EFFECT OF DIFFERENT FACTORS ON IN VITRO MULTIPLICATION OF LEUCADENDRON ÂSAFARI SUNSETâ (PROTEACEAE)

Pérez-Francés, J.F.; Expósito, Alejandro; Rodríguez, José Sánchez

doi:10.17660/actahortic.1995.387.13

Cited by 7 publications

(8 citation statements)

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“…No differences in tissue oxidation were observed between the different treatments. Results during the multiplication phase were similar to those reported by Pérez-Francés et al (1995);nevertheless Dias Ferreira et al (2003) obtained higher multiplication rates, although they used a combination of different growth regulators. Buds obtained in a medium with 2.2 µM BAP browned rapidly due to their poor development.…”

Section: Discussionsupporting

confidence: 87%

“…A previous report by Pérez-Francés et al (1995) showed the effect of some factors on in vitro multiplication of this cultivar. In this study, the use of benzylaminopurine (BAP) improved bud proliferation rates.…”

Section: Introductionmentioning

confidence: 74%

“…Darkening can be reduced by limiting the production of phenolic compounds, modifying the redox potential, inactivating the phenolases, and reducing the activity of the phenolase and/or the availability of substrate (Hu and Wang, 1983). In several reports concerning Proteaceae, the use of an antioxidant solution is necessary in some phases of culture: citric and/or ascorbic acid (Pérez-Francés et al, 1995;Dias Ferreira et al, 2003;Wu and du Toit, 2004), PVP (Pérez-Francés et al, 2001a,b), or BAP applied to the entire mother plant at 200 mg L -1 . This latter significantly reduced browning and promoted bud sprouting in vitro (Rugge, 1995;Pérez-Francés et al, 2001b).…”

Section: Discussionmentioning

confidence: 99%

“…The 1/2 MS medium was supplemented with plant growth regulators and the antioxidant substances and adjusted to pH 5.7 before autoclaving. Antioxidant treatments were used in the establishment phase to reduce explant oxidation: a combination of citric (1,500 mg L -1 ) and ascorbic acid (100 mg L -1 ) (Pérez-Francés et al, 1995) or polyvinylpyrrolidone (PVP) (1 g L -1 ), employed in different ways: T1 (ascorbic and citric acid solution shaken for 30 min before culture), T2 (T1 followed by darkness for three days after culture), T3 (T1 then subculture every three days during the first week of culture), T4 (ascorbic and citric acid included in the medium), P1 (PVP solution shaken as in T1), P2 (P1, then treated as in T2), P3 (P1 then subcultured as in T3), P4 (as T4 but with PVP), O (as T2 without antioxidants), SF (subcultured as in T3 without antioxidants). The cultures were maintained for 8 weeks during which they were transferred once after 4 weeks of incubation.…”

Section: Establishmentmentioning

confidence: 99%

“…Although diverse authors have studied the micropropagation of some proteas (Hu and Wang, 1983;BenJaacov and Jacobs, 1986;Seelye et al, 1986;Rugge et al, 1989;Kunisaki, 1989Kunisaki, , 1990Bunn and Dixon, 1992a,b;Watad et al, 1992a,b;Pérez-Francés et al, 1995;Rugge, 1995;Dias Ferreira et al, 2003;Wu and du Toit, 2004), results have not been totally satisfactory, as at present there is still no firm that specialises in marketing in vitro produced protea plants. A previous report by Pérez-Francés et al (1995) showed the effect of some factors on in vitro multiplication of this cultivar.…”

Section: Introductionmentioning

confidence: 99%

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Use of multinodal explants for micropropagation of Leucadendron ‘Safari Sunset’

Suárez

Pérez-Francés

Rodríguez-Pérez

2010

Span. j. agric. res.

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Leucadendron 'Safari Sunset' is one of the most valuable Proteaceae cultivar, due to its economic importance as cutflower. In vitro tissue culture of this cultivar and others may offer a massive cloned plant production that permit to satisfy the market demand. Multinodal explants of Leucadendron 'Safari Sunset' were established in vitro on a half strength Murashige and Skoog medium supplemented with 2.2 µM benzylaminopurine (BAP). Various antioxidant treatments were used to reduce the presence of phenolic compounds in culture. No important differences in growth and multiplication between these treatments were detected during in vitro establishment and multiplication, but explants cultured on a medium with 1 g L -1 of polyvinylpyrrolidone (PVP) developed a higher number of buds in the establishment phase. Proliferation of axillary shoots was achieved with 0.88 µM BAP, being slightly higher on a medium with 2.2 µM BAP although the buds were deformed, showing abnormal leaves. The use of phytagel in this phase increased proliferation rate and shoot growth. Low percentages rooting were obtained when microcuttings were cultured on the same basal medium supplemented with several indole-3-butyric acid (IBA) concentrations. The best rooting percentage (37.5%) was obtained with 44.1 µM IBA. This work shows that micropropagation of L. 'Safari Sunset' is possible with multinodal explants, although is necessary to optimize the protocol for commercial applications.Additional key words: antioxidant treatment, in vitro tissue culture, ornamental plants, phenolic compounds, Proteaceae. ResumenEmpleo de explantos multinodales para la micropropagación de Leucadendron 'Safari Sunset' Leucadendron 'Safari Sunset' es uno de los cultivares más importantes de Proteaceae debido a su importancia económica como flor cortada. El cultivo in vitro de este cultivar y otros, puede ofrecer una producción masiva de clones que permita satisfacer la demanda del mercado. Se establecieron in vitro explantos multinodales de L. 'Safari Sunset' en un medio Murashige y Skoog, con los macronutrientes a la mitad de su concentración, y bencilaminopurina (BAP) 2,2 µM. Para reducir la presencia de compuestos fenólicos en los cultivos, se emplearon diferentes tratamientos antioxidantes. No se observaron diferencias importantes en cuanto al crecimiento y multiplicación de las yemas, entre los antioxidantes empleados durante el establecimiento y multiplicación in vitro, sin embargo, se obtuvo un mayor nú-mero de yemas (25 ± 2,82) en los explantos sembrados en un medio con 1 g L -1 de polivinilpirrolidona (PVP). La multiplicación de las yemas se logró con BAP 0,88 µM. La tasa de proliferación fue ligeramente superior al adicionar al medio de cultivo BAP 2,2 µM, pero en este medio se desarrollaron yemas deformes, con hojas anormales. El empleo de fitagel incrementó la tasa de multiplicación y crecimiento de los vástagos. Se obtuvieron bajos porcentajes de enraizamiento al cultivar los microesquejes en el medio basal suplementado con diferentes concentraci...

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Establishmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Use of multinodal explants for micropropagation of Leucadendron ‘Safari Sunset’

Suárez

Pérez-Francés

Rodríguez-Pérez

2010

Span. j. agric. res.

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Banksia:New Proteaceous Cut Flower Crop

Sedgley¹

1998

Horticultural Reviews

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More than 1400 species have been recognized in the ancient Proteaceae family (Rebelo 1995). Their occurrence is mostly distributed between Australia with about 800 species and Africa with about 400 species with the remainder found in South America, the islands east of New Guinea, and a few species in southeast Asia, New Zealand, and Madagascar. They are broadly referred to as proteas, although we identify specific genera by their Latin names. The subfamily Proteoideae, largely found in Africa, has contributed the genera Protea, Leucadendron, and Leucospermum to floricultural trade, while the Australian Grevilleoideae has contributed Banksia and Grevillea that have found similar use in floriculture and landscaping. Other genera are still emerging in importance (Criley, 2001

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