Effect of different factors on the recovery rate of oocytes for in vitro maturation and in vitro fertilisation procedures in sheep

Wani, Nisar Ahmad; Wani, G. M.; Khan, M.Z.; Sidiqi, M.A

doi:10.1016/s0921-4488(99)00046-2

Cited by 29 publications

(25 citation statements)

References 8 publications

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“…Based on the present results the recovery of oocytes using the slicing and puncture methods yielded more oocytes per ovary and more good-quality oocytes per ovary than the two aspiration methods (P < 0.05). In accordance with the results of the present study on Boer goat, Wani et al (1999) indicated that slicing (9.5 ± 0.4) and puncture (9.5 ± 0.4) yielded significantly (P < 0.05) more oocytes per ovary than follicle aspiration (6.8 ± 0.3) in sheep. The higher number of good quality oocytes which had been recovered per ovary by slicing (5.2 ± 0.2) and puncture (5.2 ± 0.2) compared to aspiration (4.4 ± 0.2) was also in accordance with the results reported here.…”

Section: Discussionsupporting

confidence: 92%

“…Several techniques were used for the collection of oocytes from the ovaries in goats (Mogas et al, 1992;Pawshe et al, 1994) and sheep (Wani et al, 1999). Based on the present results the recovery of oocytes using the slicing and puncture methods yielded more oocytes per ovary and more good-quality oocytes per ovary than the two aspiration methods (P < 0.05).…”

Section: Discussionsupporting

confidence: 50%

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Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat

Wang¹,

Xu²,

Yu³

2007

CZECH J ANIM SCI

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ABSTRACT:The oocytes (experiment 1) were harvested by one of the four collection techniques (slicing, puncture, aspiration I and aspiration II) and the total number and the number of each grade of oocytes were counted, respectively. The good-quality oocytes (good and fair grade) were cultured for maturation. In experiment 2, the oocytes were matured in TCM-199 supplemented with 10 ng/ml of epidermal growth factor (EGF) or 10% FCS, either alone or with 1 IU/ml FSH, or the oocytes were matured in TCM-199 supplemented with 1 IU/ml FSH. After maturation, the oocytes in the two experiments were fertilized, respectively. Slicing (6.3) and puncture (5.8) of the ovaries yielded a higher (P < 0.05) number of oocytes per ovary compared to aspiration I (2.9) and aspiration II (3.1). Oocytes matured in the TCM-199 medium supplemented with EGF or FCS with FSH had a significantly higher proportion of blastocysts than the other treatments (P < 0.05). In conclusion, slicing and puncture are alternative techniques of oocyte recovery in Boer goat. The TCM-199 medium supplemented with EGF or FCS in the presence of FSH is suitable for in vitro maturation of oocytes.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 50%

Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat

Wang¹,

Xu²,

Yu³

2007

CZECH J ANIM SCI

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“…Oocytes were collected using a combination of puncture and slicing techniques (Wani et al, 1999) in a Petri dish that contained handling medium (Hepesbuffered TCM-199 supplemented with 0.1% of polyvinyl alcohol, 0.04% of sodium bicarbonate, 25 IU mL -1 of heparin, 100 IU mL -1 of penicillin-G, and 100 µg mL -1 of streptomycin sulphate). Following Wani et al (2000), oocytes were categorized based on their cumulus cells and cytoplasm morphology; only the oocytes that had several layers of cumulus cells and a uniform cytoplasm were selected for in vitro maturation.…”

Section: Oocyte Collection and In Vitro Maturationmentioning

confidence: 99%

The effects of melatonin on in vitro oocyte competence and embryo development in sheep

Casao

Abecia

Pérez

et al. 2010

Span. j. agric. res.

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The aim of this study was to assess the effects of melatonin on the in vitro maturation and fertilization of sheep oocytes, and on the in vitro culture of the embryos. Oocytes from sheep ovaries collected at the slaughterhouse were divided into four groups, two of which were treated with either 10E5 M (M5) or 10E6 M (M6) melatonin, while the other two groups served as untreated controls (C5 and C6). After in vitro fertilization with fresh ram semen, the embryos produced in each group were divided into two sets, one cultured with melatonin (M5M, C5M, M6M and C6M), and the other without melatonin (M5C, C5C, M6C, and C6C). A melatonin concentration of 10E6 M increased maturation rate (82.5% vs. 73.7% for M6 and C6, respectively; P < 0.05) and tended to improve cleavage rate 36 hours after in vitro fertilization (79.4% vs. 72.6% for M6 and C6, respectively, P = 0.08). A higher melatonin concentration (10E5 M) did not have significant effects on those parameters. Blastocyst rates on day 8 did not differ significantly among groups.

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“…The comparison between these two techniques has been performed on bovine [4] [5], equine [6] [7], goats [8], sheep [9] and camels [10], but not in swine.…”

Section: Introductionmentioning

confidence: 99%

Effect of Oocyte Recovery Techniques on <i>in Vitro</i> Production of Swine Embryos

Marques¹,

Barros²,

Goissis³

et al. 2015

OJAS

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In vitro production of swine embryos is a valuable tool to generate clones and genetically modified pigs during a short period of time. However, the efficiency of the existing methods is extremely low and the oocyte quality and quantity represent important obstacles on the success of in vitro production of embryos. Therefore, the aim of this study was to compare the in vitro maturation, fertilization and subsequent embryo development rates of oocytes recovered by ovary slicing or follicular aspiration. The oocyte recovery rate (grade 1 COC/ovary) was higher (p = 0.0083) in the slicing group when compared to the aspiration group. No differences were observed between groups regarding in vitro maturation and early cleavage rates. A higher percentage of oocytes recovered by follicular aspiration reached the blastocyst stage after IVF when compared to the ovary slicing method (p = 0.0395). However, no difference on blastocyst cell number was observed. Although the recovery of oocytes using the slicing technique yielded more grade 1 oocytes per ovary than the aspiration method, the number of oocytes that reached the blastocyst stage after IVF by the slicing method was lower when compared with oocytes obtained by aspiration, as observed by lower blastocyst rates. In conclusion, the follicular aspiration is the method of choice for porcine in vitro embryo production.

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Effect of different factors on the recovery rate of oocytes for in vitro maturation and in vitro fertilisation procedures in sheep

Cited by 29 publications

References 8 publications

Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat

Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat

The effects of melatonin on in vitro oocyte competence and embryo development in sheep

Effect of Oocyte Recovery Techniques on <i>in Vitro</i> Production of Swine Embryos

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Effect of different factors on the recovery rate of oocytes for in vitro maturation and in vitro fertilisation procedures in sheep

Cited by 29 publications

References 8 publications

Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat

Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat

The effects of melatonin on in vitro oocyte competence and embryo development in sheep

Effect of Oocyte Recovery Techniques on &lt;i&gt;in Vitro&lt;/i&gt; Production of Swine Embryos

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Effect of Oocyte Recovery Techniques on <i>in Vitro</i> Production of Swine Embryos