C group than in other groups, respectively (P<0.01). In the D group compared to other groups, oxidative stress index (OSI) and total oxidant status levels increased, while total antioxidant status level decreased in both ovarian tissues and blood (P<0.01). Preantral and antral follicle counts decreased in the D group according to other groups (P<0.01) and atretic follicle count increased (P<0.05). Proliferating cell nuclear antigen (PCNA) expression level (P<0.01), and B-cell lymphoma-2-associated-Xprotein (Bax; P<0.01) and tumor necrosis factor-alpha (TNF-α) expression levels (P<0.05) decreased and increased in group D according to other groups, respectively. In conclusion, treatment with AVL decreased OSI, improved follicular dynamics, and restored Bax, TNF-α and PCNA expression in diabetic rats.

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Effect of different estrus synchronization protocols on estrus and pregnancy rates, oxidative stress and some biochemical parameters in Hair goats

Cited by 7 publications

References 36 publications

Behavioral and Physiological Differences in Female Rabbits at Different Stages of the Estrous Cycle

Behavioral and Physiological Differences in Female Rabbits at Different Stages of the Estrous Cycle

Investigation of the usability of kisspeptin and oxidative stress parameters in the early diagnosis of asymptomatic cystic endometrial hyperplasia in dogs

Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats

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